NMR paper Domain interactions of C-terminal Src Kinase determined through NMR spectroscopy with segmental isotope labeling.

		BioNMR > Educational resources > Journal club
[NMR paper] Domain interactions of C-terminal Src Kinase determined through NMR spectroscopy with segmental isotope labeling.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

01-10-2018, 12:35 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Domain interactions of C-terminal Src Kinase determined through NMR spectroscopy with segmental isotope labeling.

Related Articles Domain interactions of C-terminal Src Kinase determined through NMR spectroscopy with segmental isotope labeling.

Protein Cell. 2017 01;8(1):67-71

Authors: Liu D, Yuan Y, Xu R, Cowburn D

PMID: 27815825 [PubMed - indexed for MEDLINE]

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Efficient segmental isotope labeling of multi-domain proteins using Sortase A Efficient segmental isotope labeling of multi-domain proteins using Sortase A Abstract NMR studies of multi-domain protein complexes provide unique insight into their molecular interactions and dynamics in solution. For large proteins domain-selective isotope labeling is desired to reduce signal overlap, but available methods require extensive optimization and often give poor ligation yields. We present an optimized strategy for segmental labeling of multi-domain proteins using the S. aureus transpeptidase Sortase A. Critical improvements compared to...	nmrlearner	Journal club	0	08-29-2015 09:18 PM
[NMR paper] Unambiguous Assignment of Short- and Long-Range Structural Restraints by Solid-State NMR Spectroscopy with Segmental Isotope Labeling. Unambiguous Assignment of Short- and Long-Range Structural Restraints by Solid-State NMR Spectroscopy with Segmental Isotope Labeling. Related Articles Unambiguous Assignment of Short- and Long-Range Structural Restraints by Solid-State NMR Spectroscopy with Segmental Isotope Labeling. Chembiochem. 2014 Nov 12; Authors: Schubeis T, Lührs T, Ritter C Abstract We present an efficient method for the reduction of spectral complexity in the solid-state NMR spectra of insoluble protein assemblies, without loss of signal intensity....	nmrlearner	Journal club	0	11-14-2014 08:40 PM
Three-Dimensional Structure of CAP-Gly Domain of Mammalian Dynactin Determined by Magic Angle Spinning NMR Spectroscopy: Conformational Plasticity and Interactions with End Binding Protein EB1 Three-Dimensional Structure of CAP-Gly Domain of Mammalian Dynactin Determined by Magic Angle Spinning NMR Spectroscopy: Conformational Plasticity and Interactions with End Binding Protein EB1 Publication date: Available online 4 May 2013 Source:Journal of Molecular Biology</br> Author(s): Si Yan , Guangjin Hou , Charles D. Schwieters , Shubbir Ahmed , John C. Williams , Tatyana Polenova</br> Microtubules (MTs) and their associated proteins (MAPs) play important roles in vesicle and organelle transport, cell motility and cell division. Perturbation of these...	nmrlearner	Journal club	0	05-04-2013 06:54 AM
[NMR paper] Isotope labeling strategies for analysis of an ion channel cytoplasmic domain by NMR spectroscopy. Isotope labeling strategies for analysis of an ion channel cytoplasmic domain by NMR spectroscopy. Related Articles Isotope labeling strategies for analysis of an ion channel cytoplasmic domain by NMR spectroscopy. Methods Mol Biol. 2013;998:289-300 Authors: Abarca-Heidemann K, Duchardt-Ferner E, Woehnert J, Rothberg BS Abstract As large, multimeric, integral membrane proteins, ion channels pose technical challenges to analysis by NMR spectroscopy. Here we present a strategy to overcome some of these technical hurdles, using a...	nmrlearner	Journal club	0	03-27-2013 03:33 PM
[NMR paper] Amino Acid-Selective Segmental Isotope Labeling of Multidomain Proteins for Structural Biology. Amino Acid-Selective Segmental Isotope Labeling of Multidomain Proteins for Structural Biology. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles Amino Acid-Selective Segmental Isotope Labeling of Multidomain Proteins for Structural Biology. Chembiochem. 2013 Jan 30; Authors: Michel E, Skrisovska L, Wüthrich K, Allain FH Abstract Current solution NMR techniques enable structural investigations of proteins in molecular particles with sizes...	nmrlearner	Journal club	0	02-03-2013 10:19 AM
Segmental isotope labeling of proteins for NMR structural study using a protein S tag for higher expression and solubility Segmental isotope labeling of proteins for NMR structural study using a protein S tag for higher expression and solubility Abstract A common obstacle to NMR studies of proteins is sample preparation. In many cases, proteins targeted for NMR studies are poorly expressed and/or expressed in insoluble forms. Here, we describe a novel approach to overcome these problems. In the protein S tag-intein (PSTI) technology, two tandem 92-residue N-terminal domains of protein S (PrS2) from Myxococcus xanthus is fused at the N-terminal end of a protein to enhance its expression and solubility. Using...	nmrlearner	Journal club	0	03-08-2012 08:46 AM
A segmental labeling strategy for unambiguous determination of domainâ??domain interactions of large multi-domain proteins A segmental labeling strategy for unambiguous determination of domainâ??domain interactions of large multi-domain proteins Abstract NMR structural determination of large multi-domain proteins is a challenging task due to significant spectral overlap with a particular difficulty in unambiguous identification of domainâ??domain interactions. Segmental labeling is a NMR strategy that allows for isotopically labeling one domain and leaves the other domain unlabeled. This significantly simplifies spectral overlaps and allows for quick identification of domainâ??domain interaction. Here, a...	nmrlearner	Journal club	0	07-08-2011 07:01 PM
Recent advances in segmental isotope labeling of proteins: NMR applications to large proteins and glycoproteins Recent advances in segmental isotope labeling of proteins: NMR applications to large proteins and glycoproteins Abstract In the last 15 years substantial advances have been made to place isotope labels in native and glycosylated proteins for NMR studies and structure determination. Key developments include segmental isotope labeling using Native Chemical Ligation, Expressed Protein Ligation and Protein Trans-Splicing. These advances are pushing the size limit of NMR spectroscopy further making larger proteins accessible for this technique. It is just emerging that segmental isotope...	nmrlearner	Journal club	0	01-09-2011 12:46 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off