Liquid-Liquid phase separation has emerged as fundamental process underlying the formation of biomolecular condensates. Insights into the composition and structure of biomolecular condensates is, however, complicated by their molecular complexity and dynamics. Here, we introduce an improved spatially-resolved NMR experiment that enables quantitative analysis of the physico-chemical composition of multi-component biomolecular condensates in equilibrium and label-free. Application of...
[NMR paper] (15)N NMR studies provide insights into physico-chemical properties of room-temperature ionic liquids
(15)N NMR studies provide insights into physico-chemical properties of room-temperature ionic liquids
Ionic liquids (ILs) have gained a lot of attention as alternative solvents in many fields of science in the last two decades. It is known that the type of anion has a significant influence on the macroscopic properties of the IL. To gain insights into the molecular mechanisms responsible for these effects it is important to characterize these systems at the microscopic level. Such information can be obtained from nuclear spin-relaxation studies which for compounds with natural isotope...
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05-24-2021 11:59 PM
Spatially resolved NMR spectroscopy of heterogeneous gas phase hydrogenation of 1,3-butadiene with para-hydrogen
From The DNP-NMR Blog:
Spatially resolved NMR spectroscopy of heterogeneous gas phase hydrogenation of 1,3-butadiene with para-hydrogen
Svyatova, Alexandra, Elizaveta S. Kononenko, Kirill V. Kovtunov, Dmitry Lebedev, Evgeniy Yu. Gerasimov, Andrey V. Bukhtiyarov, Igor P. Prosvirin, et al. “Spatially Resolved NMR Spectroscopy of Heterogeneous Gas Phase Hydrogenation of 1,3-Butadiene with Para-Hydrogen.” Catalysis Science & Technology 10, no. 1 (2020): 99–104.
https://doi.org/10.1039/C9CY02100K.
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08-17-2020 11:58 PM
Spatially resolved NMR spectroscopy of heterogeneous gas phase hydrogenation of 1,3-butadiene with para-hydrogen
From The DNP-NMR Blog:
Spatially resolved NMR spectroscopy of heterogeneous gas phase hydrogenation of 1,3-butadiene with para-hydrogen
Svyatova, Alexandra, Elizaveta S. Kononenko, Kirill V. Kovtunov, Dmitry Lebedev, Evgeniy Yu. Gerasimov, Andrey V. Bukhtiyarov, Igor P. Prosvirin, et al. “Spatially Resolved NMR Spectroscopy of Heterogeneous Gas Phase Hydrogenation of 1,3-Butadiene with Para Hydrogen.” Catalysis Science & Technology 10, no. 1 (2020): 99–104
https://doi.org/10.1039/C9CY02100K.
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08-07-2020 08:10 PM
Journal Highlight: Mutual diffusion driven NMR: a new approach for the analysis of mixtures by spatially resolved NMR spectroscopy
Journal Highlight: Mutual diffusion driven NMR: a new approach for the analysis of mixtures by spatially resolved NMR spectroscopy
http://www.spectroscopynow.com/common/images/thumbnails/15c4eade5e6.jpgA new approach has been established for resolving the NMR spectra of mixtures, relying on the mutual diffusion of dissolved species when a concentration gradient is established within the NMR tube.
Read the rest at Spectroscopynow.com
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05-29-2017 09:59 AM
Aliphatic chain length by isotropic mixing (ALCHIM): determining composition of complex lipid samples by 1H NMR spectroscopy
Aliphatic chain length by isotropic mixing (ALCHIM): determining composition of complex lipid samples by 1H NMR spectroscopy
Abstract
Quantifying the amounts and types of lipids present in mixtures is important in fields as diverse as medicine, food science, and biochemistry. Nuclear magnetic resonance (NMR) spectroscopy can quantify the total amounts of saturated and unsaturated fatty acids in mixtures, but identifying the length of saturated fatty acid or the position of unsaturation by NMR is a daunting challenge. We have developed an NMR...
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06-19-2014 10:21 PM
[NMR paper] Spatially Selective Heteronuclear Multiple-Quantum Coherence Spectroscopy for Biomolecular NMR Studies.
Spatially Selective Heteronuclear Multiple-Quantum Coherence Spectroscopy for Biomolecular NMR Studies.
Related Articles Spatially Selective Heteronuclear Multiple-Quantum Coherence Spectroscopy for Biomolecular NMR Studies.
Chemphyschem. 2014 Apr 30;
Authors: Sathyamoorthy B, Parish DM, Montelione GT, Xiao R, Szyperski T
Abstract
Spatially selective heteronuclear multiple-quantum coherence (SS HMQC) NMR spectroscopy is developed for solution studies of proteins. Due to "time-staggered" acquisitioning of free induction decays...
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05-03-2014 10:42 PM
Spatially encoded strategies in the execution of biomolecular-oriented 3D NMR experiments
Spatially encoded strategies in the execution of biomolecular-oriented 3D NMR experiments
Mor Mishkovsky, Maayan Gal and Lucio Frydman
Journal of Biomolecular NMR; 2007; 39(4); pp 291-301
Abstract:
Three-dimensional nuclear magnetic resonance (3D NMR) provides one of the foremost analytical tools available for the elucidation of biomolecular structure, function and dynamics. Executing a 3D NMR experiment generally involves scanning a series of time-domain signals S(t 3), as a function of two time variables (t 1, t 2) which need to undergo parametric incrementations throughout...
Determining the Physico-Chemical Composition of Biomolecular Condensates from Spatially-Resolved NMR
Linear Mode
