Related ArticlesDynamic Nuclear Polarization / solid-state NMR of membranes. Thermal effects and sample geometry.
Solid State Nucl Magn Reson. 2019 Mar 22;100:70-76
Authors: Salnikov ES, Aussenac F, Abel S, Purea A, Tordo P, Ouari O, Bechinger B
Abstract
Whereas specially designed dinitroxide biradicals, reconstitution protocols, oriented sample geometries and NMR probes have helped to much increase the DNP enhancement factors of membrane samples they still lag considerably behind those obtained from glasses made of protein solutions. Here we show that not only the MAS rotor material but also the distribution of the membrane samples within the NMR rotor have a pronounced effect on the DNP enhancement. These observations are rationalized with the cooling efficiency and the internal properties of the sample, monitored by their T1 relaxation, microwave ON versus OFF signal intensities and DNP effect. The data are suggestive that for membranes the speed of cooling has a pronounced effect on the membrane properties and concomitantly the distribution of biradicals within the sample.
PMID: 30995597 [PubMed - as supplied by publisher]
Characterizing Thermal Mixing Dynamic Nuclear Polarization via Cross-Talk between Spin Reservoirs #DNPNMR
From The DNP-NMR Blog:
Characterizing Thermal Mixing Dynamic Nuclear Polarization via Cross-Talk between Spin Reservoirs #DNPNMR
Guarin, D., et al., Characterizing Thermal Mixing Dynamic Nuclear Polarization via Cross-Talk between Spin Reservoirs. The Journal of Physical Chemistry Letters, 2017. 8(22): p. 5531-5536.
https://www.ncbi.nlm.nih.gov/pubmed/29076730
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12-29-2017 09:03 PM
[NMR paper] Strategies for Efficient Sample Preparation for Dynamic Nuclear Polarization Solid-State NMR of Biological Macromolecules.
Strategies for Efficient Sample Preparation for Dynamic Nuclear Polarization Solid-State NMR of Biological Macromolecules.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Strategies for Efficient Sample Preparation for Dynamic Nuclear Polarization Solid-State NMR of Biological Macromolecules.
Methods Mol Biol. 2018;1688:133-154
Authors: Itin B, Sergeyev IV
Abstract
Solid-state NMR (SSNMR) is a powerful tool for the elucidation...
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11-21-2017 10:10 PM
Thermal annihilation of photo-induced radicals following dynamic nuclear polarization to produce transportable frozen hyperpolarized 13C-substrates
From The DNP-NMR Blog:
Thermal annihilation of photo-induced radicals following dynamic nuclear polarization to produce transportable frozen hyperpolarized 13C-substrates
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Capozzi, A., et al., Thermal annihilation of photo-induced radicals following dynamic nuclear polarization to produce transportable frozen hyperpolarized 13C-substrates. 2017. 8: p. 15757.
http://dx.doi.org/10.1038/ncomms15757
Is solid-state NMR enhanced by dynamic nuclear polarization?
From The DNP-NMR Blog:
Is solid-state NMR enhanced by dynamic nuclear polarization?
Lee, D., S. Hediger, and G. De Paepe, Is solid-state NMR enhanced by dynamic nuclear polarization? Solid State Nucl Magn Reson, 2015. 66-67C(0): p. 6-20.
http://www.ncbi.nlm.nih.gov/pubmed/25779337
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05-02-2015 02:38 AM
The role of the glassy dynamics and thermal mixing in the dynamic nuclear polarization and relaxation mechanisms of pyruvic acid
From The DNP-NMR Blog:
The role of the glassy dynamics and thermal mixing in the dynamic nuclear polarization and relaxation mechanisms of pyruvic acid
Filibian, M., et al., The role of the glassy dynamics and thermal mixing in the dynamic nuclear polarization and relaxation mechanisms of pyruvic acid. Phys Chem Chem Phys, 2014. 16(48): p. 27025-36.
http://www.ncbi.nlm.nih.gov/pubmed/25382595
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03-27-2015 11:59 PM
Effects of the electron polarization on dynamic nuclear polarization in solids
From The DNP-NMR Blog:
Effects of the electron polarization on dynamic nuclear polarization in solids
Hovav, Y., et al., Effects of the electron polarization on dynamic nuclear polarization in solids. Phys Chem Chem Phys, 2015. 17(8): p. 6053-65.
http://www.ncbi.nlm.nih.gov/pubmed/25640165
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03-23-2015 08:22 PM
Optimizing sample preparation methods for dynamic nuclear polarization solid-state NMR of synthetic polymers
From The DNP-NMR Blog:
Optimizing sample preparation methods for dynamic nuclear polarization solid-state NMR of synthetic polymers
Le, D., et al., Optimizing sample preparation methods for dynamic nuclear polarization solid-state NMR of synthetic polymers. Macromolecules, 2014: p. 140613123939001.
http://pubs.acs.org/doi/abs/10.1021/ma500788n
Dynamic Nuclear Polarization / solid-state NMR of membranes. Thermal effects and sample geometry.
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