NMR paper Dissection of heteronuclear NMR experiments for studies of magnetization transfer eff

		BioNMR > Educational resources > Journal club
[NMR paper] Dissection of heteronuclear NMR experiments for studies of magnetization transfer eff

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-24-2010, 09:16 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Dissection of heteronuclear NMR experiments for studies of magnetization transfer eff

Dissection of heteronuclear NMR experiments for studies of magnetization transfer efficiencies.

Related Articles Dissection of heteronuclear NMR experiments for studies of magnetization transfer efficiencies.

J Magn Reson. 2003 Nov;165(1):89-94

Authors: Braun D, Wüthrich K, Wider G

Modern NMR experiments for applications with biological macromolecules in solution typically include multiple magnetization transfer steps. When working with large structures, a significant fraction of the magnetization is lost during these transfers. For the design and optimization of complex experimental schemes, the magnetization transfer efficiencies have therefore commonly been calculated from the spin relaxation times. This paper now suggests a new method for measurement of individual transfer efficiencies directly with the system of interest, using short, reliable experiments. Initial applications of this approach with a 110,000 Da protein indicate that there is a wide range of transfer efficiencies among individual spin pairs in a structure of this size, which leads to a correspondingly large variation of the individual signal intensities and the need for techniques to enhance the weak signals.

PMID: 14568519 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Saturation transfer difference (STD) 1H-NMR experiments and in silico docking experim Saturation transfer difference (STD) 1H-NMR experiments and in silico docking experiments to probe the binding of N-acetylneuraminic acid and derivatives to Vibrio cholerae sialidase. Related Articles Saturation transfer difference (STD) 1H-NMR experiments and in silico docking experiments to probe the binding of N-acetylneuraminic acid and derivatives to Vibrio cholerae sialidase. Proteins. 2004 Aug 1;56(2):346-53 Authors: Haselhorst T, Wilson JC, Thomson RJ, McAtamney S, Menting JG, Coppel RL, von Itzstein M Saturation transfer difference...	nmrlearner	Journal club	0	11-24-2010 10:01 PM
Two-dimensional heteronuclear saturation transfer difference NMR reveals detailed int Two-dimensional heteronuclear saturation transfer difference NMR reveals detailed integrin Î±vÎ²6 protein-peptide interactions. Related Articles Two-dimensional heteronuclear saturation transfer difference NMR reveals detailed integrin Î±vÎ²6 protein-peptide interactions. Chem Commun (Camb). 2010 Sep 13; Authors: Wagstaff JL, Vallath S, Marshall JF, Williamson RA, Howard MJ We report the first example of peptide-protein heteronuclear two-dimensional (2D) saturation transfer difference nuclear magnetic resonance (STD NMR). This method, resulting in...	nmrlearner	Journal club	0	09-15-2010 02:26 PM
[NMR paper] Dissection of the basic subdomain of the c-Jun oncoprotein: a structural analysis of Dissection of the basic subdomain of the c-Jun oncoprotein: a structural analysis of two peptide fragments by CD, Fourier-transform infrared and NMR. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Dissection of the basic subdomain of the c-Jun oncoprotein: a structural analysis of two peptide fragments by CD, Fourier-transform infrared and NMR. Eur J Biochem. 1996 Feb 1;235(3):699-712 Authors: Krebs D, Dahmani B, Monnot M, Mauffret O,...	nmrlearner	Journal club	0	08-22-2010 02:27 PM
[NMR paper] 19F NMR magnetization transfer between 5-FBAPTA and its complexes. An alternative mea 19F NMR magnetization transfer between 5-FBAPTA and its complexes. An alternative means for measuring free Ca2+ concentration, and detection of complexes with protein in erythrocytes. Related Articles 19F NMR magnetization transfer between 5-FBAPTA and its complexes. An alternative means for measuring free Ca2+ concentration, and detection of complexes with protein in erythrocytes. NMR Biomed. 1994 Nov;7(7):330-8 Authors: Gilboa H, Chapman BE, Kuchel PW The 19F NMR Ca(2+)-indicator molecule 5,5'-difluoro-1,2-bis(o-...	nmrlearner	Journal club	0	08-22-2010 03:29 AM
[NMR paper] Secondary structure of human interleukin 2 from 3D heteronuclear NMR experiments. Secondary structure of human interleukin 2 from 3D heteronuclear NMR experiments. Related Articles Secondary structure of human interleukin 2 from 3D heteronuclear NMR experiments. Biochemistry. 1992 Aug 25;31(33):7741-4 Authors: Mott HR, Driscoll PC, Boyd J, Cooke RM, Weir MP, Campbell ID Recombinant 15N-labeled human interleukin 2 (IL-2) has been studied by 2D and 3D NMR using uniformly 15N-labeled protein. Assignment of the backbone resonances has enabled the secondary structure of the protein to be defined. The secondary structure was...	nmrlearner	Journal club	0	08-21-2010 11:45 PM
[NMR paper] Two- and three-dimensional 1H NMR studies of a wheat phospholipid transfer protein: s Two- and three-dimensional 1H NMR studies of a wheat phospholipid transfer protein: sequential resonance assignments and secondary structure. Related Articles Two- and three-dimensional 1H NMR studies of a wheat phospholipid transfer protein: sequential resonance assignments and secondary structure. Biochemistry. 1991 Dec 10;30(49):11600-8 Authors: Simorre JP, Caille A, Marion D, Marion D, Ptak M Two- and three-dimensional 1H NMR experiments have been used to sequentially assign nearly all proton resonances of the 90 residues of wheat...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
Broadband Heteronuclear Solid-State NMR Experiments by Exponentially Modulated Dipola Broadband Heteronuclear Solid-State NMR Experiments by Exponentially Modulated Dipolar Recoupling without Decoupling. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc-MS.gif Related Articles Broadband Heteronuclear Solid-State NMR Experiments by Exponentially Modulated Dipolar Recoupling without Decoupling. J Phys Chem Lett. 2010 Jun 1;1(13):1952-1956 Authors: Nielsen AB, StraasÃ¸ LA, Nieuwkoop AJ, Rienstra CM, Bjerring M, Nielsen NC We present a novel solid-state NMR method for...	nmrlearner	Journal club	0	08-17-2010 03:36 AM
Four-dimensional heteronuclear correlation experiments for chemical shift assignment of solid proteins Four-dimensional heteronuclear correlation experiments for chemical shift assignment of solid proteins W. Trent Franks, Kathryn D. Kloepper, Benjamin J. Wylie and Chad M. Rienstra Journal of Biomolecular NMR; 2007; 39(2); pp 107 - 131 Abstract: Chemical shift assignment is the first step in all established protocols for structure determination of uniformly labeled proteins by NMR. The explosive growth in recent years of magic-angle spinning (MAS) solid-state NMR (SSNMR) applications is largely attributable to improved methods for backbone and side-chain chemical shift correlation...	stewart	Journal club	0	08-05-2008 01:33 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off