The photophysical properties of fluorescent proteins, including phototransformable variants used in advanced microscopy applications, are influenced by the environmental conditions in which they are expressed and used. Rational design of improved fluorescent protein markers requires a better understanding of these environmental effects. We demonstrate here that solution NMR spectroscopy can detect subtle changes in the chemical structure, conformation, and dynamics of the photoactive chromophore...
[ASAP] Disentangling Chromophore States in a Reversibly Switchable Green Fluorescent Protein: Mechanistic Insights from NMR Spectroscopy
Disentangling Chromophore States in a Reversibly Switchable Green Fluorescent Protein: Mechanistic Insights from NMR Spectroscopy
Nina Eleni Christou, Karine Giandoreggio-Barranco, Isabel Ayala, Oleksandr Glushonkov, Virgile Adam, Dominique Bourgeois, and Bernhard Brutscher
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.1c02442/20210509/images/medium/ja1c02442_0007.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.1c02442
http://feeds.feedburner.com/~r/acs/jacsat/~4/sU__ozb5Kl0
[ASAP] Coupling Green Fluorescent Protein Expression with Chemical Modification to Probe Functionally Relevant Riboswitch Conformations in Live Bacteria
Coupling Green Fluorescent Protein Expression with Chemical Modification to Probe Functionally Relevant Riboswitch Conformations in Live Bacteria
https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.8b00316/20180626/images/medium/bi-2018-003163_0005.gif
Biochemistry
DOI: 10.1021/acs.biochem.8b00316
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/Etm4Uq6nj6M
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nmrlearner
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06-27-2018 01:51 AM
Chromophore Isomer Stabilization Is Critical to theEfficient Fluorescence of Cyan Fluorescent Proteins
Chromophore Isomer Stabilization Is Critical to theEfficient Fluorescence of Cyan Fluorescent Proteins
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b01088/20171127/images/medium/bi-2017-01088f_0006.gif
Biochemistry
DOI: 10.1021/acs.biochem.7b01088
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http://feeds.feedburner.com/~r/acs/bichaw/~4/RINOJd7eHmU
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nmrlearner
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11-28-2017 03:58 AM
[NMR paper] Mechanistic Insights into Nanoparticle Surface Adsorption by Solution NMR Spectroscopy in an Aqueous Gel
Mechanistic Insights into Nanoparticle Surface Adsorption by Solution NMR Spectroscopy in an Aqueous Gel
Engineering nanoparticle (NP) functions at molecular level requires a detailed understanding of the dynamic processes occurring at the NP surface. Here we show that the combined analysis of Dark state Exchange Saturation Transfer (DEST) and Relaxation Dispersion (RD) NMR experiments acquired on gel-stabilized samples of NP allows for accurate determination of the kinetics and thermodynamics of adsorption. We used the former approach to describe the interaction of cholic acid (CA) and...
nmrlearner
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06-23-2017 02:24 AM
[NMR paper] A general mechanism of photoconversion of green-to-red fluorescent proteins based on blue and infrared light reduces phototoxicity in live-cell single-molecule imaging
A general mechanism of photoconversion of green-to-red fluorescent proteins based on blue and infrared light reduces phototoxicity in live-cell single-molecule imaging
Photoconversion of fluorescent proteins by blue and complementary near-infrared light, termed primed conversion (PC), is a mechanism recently discovered for Dendra2. We demonstrate that controlling the conformation of arginine at residue 66 by threonine at residue 69 of fluorescent proteins from Anthozoan families (Dendra2, mMaple, Eos, mKikGR, pcDronpa protein families) represents a general route to facilitate PC....
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06-02-2017 08:33 PM
[NMR paper] Characterization of red/green cyanobacteriochrome NpR6012g4 by solution NMR spectroscopy: a hydrophobic pocket for the C15-E,anti chromophore in the photoproduct.
Characterization of red/green cyanobacteriochrome NpR6012g4 by solution NMR spectroscopy: a hydrophobic pocket for the C15-E,anti chromophore in the photoproduct.
Related Articles Characterization of red/green cyanobacteriochrome NpR6012g4 by solution NMR spectroscopy: a hydrophobic pocket for the C15-E,anti chromophore in the photoproduct.
Biochemistry. 2015 May 20;
Authors: Rockwell NC, Martin SS, Lim S, Lagarias JC, Ames JB
Abstract
Cyanobacteriochromes (CBCRs) are cyanobacterial photosensory proteins distantly related to...
Disentangling Chromophore States in a Reversibly Switchable Green Fluorescent Protein: Mechanistic Insights from NMR Spectroscopy
Linear Mode
