Publication date: Available online 11 July 2016 Source:Analytical Biochemistry
Author(s): Cátia Marques, Filipa Viegas, João Rito, John Jones, Ivan Viegas
Following administration of deuterated water (2H2O), the fractional synthetic rate (FSR) of a given endogenous protein can be estimated by 2H-enrichment quantification of its alanine residues. Currently, this is measured by mass spectrometry following a derivatization procedure. Muscle FSR was measured by 1H/2H NMR analysis of alanine from seabass kept for 6 days in 5% 2H-enriched saltwater, following acid hydrolysis and amino acid isolation by cation-exchange chromatography of muscle tissue. The analysis is simple and robust, and provides precise measurements of excess alanine 2H-enrichment in the 0.1–0.4% range from 50*mmol of alanine recovered from muscle protein.
Accurate determination of rates from non-uniformly sampled relaxation data
Accurate determination of rates from non-uniformly sampled relaxation data
Abstract
The application of non-uniform sampling (NUS) to relaxation experiments traditionally used to characterize the fast internal motion of proteins is quantitatively examined. Experimentally acquired Poisson-gap sampled data reconstructed with iterative soft thresholding are compared to regular sequentially sampled (RSS) data. Using ubiquitin as a model system, it is shown that 25Â*% sampling is sufficient for the determination of quantitatively accurate relaxation...
[NMR paper] Toward a Biorelevant Structure of Protein Kinase C Bound Modulators: Design, Synthesis, and Evaluation of Labeled Bryostatin Analogs for Analysis with REDOR NMR Spectroscopy.
Toward a Biorelevant Structure of Protein Kinase C Bound Modulators: Design, Synthesis, and Evaluation of Labeled Bryostatin Analogs for Analysis with REDOR NMR Spectroscopy.
Toward a Biorelevant Structure of Protein Kinase C Bound Modulators: Design, Synthesis, and Evaluation of Labeled Bryostatin Analogs for Analysis with REDOR NMR Spectroscopy.
J Am Chem Soc. 2015 Feb 24;
Authors: Loy BA, Lesser AB, Staveness D, Billingsley KL, Cegelski L, Wender PA
Abstract
Protein kinase C (PKC) modulators are currently of great...
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[NMR paper] Determination of accurate 1H positions of an alanine tripeptide with anti-parallel and parallel ?-sheet structures by high resolution 1H solid state NMR and GIPAW chemical shift calculation.
Determination of accurate 1H positions of an alanine tripeptide with anti-parallel and parallel ?-sheet structures by high resolution 1H solid state NMR and GIPAW chemical shift calculation.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles Determination of accurate 1H positions of an alanine tripeptide with anti-parallel and parallel ?-sheet structures by high resolution 1H solid state NMR and GIPAW chemical shift calculation.
Chem Commun (Camb). 2012 Nov 25;48(91):11199-201
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[Question from NMRWiki Q&A forum] How to catch Deviate Fish wow4.2
How to catch Deviate Fish wow4.2
4.2 map is changed, my fishing to 150, but in the Barrens that what fishing oasis in nearly an hour, a variation of the fish did not catch wow gold ah, who knows where to catch ah?
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[NMR paper] Determination of the electron relaxation rates in paramagnetic metal complexes: appli
Determination of the electron relaxation rates in paramagnetic metal complexes: applicability of available NMR methods.
Related Articles Determination of the electron relaxation rates in paramagnetic metal complexes: applicability of available NMR methods.
J Magn Reson. 2004 Apr;167(2):169-77
Authors: Jensen MR, Led JJ
Four different approaches for determining the electron relaxation rates in paramagnetic metallo-proteins are investigated, using a paramagnetic Ni2+ complex of a protein as an example. All four approaches rely on the...
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[NMR paper] Yeast expression and NMR analysis of the extracellular domain of muscle nicotinic ace
Yeast expression and NMR analysis of the extracellular domain of muscle nicotinic acetylcholine receptor alpha subunit.
Related Articles Yeast expression and NMR analysis of the extracellular domain of muscle nicotinic acetylcholine receptor alpha subunit.
J Biol Chem. 2002 Apr 12;277(15):12613-21
Authors: Yao Y, Wang J, Viroonchatapan N, Samson A, Chill J, Rothe E, Anglister J, Wang ZZ
The alpha subunit of the nicotinic acetylcholine receptor (AChR) from Torpedo electric organ and mammalian muscle contains high affinity binding sites for...
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Structure determination of proteins in 2H2O solution aided by a deuterium-decoupled 3
Abstract We developed an NMR pulse sequence, 3D HCA(N)CO, to correlate the chemical shifts of protein backbone 1Hα and 13Cα to those of 13C� in the preceding residue. By applying 2H decoupling, the experiment was accomplished with high sensitivity comparable to that of HCA(CO)N. When combined with HCACO, HCAN and HCA(CO)N, the HCA(N)CO sequence allows the sequential assignment using backbone 13C� and amide 15N chemical shifts without resort to backbone amide protons. This assignment strategy was demonstrated for 13C/15N-labeled GB1 dissolved in 2H2O. The quality of the GB1 structure...
Determination of muscle protein synthesis rates in fish using 2H2O and 2H NMR analysis of alanine
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