Bicelles, an artificial disk-shaped lipid bilayer, are commonly used for the structural and functional characterization of membrane-bound proteins in an environment similar to that in intracellular membranes. Because the dynamics of the lipids that constitute bicelles exert a significant impact on the structure and function of the inserted proteins, we investigated the mobility of lipid molecules in bicelles composed of DMPC (14:0 PC) and DHPC (06:0 PC) using solution NMR and MD calculations....
[NMR paper] Characterization of fast-tumbling isotropic bicelles by PFG diffusion NMR.
Characterization of fast-tumbling isotropic bicelles by PFG diffusion NMR.
Characterization of fast-tumbling isotropic bicelles by PFG diffusion NMR.
Magn Reson Chem. 2015 Dec 14;
Authors: Liebau J, Ye W, Mäler L
Abstract
Small isotropic bicelles are versatile membrane mimetics, which, in contrast to micelles, provide a lipid bilayer and are at the same time suitable for solution-state NMR studies. The lipid composition of the bilayer is flexible allowing for incorporation of various head groups and acyl chain types. In...
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Isotropic Bicelles Stabilize the Juxtamembrane Regionof the Influenza M2 Protein for Solution NMR Studies
Isotropic Bicelles Stabilize the Juxtamembrane Regionof the Influenza M2 Protein for Solution NMR Studies
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/bi401035m/aop/images/medium/bi-2013-01035m_0009.gif
Biochemistry
DOI: 10.1021/bi401035m
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11-15-2013 12:34 AM
[NMR paper] Solution NMR analysis of the interaction between the actinoporin Sticholysin I and DHPC micelles. Correlation with backbone dynamics.
Solution NMR analysis of the interaction between the actinoporin Sticholysin I and DHPC micelles. Correlation with backbone dynamics.
Related Articles Solution NMR analysis of the interaction between the actinoporin Sticholysin I and DHPC micelles. Correlation with backbone dynamics.
Proteins. 2013 Nov 12;
Authors: Castilla AL, Santos FP, Schreier S, Pires JR
Abstract
Sticholysin I (StI), an actinoporin expressed as a water-soluble protein by the sea anemone Stichodactyla helianthus, binds to natural and model membranes, forming oligomeric...
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11-13-2013 09:22 PM
[NMR paper] Isotropic bicelles stabilize the juxtamembrane region of the influenza M2 protein for solution NMR studies.
Isotropic bicelles stabilize the juxtamembrane region of the influenza M2 protein for solution NMR studies.
Related Articles Isotropic bicelles stabilize the juxtamembrane region of the influenza M2 protein for solution NMR studies.
Biochemistry. 2013 Oct 29;
Authors: Claridge JK, Aittoniemi J, Cooper DM, Schnell JR
Abstract
The protein M2 from influenza is a tetrameric membrane protein with several roles in the viral life cycle. The transmembrane helix (TMH) of M2 has proton channel activity that is required for unpackaging the viral...
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10-31-2013 02:18 PM
[NMR paper] The Three Dimensional Structure and Interaction Studies of HCV p7 in DHPC by Solution NMR.
The Three Dimensional Structure and Interaction Studies of HCV p7 in DHPC by Solution NMR.
The Three Dimensional Structure and Interaction Studies of HCV p7 in DHPC by Solution NMR.
Biochemistry. 2013 Jul 10;
Authors: Cook GA, Dawson LA, Tian Y, Opella SJ
Abstract
Hepatitis C Virus (HCV) protein p7 plays an important role in the assembly and release of mature virus particles. This small 63-residue membrane protein has been shown to induce channel activity, which may contribute to its functions. p7 is highly conserved throughout the...
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07-12-2013 06:01 PM
[NMR paper] Direct observation and characterization of DMPC/DHPC aggregates under conditions rele
Direct observation and characterization of DMPC/DHPC aggregates under conditions relevant for biological solution NMR.
Related Articles Direct observation and characterization of DMPC/DHPC aggregates under conditions relevant for biological solution NMR.
Biochim Biophys Acta. 2004 Aug 30;1664(2):241-56
Authors: van Dam L, Karlsson G, Edwards K
We have used cryo-transmission electron microscopy (cryo-TEM) for inspection of aggregates formed by dimyristoylphosphatidylcholine (DMPC) and dihexanoylphosphatidylcholine (DHPC) in aqueous solution at...
Dynamic properties of isotropic DMPC/DHPC bicelles: Insights from solution NMR and MD simulations
Linear Mode
