Dynamic nuclear polarization experiments at 14.1 T for solid-state NMR.

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Dynamic nuclear polarization experiments at 14.1 T for solid-state NMR.

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NMR assignment:

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Fragment-based:

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Torsion angles from chemical shifts:

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Promega- Proline

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CSI (via RCI server)

TALOS

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HADDOCK

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Camcoil

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NMR sample preparation:

Protein disorder:

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Protein solubility:

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08-26-2010, 04:41 PM

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Dynamic nuclear polarization experiments at 14.1 T for solid-state NMR.

Dynamic nuclear polarization experiments at 14.1 T for solid-state NMR.

Related Articles Dynamic nuclear polarization experiments at 14.1 T for solid-state NMR.

Phys Chem Chem Phys. 2010 Jun 14;12(22):5799-803

Authors: Matsuki Y, Takahashi H, Ueda K, Idehara T, Ogawa I, Toda M, Akutsu H, Fujiwara T

Instrumentation for high-field dynamic nuclear polarization (DNP) at 14.1 T was developed to enhance the nuclear polarization for NMR of solids. The gyrotron generated 394.5 GHz submillimeter (sub-mm) wave with a power of 40 W in the second harmonic TE(0,6) mode. The sub-mm wave with a power of 0.5-3 W was transmitted to the sample in a low-temperature DNP-NMR probe with a smooth-wall circular waveguide system. The (1)H polarization enhancement factor of up to about 10 was observed for a (13)C-labeled compound with nitroxyl biradical TOTAPOL. The DNP enhancement was confirmed by the static magnetic field dependence of the NMR signal amplitude at 90 K. Improvements of the high-field DNP experiments are discussed.

PMID: 20518128 [PubMed - indexed for MEDLINE]

Source: PubMed

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