We report the direct dynamic nuclear polarization (DNP) of 13C nuclei of a uniformly [13C,15N]-labeled, paramagnetic full-length hammerhead ribozyme (HHRz) complex with Mn2+ where the enhanced polarization is fully provided by the endogenously bound metal ion and no exogenous polarizing agent is added. A 13C enhancement factor of ĪµĀ*=Ā*8 was observed by intra-complex DNP at 9.4Ā*T. In contrast, ā??conventionalā?? indirect and direct DNP experiments were performed using AMUPol as polarizing agent where we obtained a 1H enhancement factor of ĪµĀ*ā??Ā*250. Comparison with the diamagnetic (Mg2+) HHRz complex shows that the presence of Mn2+ only marginally influences the (DNP-enhanced) NMR properties of the RNA. Furthermore two-dimensional correlation spectra (15Nā??13C and 13Cā??13C) reveal structural inhomogeneity in the frozen, amorphous state indicating the coexistence of several conformational states. These demonstrations of intra-complex DNP using an endogenous metal ion as well as DNP-enhanced MAS NMR of RNA in general yield important information for the development of new methods in structural biology.
Dynamic nuclear polarization of membrane proteins: covalently bound spin-labels at protein-protein interfaces
From The DNP-NMR Blog:
Dynamic nuclear polarization of membrane proteins: covalently bound spin-labels at protein-protein interfaces
Wylie, B.J., et al., Dynamic nuclear polarization of membrane proteins: covalently bound spin-labels at protein-protein interfaces. J Biomol NMR, 2015. 61(3-4): p. 361-7.
http://www.ncbi.nlm.nih.gov/pubmed/25828256
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06-17-2015 09:27 PM
Dynamic nuclear polarization of membrane proteins: covalently bound spin-labels at proteinā??protein interfaces
Dynamic nuclear polarization of membrane proteins: covalently bound spin-labels at proteinā??protein interfaces
Abstract
We demonstrate that dynamic nuclear polarization of membrane proteins in lipid bilayers may be achieved using a novel polarizing agent: pairs of spin labels covalently bound to a protein of interest interacting at an intermolecular interaction surface. For gramicidin A, nitroxide tags attached to the N-terminal intermolecular interface region become proximal only when bimolecular channels forms in the membrane. We obtained signal...
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04-01-2015 08:27 AM
The role of the glassy dynamics and thermal mixing in the dynamic nuclear polarization and relaxation mechanisms of pyruvic acid
From The DNP-NMR Blog:
The role of the glassy dynamics and thermal mixing in the dynamic nuclear polarization and relaxation mechanisms of pyruvic acid
Filibian, M., et al., The role of the glassy dynamics and thermal mixing in the dynamic nuclear polarization and relaxation mechanisms of pyruvic acid. Phys Chem Chem Phys, 2014. 16(48): p. 27025-36.
http://www.ncbi.nlm.nih.gov/pubmed/25382595
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03-27-2015 11:59 PM
Hyperpolarization of deuterated metabolites via remote cross-polarization and dissolution dynamic nuclear polarization
From The DNP-NMR Blog:
Hyperpolarization of deuterated metabolites via remote cross-polarization and dissolution dynamic nuclear polarization
Vuichoud, B., et al., Hyperpolarization of deuterated metabolites via remote cross-polarization and dissolution dynamic nuclear polarization. J Phys Chem B, 2014. 118(5): p. 1411-5.
http://www.ncbi.nlm.nih.gov/pubmed/24397585
[NMR paper] Altered transition metal homeostasis in mice following manganese injections for manganese-enhanced magnetic resonance imaging.
Altered transition metal homeostasis in mice following manganese injections for manganese-enhanced magnetic resonance imaging.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Altered transition metal homeostasis in mice following manganese injections for manganese-enhanced magnetic resonance imaging.
Biometals. 2013 Feb;26(1):179-87
Authors: Moldovan N, Al-Ebraheem A, Miksys NA, Farquharson MJ, Bock NA
Abstract
In manganese-enhanced magnetic resonance...
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02-03-2013 10:19 AM
Neurotoxin II Bound to Acetylcholine Receptors in Native Membranes Studied by Dynamic Nuclear Polarization NMR
Neurotoxin II Bound to Acetylcholine Receptors in Native Membranes Studied by Dynamic Nuclear Polarization NMR
Arne H. Linden, Sascha Lange, W. Trent Franks, U?mit Akbey, Edgar Specker, Barth-Jan van Rossum and Hartmut Oschkinat
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja206999c/aop/images/medium/ja-2011-06999c_0003.gif
Journal of the American Chemical Society
DOI: 10.1021/ja206999c
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/4d1LYPHtCFw
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11-11-2011 08:26 AM
Dynamic Nuclear Polarization-Enhanced Solid-State NMR of a 13C-Labeled Signal Peptide Bound to Lipid-Reconstituted Sec Translocon
Dynamic Nuclear Polarization-Enhanced Solid-State NMR of a 13C-Labeled Signal Peptide Bound to Lipid-Reconstituted Sec Translocon
Lenica Reggie, Jakob J. Lopez, Ian Collinson, Clemens Glaubitz and Mark Lorch
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja209378h/aop/images/medium/ja-2011-09378h_0002.gif
Journal of the American Chemical Society
DOI: 10.1021/ja209378h
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/e6Ae3MMc0OU
Dynamic nuclear polarization of nucleic acid with endogenously bound manganese
Linear Mode
