NMR paper Determination of the diffusion coefficient of insulin and lysozyme in crosslinked dex

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[NMR paper] Determination of the diffusion coefficient of insulin and lysozyme in crosslinked dex

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NMR processing:

MDD

NMR assignment:

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Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

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GeNMR

I-TASSER

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Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

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TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

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RCI

Interactions from chemical shifts:

HADDOCK

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NOEs, other restraints:

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SAVES2 or SAVES4

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Proshift

PPM

CheShift-2- Cα

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Shifty

Camcoil

Poulsen_rc_CS

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NMR sample preparation:

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11-17-2010, 11:15 PM

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Determination of the diffusion coefficient of insulin and lysozyme in crosslinked dex

Determination of the diffusion coefficient of insulin and lysozyme in crosslinked dextran hydrogels by pulsed-field-gradient NMR.

Related Articles Determination of the diffusion coefficient of insulin and lysozyme in crosslinked dextran hydrogels by pulsed-field-gradient NMR.

Chem Pharm Bull (Tokyo). 1998 Nov;46(11):1836-9

Authors: Aso Y, Yoshioka S, Kojima S

Crosslinked dextran hydrogels were prepared from glycidyl methacrylate (GMA)-derivatized dextran by gamma-irradiation initiation in the presence of insulin and lysozyme as model protein and peptide drugs. The diffusion coefficient of insulin and lysozyme in the hydrogels was determined successfully by a pulsed-field-gradient stimulated-echo method. A conventional NMR probe which can impose a relatively small magnetic field gradient (30 Gauss/cm) was used for the measurements. This method seems to be applicable to proteins and peptides with a molecular weight of about 10,000 or less. The diffusion coefficient determined by the NMR method almost agreed with that calculated from the drug release profiles, indicating the usefulness of the diffusion coefficient determined by the NMR method as a measure of the release rate from hydrogels.

PMID: 9845963 [PubMed - indexed for MEDLINE]

Source: PubMed

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