Deuterium Magic Angle Spinning NMR Used to Study the Dynamics of Peptides Adsorbed onto Polystyrene and Functionalized Polystyrene Surfaces.
J Phys Chem B. 2011 Jun 8;
Authors: Breen NF, Li K, Olsen GL, Drobny GP
LK?14 is a 14 amino acid peptide which displays a periodic alternation of leucine and lysine amino acids. This "hydrophobic periodicity" has been found to result in an ?-helical secondary structure at air-water interfaces and on both polar and non-polar solid polymer surfaces. In this paper the dynamics of LK?14 peptides, selectively deuterated at a single leucine and adsorbed onto polystyrene and carboxylated polystyrene beads, are studied using 2H Magic Angle Spinning (MAS) solid state NMR over a 100 degree temperature range. We first demonstrate the sensitivity enhancement possible with 2H MAS techniques, which in turn enables us to obtain high quality 2H NMR spectra for selectively deuterated peptides adsorbed onto solid polymer surfaces. An extensive literature shows that the dynamics of leucine side chains are sensitive to the local structural environment of the protein. Therefore the degree to which the dynamics of leucine side chains and the backbone of the peptide LK?14 are influenced by surface proximity and surface chemistry is studied as a function of temperature with 2H MAS NMR. It is found that the dynamics of the leucine side chains in LK?14 depend strongly upon the orientation of the polymer on the surface, which in turn depends on whether the LK?14 peptide adsorbs onto a polar or non-polar surface. 2H MAS line shapes therefore permit probes of surface orientation over a wide temperature range.
PMID: 21650191 [PubMed - as supplied by publisher]
Protonation, Tautomerization, and Rotameric Structure of Histidine: A Comprehensive Study by Magic-Angle-Spinning Solid-State NMR.
Protonation, Tautomerization, and Rotameric Structure of Histidine: A Comprehensive Study by Magic-Angle-Spinning Solid-State NMR.
Protonation, Tautomerization, and Rotameric Structure of Histidine: A Comprehensive Study by Magic-Angle-Spinning Solid-State NMR.
J Am Chem Soc. 2011 Jan 5;
Authors: Li S, Hong M
Histidine structure and chemistry lie at the heart of many enzyme active sites, ion channels, and metalloproteins. While solid-state NMR spectroscopy has been used to study histidine chemical shifts, the full pH dependence of the...
nmrlearner
Journal club
0
01-07-2011 11:21 PM
Protonation, Tautomerization, and Rotameric Structure of Histidine: A Comprehensive Study by Magic-Angle-Spinning Solid-State NMR
Protonation, Tautomerization, and Rotameric Structure of Histidine: A Comprehensive Study by Magic-Angle-Spinning Solid-State NMR
Shenhui Li and Mei Hong
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja108943n/aop/images/medium/ja-2010-08943n_0010.gif
Journal of the American Chemical Society
DOI: 10.1021/ja108943n
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/FuFM0C9qHyE
nmrlearner
Journal club
0
01-05-2011 11:40 PM
[NMR paper] Determination of membrane protein structure and dynamics by magic-angle-spinning solid-state NMR spectroscopy.
Determination of membrane protein structure and dynamics by magic-angle-spinning solid-state NMR spectroscopy.
Related Articles Determination of membrane protein structure and dynamics by magic-angle-spinning solid-state NMR spectroscopy.
J Am Chem Soc. 2005 Sep 21;127(37):12965-74
Authors: Andronesi OC, Becker S, Seidel K, Heise H, Young HS, Baldus M
It is shown that molecular structure and dynamics of a uniformly labeled membrane protein can be studied under magic-angle-spinning conditions. For this purpose, dipolar recoupling experiments...
nmrlearner
Journal club
0
12-01-2010 06:56 PM
[NMR paper] 13C magic angle spinning NMR evidence for a 15,15'-cis configuration of the spheroide
13C magic angle spinning NMR evidence for a 15,15'-cis configuration of the spheroidene in the Rhodobacter sphaeroides photosynthetic reaction center.
Related Articles 13C magic angle spinning NMR evidence for a 15,15'-cis configuration of the spheroidene in the Rhodobacter sphaeroides photosynthetic reaction center.
Biochemistry. 1992 Dec 15;31(49):12446-50
Authors: de Groot HJ, Gebhard R, van der Hoef I, Hoff AJ, Lugtenburg J, Violette CA, Frank HA
The photosynthetic reaction center of Rhodobacter sphaeroides 2.4.1 contains one carotenoid...
nmrlearner
Journal club
0
08-21-2010 11:45 PM
[NMR paper] 13C magic angle spinning NMR study of the light-induced and temperature-dependent cha
13C magic angle spinning NMR study of the light-induced and temperature-dependent changes in Rhodobacter sphaeroides R26 reaction centers enriched in tyrosine.
Related Articles 13C magic angle spinning NMR study of the light-induced and temperature-dependent changes in Rhodobacter sphaeroides R26 reaction centers enriched in tyrosine.
Biochemistry. 1992 Nov 17;31(45):11038-49
Authors: Fischer MR, de Groot HJ, Raap J, Winkel C, Hoff AJ, Lugtenburg J
Solid-state 13C magic angle spinning (MAS) NMR has been used to investigate...
nmrlearner
Journal club
0
08-21-2010 11:45 PM
[NMR paper] 13C magic-angle spinning NMR studies of bathorhodopsin, the primary photoproduct of r
13C magic-angle spinning NMR studies of bathorhodopsin, the primary photoproduct of rhodopsin.
Related Articles 13C magic-angle spinning NMR studies of bathorhodopsin, the primary photoproduct of rhodopsin.
Biochemistry. 1991 Jul 30;30(30):7409-15
Authors: Smith SO, Courtin J, de Groot H, Gebhard R, Lugtenburg J
Magic-angle spinning NMR spectra have been obtained of the bathorhodopsin photointermediate trapped at low temperature (less than 130 K) by using isorhodopsin samples regenerated with retinal specifically 13C-labeled at positions 8,...
nmrlearner
Journal club
0
08-21-2010 11:12 PM
[NMR paper] 13C magic-angle spinning NMR studies of bathorhodopsin, the primary photoproduct of r
13C magic-angle spinning NMR studies of bathorhodopsin, the primary photoproduct of rhodopsin.
Related Articles 13C magic-angle spinning NMR studies of bathorhodopsin, the primary photoproduct of rhodopsin.
Biochemistry. 1991 Jul 30;30(30):7409-15
Authors: Smith SO, Courtin J, de Groot H, Gebhard R, Lugtenburg J
Magic-angle spinning NMR spectra have been obtained of the bathorhodopsin photointermediate trapped at low temperature (less than 130 K) by using isorhodopsin samples regenerated with retinal specifically 13C-labeled at positions 8,...