Discrimination of human semen specimens by NMR data, sperm parameters, and statistical analysis.
Syst Biol Reprod Med. 2015 Aug 3;:1-7
Authors: Bonechi C, Collodel G, Donati A, Martini S, Moretti E, Rossi C
Abstract
Human seminal fluid is a complex mixture of secretions originated from epididymis and the male accessory sex glands. It contains a variety of both inorganic and organic components, among which proteins are a major part of the high molecular-mass substances. In this study, 83 human seminal plasma samples were analyzed using a combined Nuclear Magnetic Resonance (NMR) Spectroscopy and Principal Component Analysis (PCA) approach to discriminate patients in relation to semen characteristics and/or conditions affecting the fertility status. Results showed a discrimination between patients with leukocytospermia and with the concomitant presence of varicocele/ex varicocele and leukocytospermia. Patients with testicular cancer, necrozoospermia, and azoospermia were separated from the other patient clusters. In addition, a differentiation of semen quality was also possible. This study represents to first use of sperm parameters together with NMR data as variables in the PCA analysis. Furthermore, this methodology allows the identification of the metabolites which play the most important role in identifying differences among human seminal plasma samples.
PMID: 26236922 [PubMed - as supplied by publisher]
[NMR paper] Effects of J couplings and unobservable minor states on kinetics parameters extracted from CEST data
Effects of J couplings and unobservable minor states on kinetics parameters extracted from CEST data
Publication date: Available online 31 October 2014
Source:Journal of Magnetic Resonance</br>
Author(s): Yang Zhou , Daiwen Yang</br>
Chemical Exchange Saturation Transfer (CEST) experiments have emerged as a powerful tool for characterizing dynamics and sparse populated conformers of protein in slow exchanging systems. We show that J couplings and ‘invisible’ minor states can cause systematic errors in kinetics parameters and chemical shifts extracted from CEST...
[NMR paper] Stoichiometric relationship between Na(+) ions transported and glucose consumed in human erythrocytes: Bayesian analysis of (23)Na and (13)C NMR time course data.
Stoichiometric relationship between Na(+) ions transported and glucose consumed in human erythrocytes: Bayesian analysis of (23)Na and (13)C NMR time course data.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-cellhub.gif Related Articles Stoichiometric relationship between Na(+) ions transported and glucose consumed in human erythrocytes: Bayesian analysis of (23)Na and (13)C NMR time course data.
Biophys J. 2013 Apr 16;104(8):1676-84
Authors: Puckeridge M, Chapman BE, Conigrave AD, Grieve SM,...
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[NMR paper] Latent Biochemical Relationships in the Blood-Milk Metabolic Axis of Dairy Cows Revealed by Statistical Integration of (1)H NMR Spectroscopic Data.
Latent Biochemical Relationships in the Blood-Milk Metabolic Axis of Dairy Cows Revealed by Statistical Integration of (1)H NMR Spectroscopic Data.
Latent Biochemical Relationships in the Blood-Milk Metabolic Axis of Dairy Cows Revealed by Statistical Integration of (1)H NMR Spectroscopic Data.
J Proteome Res. 2013 Feb 8;
Authors: Maher AD, Hayes B, Cocks B, Marett L, Wales WJ, Rochfort S
Abstract
A detailed understanding of the relationships between the distinct metabolic compartments of blood and milk would be of potential benefit to...
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[NMR paper] Protein dynamics using frequency-dependent order parameters from analysis of NMR rela
Protein dynamics using frequency-dependent order parameters from analysis of NMR relaxation data.
Related Articles Protein dynamics using frequency-dependent order parameters from analysis of NMR relaxation data.
J Magn Reson. 2003 Mar;161(1):118-25
Authors: Idiyatullin D, Daragan VA, Mayo KH
A novel approach is described to analyze NMR relaxation data on proteins. This method introduces the frequency-dependent order parameter, S(2)(omega), in order to estimate contributions to the generalized order parameter S(2) from different motional...
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[NMR paper] Completeness of NOEs in protein structure: a statistical analysis of NMR.
Completeness of NOEs in protein structure: a statistical analysis of NMR.
Related Articles Completeness of NOEs in protein structure: a statistical analysis of NMR.
J Biomol NMR. 1999 Jun;14(2):123-32
Authors: Doreleijers JF, Raves ML, Rullmann T, Kaptein R
The completeness of experimentally observed NOE restraints of a set of 97 NMR protein structures deposited in the PDB has been assessed. Completeness is defined as the ratio of the number of experimentally observed NOEs and the number of 'expected NOEs'. A practical definition of 'expected...
Discrimination of human semen specimens by NMR data, sperm parameters, and statistical analysis.
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