DirectDetection of the Terminal Hydride Intermediatein [FeFe] Hydrogenase by NMR Spectroscopy

		BioNMR > Educational resources > Journal club
DirectDetection of the Terminal Hydride Intermediatein [FeFe] Hydrogenase by NMR Spectroscopy

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

03-13-2018, 07:39 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

DirectDetection of the Terminal Hydride Intermediatein [FeFe] Hydrogenase by NMR Spectroscopy

DirectDetection of the Terminal Hydride Intermediatein [FeFe] Hydrogenase by NMR Spectroscopy

Sigrun Rumpel, Constanze Sommer, Edward Reijerse, Christophe Fare?s and Wolfgang Lubitz

Journal of the American Chemical Society
DOI: 10.1021/jacs.8b00459

Source: Journal of the American Chemical Society

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
1H NMR Spectroscopy of [FeFe] Hydrogenase:Insight into the Electronic Structure of the Active Site 1H NMR Spectroscopy of Hydrogenase:Insight into the Electronic Structure of the Active Site Sigrun Rumpel, Enrico Ravera, Constanze Sommer, Edward Reijerse, Christophe Fare?s, Claudio Luchinat and Wolfgang Lubitz http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.7b11196/20171214/images/medium/ja-2017-11196m_0004.gif Journal of the American Chemical Society DOI: 10.1021/jacs.7b11196 http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/RSQfNXZbjlI	nmrlearner	Journal club	0	12-15-2017 07:54 AM
[NMR thesis] Pulse NMR in solids : chemical shift, lead fluoride and thorium hydride Pulse NMR in solids : chemical shift, lead fluoride and thorium hydride Lau, Kei-Fung (1976) Pulse NMR in solids : chemical shift, lead fluoride and thorium hydride. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechTHESIS:07172014-132542120 More...	nmrlearner	NMR theses	0	10-01-2017 06:06 AM
Low-Valent Lead Hydride and Its Extreme Low-Field 1H NMR Chemical Shift Low-Valent Lead Hydride and Its Extreme Low-Field 1H NMR Chemical Shift Julia Schneider, Christian P. Sindlinger, Klaus Eichele, Hartmut Schubert and Lars Wesemann http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.7b01856/20170504/images/medium/ja-2017-01856e_0004.gif Journal of the American Chemical Society DOI: 10.1021/jacs.7b01856 http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/8UPLZ8Z2934	nmrlearner	Journal club	0	05-05-2017 09:06 AM
Mechanism of Selective Nickel Transfer from HypB toHypA, Escherichia coli [NiFe]-Hydrogenase AccessoryProteins Mechanism of Selective Nickel Transfer from HypB toHypA, Escherichia coli -Hydrogenase AccessoryProteins http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b00706/20161130/images/medium/bi-2016-00706s_0007.gif Biochemistry DOI: 10.1021/acs.biochem.6b00706 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/Vd4uckPjwj4 More...	nmrlearner	Journal club	0	12-01-2016 12:20 AM
Cross-Species Analysis of Protein Dynamics Associatedwith Hydride and Proton Transfer in the Catalytic Cycle of the Light-DrivenEnzyme Protochlorophyllide Oxidoreductase Cross-Species Analysis of Protein Dynamics Associatedwith Hydride and Proton Transfer in the Catalytic Cycle of the Light-DrivenEnzyme Protochlorophyllide Oxidoreductase http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.5b01355/20160203/images/medium/bi-2015-01355h_0006.gif Biochemistry DOI: 10.1021/acs.biochem.5b01355 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/ajQwVlw7604 More...	nmrlearner	Journal club	0	02-04-2016 11:46 AM
[NMR thesis] Pulse NMR in solids : chemical shift, lead fluoride and thorium hydride Pulse NMR in solids : chemical shift, lead fluoride and thorium hydride Lau, Kei-Fung (1976) Pulse NMR in solids : chemical shift, lead fluoride and thorium hydride. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechTHESIS:07172014-132542120 More...	nmrlearner	NMR theses	0	07-19-2014 01:03 AM
[NMR paper] 1H NMR spectroscopy reveals that mouse Hsp25 has a flexible C-terminal extension of 1 1H NMR spectroscopy reveals that mouse Hsp25 has a flexible C-terminal extension of 18 amino acids. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles 1H NMR spectroscopy reveals that mouse Hsp25 has a flexible C-terminal extension of 18 amino acids. FEBS Lett. 1995 Aug 7;369(2-3):305-10 Authors: Carver JA, Esposito G, Schwedersky G, Gaestel M The small heat-shock proteins (Hsps) exist as large aggregates and function by interacting and stabilising non-native proteins in...	nmrlearner	Journal club	0	08-22-2010 03:50 AM
[NMR paper] The importance of binding energy in catalysis of hydride transfer by UDP-galactose 4- The importance of binding energy in catalysis of hydride transfer by UDP-galactose 4-epimerase: a 13C and 15N NMR and kinetic study. Related Articles The importance of binding energy in catalysis of hydride transfer by UDP-galactose 4-epimerase: a 13C and 15N NMR and kinetic study. Biochemistry. 1993 Dec 7;32(48):13220-30 Authors: Burke JR, Frey PA UDP-galactose 4-epimerase contains NAD+ irreversibly but noncovalently bound to the active site. Uridine nucleotides bind to the substrate site and induce a protein conformational change that...	nmrlearner	Journal club	0	08-22-2010 03:01 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off