NMR paper Determining the Diastereoselectivity of the Formation of Dipeptidonucleotides by NMR Spectroscopy

		BioNMR > Educational resources > Journal club
[NMR paper] Determining the Diastereoselectivity of the Formation of Dipeptidonucleotides by NMR Spectroscopy

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

07-23-2021, 01:54 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Determining the Diastereoselectivity of the Formation of Dipeptidonucleotides by NMR Spectroscopy

Determining the Diastereoselectivity of the Formation of Dipeptidonucleotides by NMR Spectroscopy

Proteins are composed of l-amino acids, but nucleic acids and most oligosaccharides contain d-sugars as building blocks. It is interesting to ask whether this is a coincidence or a consequence of the functional interplay of these biomolecules. One reaction that provides an opportunity to study this interplay is the formation of phosphoramidate-linked peptido RNA from amino acids and ribonucleotides in aqueous condensation buffer. Here we report how the diastereoselectivity of the first peptide...

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Multiple-State Monitoring of SOD1 Amyloid Formation at Single-Residue Resolution by Rheo-NMR Spectroscopy Multiple-State Monitoring of SOD1 Amyloid Formation at Single-Residue Resolution by Rheo-NMR Spectroscopy Formation of protein aggregates or fibrils entails the conversion of soluble native protein monomers via multiple molecular states. No spectroscopic techniques have succeeded in capturing the transient molecular-scale events of fibrillation in situ. Here we report residue- and state-specific real-time monitoring of the fibrillation of amyotrophic lateral sclerosis-related SOD1 by rheology NMR (Rheo-NMR) spectroscopy. Under moderately denaturing conditions, where NMR signals of folded...	nmrlearner	Journal club	0	07-08-2021 11:44 AM
[NMR paper] Analysis of ?2AR-Gs and ?2AR-Gi complex formation by NMR spectroscopy. Analysis of ?2AR-Gs and ?2AR-Gi complex formation by NMR spectroscopy. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-custom-pnas_full_free.gif Related Articles Analysis of ?2AR-Gs and ?2AR-Gi complex formation by NMR spectroscopy. Proc Natl Acad Sci U S A. 2020 Aug 31;: Authors: Ma X, Hu Y, Batebi H, Heng J, Xu J, Liu X, Niu X, Li H, Hildebrand PW, Jin C, Kobilka BK Abstract The ?2-adrenergic receptor (?2AR) is a prototypical G protein-coupled receptor...	nmrlearner	Journal club	0	09-13-2020 09:18 AM
[NMR paper] Determining Binding Kinetics of Intrinsically Disordered Proteins by NMR Spectroscopy. Determining Binding Kinetics of Intrinsically Disordered Proteins by NMR Spectroscopy. Related Articles Determining Binding Kinetics of Intrinsically Disordered Proteins by NMR Spectroscopy. Methods Mol Biol. 2020;2141:663-681 Authors: Yang K, Arai M, Wright PE Abstract The unique structural flexibility of intrinsically disordered proteins (IDPs) is central to their diverse functions in cellular processes. Protein-protein interactions involving IDPs are frequently transient and dynamic in nature. Nuclear magnetic resonance...	nmrlearner	Journal club	0	07-23-2020 11:23 PM
[NMR paper] Formation of ?-carrageenan-gelatin polyelectrolyte complexes studied by (1)H NMR, UV spectroscopy and kinematic viscosity measurements. Formation of ?-carrageenan-gelatin polyelectrolyte complexes studied by (1)H NMR, UV spectroscopy and kinematic viscosity measurements. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Formation of ?-carrageenan-gelatin polyelectrolyte complexes studied by (1)H NMR, UV spectroscopy and kinematic viscosity measurements. Carbohydr Polym. 2016 Oct 20;151:1152-61 Authors: Voron'ko NG, Derkach SR, Vovk MA, Tolstoy PM Abstract The intermolecular...	nmrlearner	Journal club	0	01-25-2018 05:54 AM
[NMR paper] Formation of the chaperonin complex studied by 2D NMR spectroscopy. Formation of the chaperonin complex studied by 2D NMR spectroscopy. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--journals.plos.org-plosone-resource-img-external-pone_120x30.png http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Formation of the chaperonin complex studied by 2D NMR spectroscopy. PLoS One. 2017;12(10):e0187022 Authors: Takenaka T, Nakamura T, Yanaka S, Yagi-Utsumi M, Chandak MS, Takahashi K, Paul S, Makabe K, Arai...	nmrlearner	Journal club	0	11-14-2017 04:20 PM
Aliphatic chain length by isotropic mixing (ALCHIM): determining composition of complex lipid samples by 1H NMR spectroscopy Aliphatic chain length by isotropic mixing (ALCHIM): determining composition of complex lipid samples by 1H NMR spectroscopy Abstract Quantifying the amounts and types of lipids present in mixtures is important in fields as diverse as medicine, food science, and biochemistry. Nuclear magnetic resonance (NMR) spectroscopy can quantify the total amounts of saturated and unsaturated fatty acids in mixtures, but identifying the length of saturated fatty acid or the position of unsaturation by NMR is a daunting challenge. We have developed an NMR...	nmrlearner	Journal club	0	06-19-2014 10:21 PM
[NMR paper] CH-? interaction in VQIVYK sequence elucidated by NMR spectroscopy is essential for PHF formation of tau. CH-? interaction in VQIVYK sequence elucidated by NMR spectroscopy is essential for PHF formation of tau. CH-? interaction in VQIVYK sequence elucidated by NMR spectroscopy is essential for PHF formation of tau. Biopolymers. 2014 Mar 29; Authors: Sogawa K, Minoura K, In Y, Ishida T, Taniguchi T, Tomoo K Abstract One of the histopathological features of Alzheimer's disease (AD) is higher order neurofibrillary tangles formed by abnormally aggregated tau protein. Investigation of the mechanism of tau aggregation is important for...	nmrlearner	Journal club	0	04-02-2014 11:54 PM
Role of Hydrophobic Interactions in the EncounterComplex Formation of the Plastocyanin and Cytochrome f Complex Revealed by Paramagnetic NMR Spectroscopy Role of Hydrophobic Interactions in the EncounterComplex Formation of the Plastocyanin and Cytochrome f Complex Revealed by Paramagnetic NMR Spectroscopy Sandra Scanu, Johannes M. Foerster, G. Matthias Ullmann and Marcellus Ubbink http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja4015452/aop/images/medium/ja-2013-015452_0009.gif Journal of the American Chemical Society DOI: 10.1021/ja4015452 http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/I9ARcKlvRs8	nmrlearner	Journal club	0	05-15-2013 02:51 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off