Helical structures in proteins include not only ?-helices but also 3(10) and ? helices. These secondary structures differ in the registry of the C?O···H-N hydrogen bonds, which are i to i + 4 for ?-helices, i to i + 3 for 3(10) helices, and i to i + 5 for ?-helices. The standard NMR observable of protein secondary structures are chemical shifts, which are, however, insensitive to the precise type of helices. Here, we introduce a three-dimensional (3D) ¹H-detected experiment that measures and...
[NMR paper] Characterization of peptide O?HN hydrogen bonds via1H-detected 15N/17O solid-state NMR spectroscopy
Characterization of peptide O?HN hydrogen bonds via1H-detected 15N/17O solid-state NMR spectroscopy
High sensitivity and resolution solid-state NMR methods are reported, that straightforwardly select hydrogen-bonded ^(15)N-^(17)O pairs from amongst all other nitrogen and oxygen sites in peptides, to aid protein secondary and tertiary structure determination. Significantly improved sensitivity is obtained with indirect ¹H detection under fast MAS and stronger relayed dipole couplings.
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02-24-2023 01:13 AM
Efficient solvent suppression with adiabatic inversion for 1H-detected solid-state NMR
Efficient solvent suppression with adiabatic inversion for 1H-detected solid-state NMR
Abstract
This study introduces a conceptually new solvent suppression scheme with adiabatic inversion pulses for 1H-detected multidimensional solid-state NMR (SSNMR) of biomolecules and other systems, which is termed â??Solvent suppression of Liquid signal with Adiabatic Pulseâ?? (SLAP). 1H-detected 2D 13C/1H SSNMR data of uniformly 13C- and 15N-labeled GB1 sample using ultra-fast magic angle spinning at a spinning rate of 60Â*kHz demonstrated that the SLAP scheme...
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10-21-2021 04:58 PM
[NMR paper] Hydrogen bond strength in membrane proteins probed by time-resolved (1)H-detected solid-state NMR and MD simulations.
Hydrogen bond strength in membrane proteins probed by time-resolved (1)H-detected solid-state NMR and MD simulations.
Related Articles Hydrogen bond strength in membrane proteins probed by time-resolved (1)H-detected solid-state NMR and MD simulations.
Solid State Nucl Magn Reson. 2017 Mar 18;:
Authors: Medeiros-Silva J, Jekhmane S, Baldus M, Weingarth M
Abstract
(1)H-detected solid-state NMR in combination with (1)H/(2)D exchange steps allows for the direct identification of very strong hydrogen bonds in membrane proteins....
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03-28-2017 03:06 PM
Hydrogen bond strength in membrane proteins by time-resolved 1H-detected solid-state NMR and MD simulations
Hydrogen bond strength in membrane proteins by time-resolved 1H-detected solid-state NMR and MD simulations
Publication date: Available online 18 March 2017
Source:Solid State Nuclear Magnetic Resonance</br>
Author(s): João Medeiros-Silva, Shehrazade Jekhmane, Marc Baldus, Markus Weingarth</br>
1H-detected solid-state NMR in combination with 1H/2D exchange steps allows for the direct identification of very strong hydrogen bonds in membrane proteins. On the example of the membrane-embedded potassium channel KcsA, we quantify the longevity of such very strong...
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03-19-2017 07:03 AM
[NMR paper] An Efficient Labelling Approach to Harness Backbone and Side-Chain Protons in (1) H-Detected Solid-State NMR Spectroscopy.
An Efficient Labelling Approach to Harness Backbone and Side-Chain Protons in (1) H-Detected Solid-State NMR Spectroscopy.
Related Articles An Efficient Labelling Approach to Harness Backbone and Side-Chain Protons in (1) H-Detected Solid-State NMR Spectroscopy.
Angew Chem Int Ed Engl. 2015 Nov 11;
Authors: Mance D, Sinnige T, Kaplan M, Narasimhan S, Daniëls M, Houben K, Baldus M, Weingarth M
Abstract
(1) H-detection can greatly improve spectral sensitivity in biological solid-state NMR (ssNMR), thus allowing the study of larger...
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11-12-2015 11:28 PM
[NMR paper] Diazole-based powdered cocrystal featuring a helical hydrogen-bonded network: Structure determination from PXRD, solid-state NMR and computer modeling.
Diazole-based powdered cocrystal featuring a helical hydrogen-bonded network: Structure determination from PXRD, solid-state NMR and computer modeling.
Related Articles Diazole-based powdered cocrystal featuring a helical hydrogen-bonded network: Structure determination from PXRD, solid-state NMR and computer modeling.
Solid State Nucl Magn Reson. 2014 Dec 30;
Authors: Sardo M, Santos SM, Babaryk AA, López C, Alkorta I, Elguero J, Claramunt RM, Mafra L
Abstract
We present the structure of a new equimolar 1:1 cocrystal formed by...
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Interaction of epothilone B (patupilone) with microtubules as detected by two-dimensional solid-state NMR spectroscopy.
Interaction of epothilone B (patupilone) with microtubules as detected by two-dimensional solid-state NMR spectroscopy.
Interaction of epothilone B (patupilone) with microtubules as detected by two-dimensional solid-state NMR spectroscopy.
Angew Chem Int Ed Engl. 2010 Oct 4;49(41):7504-7
Authors: Kumar A, Heise H, Blommers MJ, Krastel P, Schmitt E, Petersen F, Jeganathan S, Mandelkow EM, Carlomagno T, Griesinger C, Baldus M
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[NMR paper] Stability of alpha-helices in a molten globule state of cytochrome c by hydrogen-deut
Stability of alpha-helices in a molten globule state of cytochrome c by hydrogen-deuterium exchange and two-dimensional NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Stability of alpha-helices in a molten globule state of cytochrome c by hydrogen-deuterium exchange and two-dimensional NMR spectroscopy.
J Mol Biol. 1995 Apr 7;247(4):682-8
Authors: Kuroda Y, Endo S, Nagayama K, Wada A
To distinguish between intrinsically stable helices and those...
Distinguishing Different Hydrogen-Bonded Helices in Proteins by Efficient 1H-Detected Three-Dimensional Solid-State NMR
Linear Mode
