Deuterium (²H) spin relaxation of ^(13)CH(2)D methyl groups has been widely applied to investigate picosecond-to-nanosecond conformational dynamics in proteins by solution-state NMR spectroscopy. The B(0) dependence of the ²H spin relaxation rates is represented by a linear relationship between the spectral density function at three discrete frequencies J(0), J(?(D)) and J(2?(D)). In this study, the linear relation between ²H relaxation rates at B(0) fields separated by a factor of two and the...
Electron spin resonance studies on deuterated nitroxyl spin probes used in Overhauser-enhanced magnetic resonance imaging #DNPNMR #ODNP
From The DNP-NMR Blog:
Electron spin resonance studies on deuterated nitroxyl spin probes used in Overhauser-enhanced magnetic resonance imaging #DNPNMR #ODNP
Jebaraj, D. David, Hideo Utsumi, and A. Milton Franklin Benial. “Electron Spin Resonance Studies on Deuterated Nitroxyl Spin Probes Used in Overhauser-Enhanced Magnetic Resonance Imaging.” Magnetic Resonance in Chemistry 55, no. 8 (2017): 700–705.
https://doi.org/10.1002/mrc.4576.
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06-23-2018 12:47 AM
[NMR paper] Enhanced Spectral Density Mapping through Combined Multiple-Field Deuterium mCH2D Methyl Spin Relaxation NMR Spectroscopy.
Enhanced Spectral Density Mapping through Combined Multiple-Field Deuterium mCH2D Methyl Spin Relaxation NMR Spectroscopy.
Related Articles Enhanced Spectral Density Mapping through Combined Multiple-Field Deuterium mCH2D Methyl Spin Relaxation NMR Spectroscopy.
Methods. 2017 Dec 27;:
Authors: Hsu A, O'Brien PA, Bhattacharya S, Rance M, Palmer AG
Abstract
Quadrupolar relaxation of 2H (D) nuclear spins is a powerful probe of conformational dynamics in biological macromolecules. Deuterium relaxation rate constants are determined by...
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01-01-2018 02:17 AM
Dynamic nuclear polarization studies on deuterated nitroxyl spin probes #DNPNMR
From The DNP-NMR Blog:
Dynamic nuclear polarization studies on deuterated nitroxyl spin probes #DNPNMR
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David Jebaraj, D., H. Utsumi, and A. Milton Franklin Benial, Dynamic nuclear polarization studies on deuterated nitroxyl spin probes. Magn Reson Chem, 2017. 55(10): p. 909-916.
https://www.ncbi.nlm.nih.gov/pubmed/28444914
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10-03-2017 03:24 AM
Partially-deuterated samples of HET-s(218â??289) fibrils: assignment and deuterium isotope effect
Partially-deuterated samples of HET-s(218â??289) fibrils: assignment and deuterium isotope effect
Abstract
Fast magic-angle spinning and partial sample deuteration allows direct detection of 1H in solid-state NMR, yielding significant gains in mass sensitivity. In order to further analyze the spectra, 1H detection requires assignment of the 1H resonances. In this work, resonance assignments of backbone HN and Hα are presented for HET-s(218â??289) fibrils, based on the existing assignment of Cα, Cβ, Câ??, and N resonances. The samples used are...
[NMR paper] Mapping oxygen accessibility to ribonuclease a using high-resolution NMR relaxation s
Mapping oxygen accessibility to ribonuclease a using high-resolution NMR relaxation spectroscopy.
Related Articles Mapping oxygen accessibility to ribonuclease a using high-resolution NMR relaxation spectroscopy.
Biophys J. 2004 Mar;86(3):1713-25
Authors: Teng CL, Bryant RG
Paramagnetic contributions to nuclear magnetic spin-lattice relaxation rate constant induced by freely diffusing molecular oxygen measured at hundreds of different protein proton sites provide a direct means for characterizing the exploration of the protein by oxygen. This...
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11-24-2010 09:25 PM
[NMR paper] Backbone dynamics of the cytotoxic ribonuclease alpha-sarcin by 15N NMR relaxation me
Backbone dynamics of the cytotoxic ribonuclease alpha-sarcin by 15N NMR relaxation methods.
Related Articles Backbone dynamics of the cytotoxic ribonuclease alpha-sarcin by 15N NMR relaxation methods.
J Biomol NMR. 2002 Dec;24(4):301-16
Authors: Pérez-Cañadillas JM, Guenneugues M, Campos-Olivas R, Santoro J, Martínez del Pozo A, Gavilanes JG, Rico M, Bruix M
The cytotoxic ribonuclease alpha-sarcin is a 150-residue protein that inactivates ribosomes by selectively cleaving a single phosphodiester bond in a strictly conserved rRNA loop. In order...
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11-24-2010 08:58 PM
[NMR paper] Investigation of ribonuclease T1 folding intermediates by hydrogen-deuterium amide ex
Investigation of ribonuclease T1 folding intermediates by hydrogen-deuterium amide exchange-two-dimensional NMR spectroscopy.
Related Articles Investigation of ribonuclease T1 folding intermediates by hydrogen-deuterium amide exchange-two-dimensional NMR spectroscopy.
Biochemistry. 1993 Jun 22;32(24):6152-6
Authors: Mullins LS, Pace CN, Raushel FM
The rate of hydrogen bond formation at individual amino acid residues in ribonuclease T1 (RNase T1) has been investigated by the hydrogen-deuterium exchange-2D NMR (HDEx-2D NMR) technique (Udgaonkar...
Deuterium spin relaxation of fractionally deuterated ribonuclease H using paired 475 and 950 MHz NMR spectrometers
Linear Mode
