NMR paper Deuterium solid-state NMR quadrupolar order rotating frame relaxation with applications to amyloid-? fibrils.

		BioNMR > Educational resources > Journal club
[NMR paper] Deuterium solid-state NMR quadrupolar order rotating frame relaxation with applications to amyloid-? fibrils.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-11-2020, 09:42 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Deuterium solid-state NMR quadrupolar order rotating frame relaxation with applications to amyloid-? fibrils.

Deuterium solid-state NMR quadrupolar order rotating frame relaxation with applications to amyloid-? fibrils.

Related Articles Deuterium solid-state NMR quadrupolar order rotating frame relaxation with applications to amyloid-? fibrils.

Magn Reson Chem. 2020 Nov 08;:

Authors: Vugmeyster L, Ostrovsky D

Abstract
We describe a new method for measuring molecular dynamics based on the deuterium solid-state nuclear magnetic resonance (NMR) quadrupolar order rotating frame relaxation rate R1?,Q under static conditions. The observed quadrupolar order coherence is created using the broad-band Jeener-Broekaert excitation and is locked with a weak radio frequency (RF) field. We describe the experimental and theoretical approaches and show applications to a selectively deuterated valine side chain of the phosphorylated amyloid-? (1-40) fibrils phosphorylated at the serine-8 position. The R1?,Q rate is sensitive to the rotameric exchange mode. For biological samples, the low spin-lock field in the 5- to 10-kHz range has the advantage of avoiding sample heating and dehydration. Thus, it provides an alternative to approaches based on single-quantum coherence, which require larger spin-lock fields.

PMID: 33161607 [PubMed - as supplied by publisher]

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
New Methodsand Applications in Solid-State NMR Spectroscopyof Quadrupolar Nuclei New Methodsand Applications in Solid-State NMR Spectroscopyof Quadrupolar Nuclei Sharon E. Ashbrook and Scott Sneddon http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja504734p/20141021/images/medium/ja-2014-04734p_0012.gif Journal of the American Chemical Society DOI: 10.1021/ja504734p http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/74mZ1lkq7ug	nmrlearner	Journal club	0	10-21-2014 11:31 PM
[NMR paper] Slow motions in microcrystalline proteins as observed by MAS-dependent (15)N rotating-frame NMR relaxation. Slow motions in microcrystalline proteins as observed by MAS-dependent (15)N rotating-frame NMR relaxation. Related Articles Slow motions in microcrystalline proteins as observed by MAS-dependent (15)N rotating-frame NMR relaxation. J Magn Reson. 2014 Sep 20;248C:8-12 Authors: Krushelnitsky A, Zinkevich T, Reif B, Saalwächter K Abstract (15)N NMR relaxation rate R1? measurements reveal that a substantial fraction of residues in the microcrystalline chicken alpha-spectrin SH3 domain protein undergoes dynamics in the ?s-ms...	nmrlearner	Journal club	0	10-06-2014 12:37 PM
[NMR paper] Slow motions in microcrystalline proteins as observed by MAS-dependent 15N rotating-frame NMR relaxation Slow motions in microcrystalline proteins as observed by MAS-dependent 15N rotating-frame NMR relaxation Publication date: Available online 20 September 2014 Source:Journal of Magnetic Resonance</br> Author(s): Alexey Krushelnitsky , Tatiana Zinkevich , Bernd Reif , Kay Saalwächter</br> 15N NMR relaxation rate R 1? measurements reveal that a substantial fraction of residues in the microcrystalline chicken alpha-spectrin SH3 domain protein undergoes dynamics in the ?s - ms timescale range. On the basis of a comparison of 2D site-resolved with 1D integrated 15N...	nmrlearner	Journal club	0	09-20-2014 07:51 PM
Off-resonance rotating-frame relaxation dispersion experiment for 13C in aromatic side chains using L-optimized TROSY-selection Off-resonance rotating-frame relaxation dispersion experiment for 13C in aromatic side chains using L-optimized TROSY-selection Abstract Protein dynamics on the microsecondâ??millisecond time scales often play a critical role in biological function. NMR relaxation dispersion experiments are powerful approaches for investigating biologically relevant dynamics with site-specific resolution, as shown by a growing number of publications on enzyme catalysis, protein folding, ligand binding, and allostery. To date, the majority of studies has probed the...	nmrlearner	Journal club	0	06-19-2014 10:21 PM
Hydration water dynamics in biopolymers from NMR relaxation in the rotating frame. Hydration water dynamics in biopolymers from NMR relaxation in the rotating frame. Related Articles Hydration water dynamics in biopolymers from NMR relaxation in the rotating frame. J Magn Reson. 2010 Sep 24; Authors: Blicharska B, Peemoeller H, Witek M Assuming dipole-dipole interaction as the dominant relaxation mechanism of protons of water molecules adsorbed onto macromolecule (biopolymer) surfaces we have been able to model the dependences of relaxation rates on temperature and frequency. For adsorbed water molecules the correlation times are...	nmrlearner	Journal club	0	10-22-2010 04:33 PM
[NMR paper] Relaxation-matrix formalism for rotating-frame spin-lattice proton NMR relaxation and Relaxation-matrix formalism for rotating-frame spin-lattice proton NMR relaxation and magnetization transfer in the presence of an off-resonance irradiation field. Related Articles Relaxation-matrix formalism for rotating-frame spin-lattice proton NMR relaxation and magnetization transfer in the presence of an off-resonance irradiation field. J Magn Reson B. 1994 May;104(1):11-25 Authors: Kuwata K, Brooks D, Yang H, Schleich T The derivation of a generalized relaxation matrix equation for the off-resonance rotating-frame spin-lattice...	nmrlearner	Journal club	0	08-22-2010 03:33 AM
[NMR paper] Relaxation-matrix formalism for rotating-frame spin-lattice proton NMR relaxation and Relaxation-matrix formalism for rotating-frame spin-lattice proton NMR relaxation and magnetization transfer in the presence of an off-resonance irradiation field. Related Articles Relaxation-matrix formalism for rotating-frame spin-lattice proton NMR relaxation and magnetization transfer in the presence of an off-resonance irradiation field. J Magn Reson B. 1994 May;104(1):11-25 Authors: Kuwata K, Brooks D, Yang H, Schleich T The derivation of a generalized relaxation matrix equation for the off-resonance rotating-frame spin-lattice...	nmrlearner	Journal club	0	08-22-2010 03:33 AM
[NMR paper] Off-resonance rotating frame spin-lattice NMR relaxation studies of phosphorus metabo Off-resonance rotating frame spin-lattice NMR relaxation studies of phosphorus metabolite rotational diffusion in bovine lens homogenates. Related Articles Off-resonance rotating frame spin-lattice NMR relaxation studies of phosphorus metabolite rotational diffusion in bovine lens homogenates. Biochemistry. 1990 Aug 21;29(33):7547-57 Authors: Caines GH, Schleich T, Morgan CF, Farnsworth PN The rotational diffusion behavior of phosphorus metabolites present in calf lens cortical and nuclear homogenates was investigated by the NMR technique of...	nmrlearner	Journal club	0	08-21-2010 11:04 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off