[NMR paper] Determination of the binding epitope of RGD-peptidomimetics to ?v?3 and ?(IIb)?3 integrin-rich intact cells by NMR and computational studies.
Related ArticlesDetermination of the binding epitope of RGD-peptidomimetics to ?v?3 and ?(IIb)?3 integrin-rich intact cells by NMR and computational studies.
Org Biomol Chem. 2013 Jun 21;11(23):3886-93
Authors: Guzzetti I, Civera M, Vasile F, Araldi EM, Belvisi L, Gennari C, Potenza D, Fanelli R, Piarulli U
Abstract
NMR experiments (transferred NOE and Saturation Transfer Difference) were used to shed light on the binding epitope of RGD peptidomimetics 1-3 with integrins ?v?3 and ?(IIb)?3, expressed on the membrane of ECV304 bladder cancer cells and human platelets, respectively. The NMR results were supported by docking calculations of 1-3 in the active sites of ?v?3 and ?(IIb)?3 integrin receptors and were compared to the results of competitive ?v?3 receptor binding assays and competitive ECV304 cell adhesion experiments. While cis RGD ligand 1 interacts mainly with the ? integrin subunit through its basic guanidine group, trans RGD ligands 2 and 3 are able to interact with both the ? and ? integrin subunits via an electrostatic clamp.
[NMR paper] Direct determination of the interleukin-6 binding epitope of the interleukin-6 recept
Direct determination of the interleukin-6 binding epitope of the interleukin-6 receptor by NMR spectroscopy.
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J Biol Chem. 2004 Jan 2;279(1):571-6
Authors: Schwantner A, Dingley AJ, Ozbek S, Rose-John S, Grötzinger J
All cytokines belonging to the interleukin-6 (IL-6)-type family of cytokines utilize receptors that have a modular build of several immunoglobulin-like and fibronectin type III-like domains. Characteristic...
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[NMR paper] NMR-based determination of the binding epitope and conformational analysis of MUC-1 g
NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3.
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Eur J Biochem. 2002 Mar;269(5):1444-55
Authors: Möller H, Serttas N, Paulsen H, Burchell JM, Taylor-Papadimitriou J,
Mucin glycoproteins on breast cancer cells carry shortened...
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[NMR paper] NMR spectroscopy and chemical studies of an arabinan-rich system from the endosperm o
NMR spectroscopy and chemical studies of an arabinan-rich system from the endosperm of the seed of Gleditsia triacanthos.
Related Articles NMR spectroscopy and chemical studies of an arabinan-rich system from the endosperm of the seed of Gleditsia triacanthos.
Carbohydr Res. 2002 Feb 11;337(3):255-63
Authors: Navarro DA, Cerezo AS, Stortz CA
Exhaustive extraction of the endosperm from the seed of Gleditsia triacanthos using water at room temperature and 50 degrees C left a residue, which was further extracted at 95 degrees C. Precipitation of...
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[NMR paper] Structures of the platelet calcium- and integrin-binding protein and the alphaIIb-int
Structures of the platelet calcium- and integrin-binding protein and the alphaIIb-integrin cytoplasmic domain suggest a mechanism for calcium-regulated recognition; homology modelling and NMR studies.
Related Articles Structures of the platelet calcium- and integrin-binding protein and the alphaIIb-integrin cytoplasmic domain suggest a mechanism for calcium-regulated recognition; homology modelling and NMR studies.
J Mol Recognit. 2000 Mar-Apr;13(2):83-92
Authors: Hwang PM, Vogel HJ
Calcium- and integrin-binding protein (CIB) binds to the...
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11-18-2010 09:15 PM
[NMR paper] The insulin-like growth factor (IGF)binding protein 1 binding epitope on IGF-I probed
The insulin-like growth factor (IGF)binding protein 1 binding epitope on IGF-I probed by heteronuclear NMR spectroscopy and mutational analysis.
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J Biol Chem. 1998 Sep 18;273(38):24701-7
Authors: Jansson M, Andersson G, Uhlén M, Nilsson B, Kördel J
NMR spectroscopy studies and biosensor interaction analysis of native and site-directed mutants of insulin-like growth factor I (IGF-I) was...
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[NMR paper] Structure of the metal-free gamma-carboxyglutamic acid-rich membrane binding region o
Structure of the metal-free gamma-carboxyglutamic acid-rich membrane binding region of factor IX by two-dimensional NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-standard-jbc_full_free.gif Related Articles Structure of the metal-free gamma-carboxyglutamic acid-rich membrane binding region of factor IX by two-dimensional NMR spectroscopy.
J Biol Chem. 1995 Apr 7;270(14):7980-7
Authors: Freedman SJ, Furie BC, Furie B, Baleja JD
The gamma-carboxyglutamic acid-rich...
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[NMR paper] 1H-NMR investigation of the oxygenation of hemoglobin in intact human red blood cells
1H-NMR investigation of the oxygenation of hemoglobin in intact human red blood cells.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-cellhub.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles 1H-NMR investigation of the oxygenation of hemoglobin in intact human red blood cells.
Biophys J. 1995 Feb;68(2):681-93
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Using improved selective excitation methods for protein nuclear...
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[NMR paper] High-resolution magic angle spinning 1H NMR spectroscopic studies on intact rat renal
High-resolution magic angle spinning 1H NMR spectroscopic studies on intact rat renal cortex and medulla.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_120x27.gif Related Articles High-resolution magic angle spinning 1H NMR spectroscopic studies on intact rat renal cortex and medulla.
Magn Reson Med. 1999 Jun;41(6):1108-18
Authors: Garrod S, Humpfer E, Spraul M, Connor SC, Polley S, Connelly J, Lindon JC, Nicholson JK, Holmes E
High-resolution magic angle...
Determination of the binding epitope of RGD-peptidomimetics to ?v?3 and ?(IIb)?3 integrin-rich intact cells by NMR and computational studies.
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