Publication year: 2012 Source: Journal of Magnetic Resonance, Available online 5 March 2012
Zhengfeng*Zhang, Yimin*Miao, Xiaoli*Liu, Jun*Yang, Conggang*Li, ...
A sinusoidal modulation scheme is described for selective heteronuclear polarization transfer between two dilute spins in double cross polarization magic-angle-spinning nuclear magnetic resonance spectroscopy. During the second N->C cross polarization, theC RF amplitude is modulated sinusoidally while theN RF amplitude is tangent. This modulation induces an effective spin-lock field in two selective frequency bands in either side of theC RF carrier frequency, allowing for simultaneous polarization transfers fromN toC in those two selective frequency bands. It is shown by experiments and simulations that this sinusoidal modulation allows one to selectively polarize fromN to its covalently bondedC? andC’ carbons in neighboring peptide planes simultaneously, which is useful for establishing the backbone connectivity between two sequential residues in protein structural elucidation. The selectivity and efficiency were experimentally demonstrated on a uniformlyC,N-labeled ?1 immunoglobulin binding domain of protein G (GB1). Highlights
? Sine modulation was proposed for dual-band selective DCP experiments. ? Dual-band selective DCP experiments used less RF power for better selectivity. ? Dual-band selective DCP experiments were insensitive to the RF variation. ? NCO / NCA spectra of GB1 were obtained simultaneously in a single experiment.
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nmrlearner
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11-15-2011 10:36 AM
Rapid Solid-State NMR of Deuterated Proteins by Interleaved Cross-Polarization fromH andH Nuclei
Rapid Solid-State NMR of Deuterated Proteins by Interleaved Cross-Polarization fromH andH Nuclei
Publication year: 2011
Source: Journal of Magnetic Resonance, Available online 9 November 2011</br>
Morten*Bjerring, Berit*Paaske, Hartmut*Oschkinat, Umit*Akbey, Niels Chr.*Nielsen</br>
We present a novel sampling strategy, interleaving acquisition of multiple NMR spectra by exploiting initial polarization subsequently fromH andH spins, taking advantage of their differentT1relaxation times. DifferentH- andH-polarization based spectra are in this way simultaneously recorded improving either...
nmrlearner
Journal club
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11-10-2011 07:38 AM
Chemical Shifts for the Unusual DNA Structure in Pf1 Bacteriophage from Dynamic-Nuclear-Polarization-Enhanced Solid-State NMR Spectroscopy.
Chemical Shifts for the Unusual DNA Structure in Pf1 Bacteriophage from Dynamic-Nuclear-Polarization-Enhanced Solid-State NMR Spectroscopy.
Chemical Shifts for the Unusual DNA Structure in Pf1 Bacteriophage from Dynamic-Nuclear-Polarization-Enhanced Solid-State NMR Spectroscopy.
J Am Chem Soc. 2011 Aug 22;
Authors: Sergeyev IV, Day LA, Goldbourt A, McDermott AE
Abstract
Solid state NMR spectra, including dynamic nuclear polarization enhanced 400 MHz spectra acquired at 100K, as well as non-DNP spectra at a variety of field strengths and...
nmrlearner
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08-23-2011 04:03 PM
Ergodicity and efficiency of cross-polarization in NMR of static solids.
Ergodicity and efficiency of cross-polarization in NMR of static solids.
Ergodicity and efficiency of cross-polarization in NMR of static solids.
J Magn Reson. 2011 Apr;209(2):161-6
Authors: Nevzorov AA
Cross-polarization transfer is employed in virtually every solid-state NMR experiment to enhance magnetization of low-gamma spins. Theory and experiment is used to assess the magnitude of the final quasistationary magnetization amplitude. The many-body density matrix equation is solved for relatively large (up to N=14) spin systems without the...
Dynamic nuclear polarization-enhanced solid-state NMR spectroscopy of GNNQQNY nanocry
Dynamic nuclear polarization-enhanced solid-state NMR spectroscopy of GNNQQNY nanocrystals and amyloid fibrils.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles Dynamic nuclear polarization-enhanced solid-state NMR spectroscopy of GNNQQNY nanocrystals and amyloid fibrils.
Phys Chem Chem Phys. 2010 Jun 14;12(22):5911-9
Authors: Debelouchina GT, Bayro MJ, van der Wel PC, Caporini MA, Barnes AB, Rosay M, Maas WE, Griffin RG
Dynamic nuclear polarization (DNP) utilizes the...
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08-26-2010 04:41 PM
Dynamic nuclear polarization experiments at 14.1 T for solid-state NMR.
Dynamic nuclear polarization experiments at 14.1 T for solid-state NMR.
Related Articles Dynamic nuclear polarization experiments at 14.1 T for solid-state NMR.
Phys Chem Chem Phys. 2010 Jun 14;12(22):5799-803
Authors: Matsuki Y, Takahashi H, Ueda K, Idehara T, Ogawa I, Toda M, Akutsu H, Fujiwara T
Instrumentation for high-field dynamic nuclear polarization (DNP) at 14.1 T was developed to enhance the nuclear polarization for NMR of solids. The gyrotron generated 394.5 GHz submillimeter (sub-mm) wave with a power of 40 W in the second harmonic...
nmrlearner
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08-26-2010 04:41 PM
[NMR paper] Polarization transfer by cross-correlated relaxation in solution NMR with very large
Polarization transfer by cross-correlated relaxation in solution NMR with very large molecules.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-custom-pnas_full_free.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Polarization transfer by cross-correlated relaxation in solution NMR with very large molecules.
Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):4918-23
Authors: Riek R, Wider G, Pervushin K, Wüthrich...
Dual-band Selective Double Cross Polarization for Heteronuclear Polarization Transfer between Dilute Spins in Solid-State MAS NMR
Linear Mode
