Related ArticlesDetermination of muscle protein synthesis rates in fish using (2)H2O and (2)H NMR analysis of alanine.
Anal Biochem. 2016 Jul 11;
Authors: Marques C, Viegas F, Rito J, Jones J, Viegas I
Abstract
Following administration of deuterated water ((2)H2O), the fractional synthetic rate (FSR) of a given endogenous protein can be estimated by (2)H-enrichment quantification of its alanine residues. Currently, this is measured by mass spectrometry following a derivatization procedure. Muscle FSR was measured by (1)H/(2)H NMR analysis of alanine from seabass kept for 6 days in 5% (2)H-enriched saltwater, following acid hydrolysis and amino acid isolation by cation-exchange chromatography of muscle tissue. The analysis is simple and robust, and provides precise measurements of excess alanine (2)H-enrichment in the 0.1-0.4% range from 50*mmol of alanine recovered from muscle protein.
PMID: 27418547 [PubMed - as supplied by publisher]
Synthesis and Kinetic Analysis of Two ConformationallyRestricted Peptide Substrates of Escherichia coli Penicillin-Binding Protein 5
Synthesis and Kinetic Analysis of Two ConformationallyRestricted Peptide Substrates of Escherichia coli Penicillin-Binding Protein 5
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b00576/20160715/images/medium/bi-2016-00576v_0011.gif
Biochemistry
DOI: 10.1021/acs.biochem.6b00576
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[NMR paper] Alanine scan and (2)H NMR analysis of the membrane-active peptide BP100 point to a distinct carpet mechanism of action.
Alanine scan and (2)H NMR analysis of the membrane-active peptide BP100 point to a distinct carpet mechanism of action.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Alanine scan and (2)H NMR analysis of the membrane-active peptide BP100 point to a distinct carpet mechanism of action.
Biochim Biophys Acta. 2016 Jun;1858(6):1328-38
Authors: Zamora-Carreras H, Strandberg E, Mühlhäuser P, Bürck J, Wadhwani P, Jiménez MÁ, Bruix M, Ulrich AS
Abstract...
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Determination of muscle protein synthesis rates in fish using 2H2O and 2H NMR analysis of alanine
Determination of muscle protein synthesis rates in fish using 2H2O and 2H NMR analysis of alanine
Publication date: Available online 11 July 2016
Source:Analytical Biochemistry</br>
Author(s): Cátia Marques, Filipa Viegas, João Rito, John Jones, Ivan Viegas</br>
Following administration of deuterated water (2H2O), the fractional synthetic rate (FSR) of a given endogenous protein can be estimated by 2H-enrichment quantification of its alanine residues. Currently, this is measured by mass spectrometry following a derivatization procedure. Muscle FSR was...
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07-11-2016 04:06 PM
Accurate determination of rates from non-uniformly sampled relaxation data
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Abstract
The application of non-uniform sampling (NUS) to relaxation experiments traditionally used to characterize the fast internal motion of proteins is quantitatively examined. Experimentally acquired Poisson-gap sampled data reconstructed with iterative soft thresholding are compared to regular sequentially sampled (RSS) data. Using ubiquitin as a model system, it is shown that 25Â*% sampling is sufficient for the determination of quantitatively accurate relaxation...
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Authors: Yao Y, Wang J, Viroonchatapan N, Samson A, Chill J, Rothe E, Anglister J, Wang ZZ
The alpha subunit of the nicotinic acetylcholine receptor (AChR) from Torpedo electric organ and mammalian muscle contains high affinity binding sites for...
Determination of muscle protein synthesis rates in fish using (2)H2O and (2)H NMR analysis of alanine.
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