NMR paper Determination of muscle protein synthesis rates in fish using (2)H2O and (2)H NMR analysis of alanine.

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[NMR paper] Determination of muscle protein synthesis rates in fish using (2)H2O and (2)H NMR analysis of alanine.

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07-16-2016, 10:22 PM

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Determination of muscle protein synthesis rates in fish using (2)H2O and (2)H NMR analysis of alanine.

Determination of muscle protein synthesis rates in fish using (2)H2O and (2)H NMR analysis of alanine.

Related Articles Determination of muscle protein synthesis rates in fish using (2)H2O and (2)H NMR analysis of alanine.

Anal Biochem. 2016 Jul 11;

Authors: Marques C, Viegas F, Rito J, Jones J, Viegas I

Abstract
Following administration of deuterated water ((2)H2O), the fractional synthetic rate (FSR) of a given endogenous protein can be estimated by (2)H-enrichment quantification of its alanine residues. Currently, this is measured by mass spectrometry following a derivatization procedure. Muscle FSR was measured by (1)H/(2)H NMR analysis of alanine from seabass kept for 6 days in 5% (2)H-enriched saltwater, following acid hydrolysis and amino acid isolation by cation-exchange chromatography of muscle tissue. The analysis is simple and robust, and provides precise measurements of excess alanine (2)H-enrichment in the 0.1-0.4% range from 50*mmol of alanine recovered from muscle protein.

PMID: 27418547 [PubMed - as supplied by publisher]

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