Chromatin is a DNA-protein polymer that represents the functional form of the genome. The main building block of chromatin is the nucleosome, a structure that contains 147 base pairs of DNA and two copies each of the histone proteins H2A, H2B, H3 and H4. Previous work has shown that magic angle spinning (MAS) NMR spectroscopy can capture the nucleosome at high resolution although studies have been challenging due to low sensitivity, the presence of dynamic and rigid components, and the complex...
[NMR paper] Emerging Contributions of Solid-State NMR Spectroscopy to Chromatin Structural Biology
Emerging Contributions of Solid-State NMR Spectroscopy to Chromatin Structural Biology
The eukaryotic genome is packaged into chromatin, a polymer of DNA and histone proteins that regulates gene expression and the spatial organization of nuclear content. The repetitive character of chromatin is diversified into rich layers of complexity that encompass DNA sequence, histone variants and post-translational modifications. Subtle molecular changes in these variables can often lead to global chromatin rearrangements that dictate entire gene programs with far reaching implications for...
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10-29-2021 10:57 AM
Structural Elucidation of Amorphous Photocatalytic Polymers from Dynamic Nuclear Polarization Enhanced Solid State NMR #DNPNMR
From The DNP-NMR Blog:
Structural Elucidation of Amorphous Photocatalytic Polymers from Dynamic Nuclear Polarization Enhanced Solid State NMR #DNPNMR
Brownbill, Nick J., Reiner Sebastian Sprick, Baltasar Bonillo, Shane Pawsey, Fabien Aussenac, Alistair J. Fielding, Andrew I. Cooper, and Frédéric Blanc. “Structural Elucidation of Amorphous Photocatalytic Polymers from Dynamic Nuclear Polarization Enhanced Solid State NMR.” Macromolecules 51, no. 8 (April 24, 2018): 3088–96.
https://doi.org/10.1021/acs.macromol.7b02544.
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11-25-2018 06:02 AM
DNP-enhanced solid-state NMR spectroscopy of active pharmaceutical ingredients #DNPNMR
From The DNP-NMR Blog:
DNP-enhanced solid-state NMR spectroscopy of active pharmaceutical ingredients #DNPNMR
Zhao, L., et al., DNP-enhanced solid-state NMR spectroscopy of active pharmaceutical ingredients. Magn. Reson. Chem., 2017. 0(0).
https://www.ncbi.nlm.nih.gov/pubmed/29193278
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03-26-2018 04:18 PM
Oxygen-17 dynamic nuclear polarisation enhanced solid-state NMR spectroscopy at 18.8 T #DNPNMR
From The DNP-NMR Blog:
Oxygen-17 dynamic nuclear polarisation enhanced solid-state NMR spectroscopy at 18.8 T #DNPNMR
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Brownbill, N.J., et al., Oxygen-17 dynamic nuclear polarisation enhanced solid-state NMR spectroscopy at 18.8 T. Chem Commun (Camb), 2017. 53(17): p. 2563-2566.
https://www.ncbi.nlm.nih.gov/pubmed/28184389
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06-10-2017 05:21 PM
Enlightening the photoactive site of channelrhodopsin-2 by DNP-enhanced solid-state NMR spectroscopy
From The DNP-NMR Blog:
Enlightening the photoactive site of channelrhodopsin-2 by DNP-enhanced solid-state NMR spectroscopy
Becker-Baldus, J., et al., Enlightening the photoactive site of channelrhodopsin-2 by DNP-enhanced solid-state NMR spectroscopy. Proc. Nat. Aca. Sci. USA, 2015.
http://www.pnas.org/content/early/2015/07/23/1507713112.abstract
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08-22-2015 11:20 AM
[NMR paper] Enlightening the photoactive site of channelrhodopsin-2 by DNP-enhanced solid-state NMR spectroscopy.
Enlightening the photoactive site of channelrhodopsin-2 by DNP-enhanced solid-state NMR spectroscopy.
Related Articles Enlightening the photoactive site of channelrhodopsin-2 by DNP-enhanced solid-state NMR spectroscopy.
Proc Natl Acad Sci U S A. 2015 Jul 27;
Authors: Becker-Baldus J, Bamann C, Saxena K, Gustmann H, Brown LJ, Brown RC, Reiter C, Bamberg E, Wachtveitl J, Schwalbe H, Glaubitz C
Abstract
Channelrhodopsin-2 from Chlamydomonas reinhardtii is a light-gated ion channel. Over recent years, this ion channel has...
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07-30-2015 12:01 AM
Improved Structural Elucidation of Synthetic Polymers by Dynamic Nuclear Polarization Solid-State NMR Spectroscopy
From The DNP-NMR Blog:
Improved Structural Elucidation of Synthetic Polymers by Dynamic Nuclear Polarization Solid-State NMR Spectroscopy
Ouari, O., et al., Improved Structural Elucidation of Synthetic Polymers by Dynamic Nuclear Polarization Solid-State NMR Spectroscopy. ACS Macro Letters, 2013. 2(8): p. 715-719.
http://dx.doi.org/10.1021/mz4003003
DNP-enhanced solid-state NMR spectroscopy of chromatin polymers
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