Distinguishing Different Hydrogen-Bonded Helices in Proteins by Efficient 1H-Detected Three-Dimensional Solid-State NMR
 

Distinguishing Different Hydrogen-Bonded Helices in Proteins by Efficient 1H-Detected Three-Dimensional Solid-State NMR

Helical structures in proteins include not only ?-helices but also 3(10) and ? helices. These secondary structures differ in the registry of the C?O···H-N hydrogen bonds, which are i to i + 4 for ?-helices, i to i + 3 for 3(10) helices, and i to i + 5 for ?-helices. The standard NMR observable of protein secondary structures are chemical shifts, which are, however, insensitive to the precise type of helices. Here, we introduce a three-dimensional (3D) ¹H-detected experiment that measures and...

More...