The intracellular milieu is crowded and heterogeneous, and this can have profound consequences for biomolecule motions and biochemical kinetics. Macromolecular crowding has been traditionally studied in artificial crowders like Ficoll and dextran or globular proteins such as bovine serum albumin. It is, however, not clear if the effects of artificial crowders on such phenomena are the same as the crowding that is experienced in a heterogeneous biological environment. Bacterial cells, for...
[NMR paper] Fluorine NMR spectroscopy enables to quantify the affinity between DNA and proteins in cell lysate
Fluorine NMR spectroscopy enables to quantify the affinity between DNA and proteins in cell lysate
The determination of the binding affinity quantifying the interaction between proteins and nucleic acids is of crucial interest in biological and chemical research. Here, we have made use of sitespecific fluorine labeling of the cold shock protein from Bacillus subtiliis , Bs CspB, enabling to directly monitor the interaction with single stranded DNA molecules in cell lysate. High-resolution 19 F NMR spectroscopy has been applied to exclusively report on resonance signals arising from the......
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08-14-2021 11:29 PM
[NMR paper] Insights into protein stability in cell lysate by 19F NMR spectroscopy.
Insights into protein stability in cell lysate by 19F NMR spectroscopy.
Related Articles Insights into protein stability in cell lysate by 19F NMR spectroscopy.
Chembiochem. 2020 Aug 12;:
Authors: Welte H, Kovermann M
Abstract
In living organisms, protein folding and function take place in an inhomogeneous, highly crowded environment possessing a concentration of diverse macromolecules of up to 400*g/L. It has been shown that the intracellular environment has a pronounced effect on the stability, dynamics and function of the...
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08-15-2020 05:51 AM
[NMR paper] Combining Diffusion NMR and Small-Angle Neutron Scattering Enables Precise Measurements of Polymer Chain Compression in a Crowded Environment.
Combining Diffusion NMR and Small-Angle Neutron Scattering Enables Precise Measurements of Polymer Chain Compression in a Crowded Environment.
Related Articles Combining Diffusion NMR and Small-Angle Neutron Scattering Enables Precise Measurements of Polymer Chain Compression in a Crowded Environment.
Phys Rev Lett. 2017 Mar 03;118(9):097801
Authors: Palit S, He L, Hamilton WA, Yethiraj A, Yethiraj A
Abstract
The effect of particles on the behavior of polymers in solution is important in a number of important phenomena such as...
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03-19-2017 10:38 PM
[NMR paper] Bacterial cell wall composition and the influence of antibiotics by cell-wall and whole-cell NMR.
Bacterial cell wall composition and the influence of antibiotics by cell-wall and whole-cell NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-roysocjnls_full.gif Related Articles Bacterial cell wall composition and the influence of antibiotics by cell-wall and whole-cell NMR.
Philos Trans R Soc Lond B Biol Sci. 2015 Oct 5;370(1679)
Authors: Romaniuk JA, Cegelski L
Abstract
The ability to characterize bacterial cell-wall composition and structure is...
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07-07-2016 05:58 PM
[NMR paper] Spectral snapshots of bacterial cell-wall composition and the influence of antibiotics by whole-cell NMR.
Spectral snapshots of bacterial cell-wall composition and the influence of antibiotics by whole-cell NMR.
Spectral snapshots of bacterial cell-wall composition and the influence of antibiotics by whole-cell NMR.
Biophys J. 2015 Mar 24;108(6):1380-9
Authors: Nygaard R, Romaniuk JA, Rice DM, Cegelski L
Abstract
Gram-positive bacteria surround themselves with a thick cell wall that is essential to cell survival and is a major target of antibiotics. Quantifying alterations in cell-wall composition are crucial to evaluating drug...
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03-27-2015 11:59 PM
[NMR paper] A 2H solid-state NMR study of lipid clustering by cationic antimicrobial and cell-penetrating peptides in model bacterial membranes.
A 2H solid-state NMR study of lipid clustering by cationic antimicrobial and cell-penetrating peptides in model bacterial membranes.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-cellhub.gif Related Articles A 2H solid-state NMR study of lipid clustering by cationic antimicrobial and cell-penetrating peptides in model bacterial membranes.
Biophys J. 2013 Nov 19;105(10):2333-42
Authors: Kwon B, Waring AJ, Hong M
Abstract
Domain formation in bacteria-mimetic membranes...
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07-12-2014 04:28 AM
[NMR paper] 1H NMR Self-Diffusion in Polymer-Surfactant Nanocapsules and Cryogels with Enzyme.
1H NMR Self-Diffusion in Polymer-Surfactant Nanocapsules and Cryogels with Enzyme.
Related Articles 1H NMR Self-Diffusion in Polymer-Surfactant Nanocapsules and Cryogels with Enzyme.
J Colloid Interface Sci. 1998 Oct 1;206(1):168-176
Authors: Shapiro YE, Pykhteeva EG, Levashov AV
The multicomponent self-diffusion in nanocapsules and cryogel biocatalytic systems containing alpha-chymotrypsin has been studied with the NMR-PGSE method at various temperatures and compared with the diffusion of such systems without enzyme. Unilamellar vesicles have...
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11-17-2010 11:15 PM
Self-diffusion in Polymer Systems studied by Magnetic Field-Gradient Spin-Echo NMR Me
Self-diffusion in Polymer Systems studied by Magnetic Field-Gradient Spin-Echo NMR Methods
Publication year: 2010
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 13 April 2010</br>
Harald, Walderhaug , Olle, Söderman , Daniel, Topgaard</br>
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Diffusion NMR and Rheology of a Model Polymer in Bacterial Cell Lysate Crowders
Linear Mode
