Dynamic Equilibria between Monomeric and OligomericMisfolded States of the Mammalian Prion Protein Measured by 19F NMR

		BioNMR > Educational resources > Journal club
Dynamic Equilibria between Monomeric and OligomericMisfolded States of the Mammalian Prion Protein Measured by 19F NMR

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

07-10-2013, 04:12 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,733

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Dynamic Equilibria between Monomeric and OligomericMisfolded States of the Mammalian Prion Protein Measured by 19F NMR

Dynamic Equilibria between Monomeric and OligomericMisfolded States of the Mammalian Prion Protein Measured by 19F NMR

Sacha Thierry Larda, Karen Simonetti, M. Sameer Al-Abdul-Wahid, Simon Sharpe and R. Scott Prosser

Journal of the American Chemical Society
DOI: 10.1021/ja404584s

Source: Journal of the American Chemical Society

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Dynamic equilibria between monomeric and oligomeric misfolded states of the mammalian prion protein measured by (19)F NMR. Dynamic equilibria between monomeric and oligomeric misfolded states of the mammalian prion protein measured by (19)F NMR. Dynamic equilibria between monomeric and oligomeric misfolded states of the mammalian prion protein measured by (19)F NMR. J Am Chem Soc. 2013 Jun 20; Authors: Larda ST, Simonetti K, Al-Abdul-Wahid MS, Sharpe S, Prosser RS Abstract The assembly of misfolded proteins is a critical step in the pathogenesis of amyloid and prion diseases, although the molecular mechanisms underlying this phenomenon are not completely...	nmrlearner	Journal club	0	06-21-2013 01:10 PM
Three-Dimensional Structure of CAP-Gly Domain of Mammalian Dynactin Determined by Magic Angle Spinning NMR Spectroscopy: Conformational Plasticity and Interactions with End Binding Protein EB1 Three-Dimensional Structure of CAP-Gly Domain of Mammalian Dynactin Determined by Magic Angle Spinning NMR Spectroscopy: Conformational Plasticity and Interactions with End Binding Protein EB1 Publication date: Available online 4 May 2013 Source:Journal of Molecular Biology</br> Author(s): Si Yan , Guangjin Hou , Charles D. Schwieters , Shubbir Ahmed , John C. Williams , Tatyana Polenova</br> Microtubules (MTs) and their associated proteins (MAPs) play important roles in vesicle and organelle transport, cell motility and cell division. Perturbation of these...	nmrlearner	Journal club	0	05-04-2013 06:54 AM
Toward the Molecular Basis of Inherited Prion Diseases: NMR Structure of the Human Prion Protein with V210I Mutation. Toward the Molecular Basis of Inherited Prion Diseases: NMR Structure of the Human Prion Protein with V210I Mutation. Toward the Molecular Basis of Inherited Prion Diseases: NMR Structure of the Human Prion Protein with V210I Mutation. J Mol Biol. 2011 Aug 4; Authors: Biljan I, Ilc G, Giachin G, Raspadori A, Zhukov I, Plavec J, Legname G The development of transmissible spongiform encephalopathies (TSEs) is associated with the conversion of the cellular prion protein (PrP(C)) into a misfolded, pathogenic isoform (PrP(Sc)). Spontaneous generation...	nmrlearner	Journal club	0	08-16-2011 01:19 PM
[NMR paper] Water-protein interactions in microcrystalline crh measured by 1H-13C solid-state NMR Water-protein interactions in microcrystalline crh measured by 1H-13C solid-state NMR spectroscopy. Related Articles Water-protein interactions in microcrystalline crh measured by 1H-13C solid-state NMR spectroscopy. J Am Chem Soc. 2003 Nov 5;125(44):13336-7 Authors: Lesage A, Böckmann A Using solid-state NMR carbon-proton dipolar correlation spectroscopy, we observed hydrogen exchange on the millisecond time scale between water molecules and protein protons in a solid sample. These interactions are shown to be related to important structural...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
[NMR paper] Solution NMR study of the monomeric form of p13suc1 protein sheds light on the hinge Solution NMR study of the monomeric form of p13suc1 protein sheds light on the hinge region determining the affinity for a phosphorylated substrate. Related Articles Solution NMR study of the monomeric form of p13suc1 protein sheds light on the hinge region determining the affinity for a phosphorylated substrate. J Biol Chem. 2002 Apr 5;277(14):12375-81 Authors: Odaert B, Landrieu I, Dijkstra K, Schuurman-Wolters G, Casteels P, Wieruszeski JM, Inze D, Scheek R, Lippens G Cyclin-dependent kinase subunit (CKS) proteins bind to cyclin-dependent...	nmrlearner	Journal club	0	11-24-2010 08:49 PM
[NMR paper] Prion protein NMR structure and species barrier for prion diseases. Prion protein NMR structure and species barrier for prion diseases. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-custom-pnas_full_free.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Prion protein NMR structure and species barrier for prion diseases. Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7281-5 Authors: Billeter M, Riek R, Wider G, Hornemann S, Glockshuber R, WÃ¼thrich K The structural...	nmrlearner	Journal club	0	08-22-2010 05:08 PM
[NMR paper] Recognition of DNA by GAL4 in solution: use of a monomeric protein-DNA complex for st Recognition of DNA by GAL4 in solution: use of a monomeric protein-DNA complex for study by NMR. Related Articles Recognition of DNA by GAL4 in solution: use of a monomeric protein-DNA complex for study by NMR. Biochemistry. 1994 Mar 15;33(10):3071-8 Authors: Baleja JD, Mau T, Wagner G The complex of a monomer of GAL4 with DNA has been investigated by two-dimensional 1H nuclear magnetic resonance (NMR) spectroscopy. Previous X-ray analysis has revealed a structure in which a dimer of the N-terminal 65-residue fragment of GAL4 forms a complex,...	nmrlearner	Journal club	0	08-22-2010 03:33 AM
[NMR paper] Recognition of DNA by GAL4 in solution: use of a monomeric protein-DNA complex for st Recognition of DNA by GAL4 in solution: use of a monomeric protein-DNA complex for study by NMR. Related Articles Recognition of DNA by GAL4 in solution: use of a monomeric protein-DNA complex for study by NMR. Biochemistry. 1994 Mar 15;33(10):3071-8 Authors: Baleja JD, Mau T, Wagner G The complex of a monomer of GAL4 with DNA has been investigated by two-dimensional 1H nuclear magnetic resonance (NMR) spectroscopy. Previous X-ray analysis has revealed a structure in which a dimer of the N-terminal 65-residue fragment of GAL4 forms a complex,...	nmrlearner	Journal club	0	08-22-2010 03:33 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off