Related ArticlesDevelopment of NMR instrumentation to achieve excitation of large bandwidths in high-resolution spectra at high field.
J Magn Reson. 2001 Jun;150(2):161-6
Authors: Luchinat C, Piccioli M, Pierattelli R, Engelke F, Marquardsen T, Ruin R
A prototype 2.5-mm (1)H high-resolution probe for an 18.8-T (800 MHz) nuclear magnetic resonance spectrometer has been designed, together with a dedicated amplifier capable of delivering up to 1 kW of power. This probe permits a 90 degrees pulse length of 2 mus to be achieved at 300 W, corresponding to an excitation bandwidth of +/-125 kHz. Probe performances were tested on samples commonly used for this purpose as well as on protein and paramagnetic model compound samples. It is shown that this probe is useful for a wide range of applications at high magnetic field, especially in the study of systems characterized by very broad and far-shifted resonances and in experiments that require high-power radiofrequency irradiation.
Three-dimensional deuterium-carbon correlation experiments for high-resolution solid-state MAS NMR spectroscopy of large proteins
Three-dimensional deuterium-carbon correlation experiments for high-resolution solid-state MAS NMR spectroscopy of large proteins
Abstract Well-resolved 2Hâ??13C correlation spectra, reminiscent of 1Hâ??13C correlations, are obtained for perdeuterated ubiquitin and for perdeuterated outer-membrane protein G (OmpG) from E. coli by exploiting the favorable lifetime of 2H double-quantum (DQ) states. Sufficient signal-to-noise was achieved due to the short deuterium T 1, allowing for high repetition rates and enabling 3D experiments with a 2Hâ??13C transfer step in a reasonable time....
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11-01-2011 01:52 AM
Director of Research Instrumentation | University of Nebraska
Director of Research Instrumentation | University of Nebraska
US - NE - Lincoln, The Department of Chemistry seeks candidates for theposition of NMR specialist and Director of the Research InstrumentationFacility. The position will be a full-time, non-tenure-leading Research
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06-06-2011 12:53 AM
[NMR paper] Towards high-resolution solid-state NMR on large uniformly 15N- and [13C,15N]-labeled
Towards high-resolution solid-state NMR on large uniformly 15N- and -labeled membrane proteins in oriented lipid bilayers.
Related Articles Towards high-resolution solid-state NMR on large uniformly 15N- and -labeled membrane proteins in oriented lipid bilayers.
J Biomol NMR. 2002 Mar;22(3):225-47
Authors: Vosegaard T, Nielsen NC
Based on exact numerical simulations, taking into account isotropic and conformation-dependent anisotropic nuclear spin interactions, we systematically analyse the prospects for high-resolution solid-state NMR on...
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11-24-2010 08:49 PM
[NMR paper] Improved excitation pulse bandwidths using shaped pulses, with application to heteron
Improved excitation pulse bandwidths using shaped pulses, with application to heteronuclear half filters in macromolecular NMR.
Related Articles Improved excitation pulse bandwidths using shaped pulses, with application to heteronuclear half filters in macromolecular NMR.
J Magn Reson B. 1995 Jul;108(1):12-21
Authors: Hyre DE, Spicer LD
The advantageous use of sinc-shaped pulses in heteronuclear half filters is explored for studying biological macromolecules. The typical square, or hard, pulse used in half-filter pulse sequences for...
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