Pin1 is a peptidyl-prolyl cis-trans isomerase with specificity towards the pSer/pThr-Pro protein motifs. Through its cis-trans isomerization activity, Pin1 regulates the stability of oncogenic and tumor suppressor proteins. The involvement of Pin1 in cancer development makes it an attractive target for therapeutic intervention. To that end, a wide variety of small-molecule inhibitors were developed to specifically target Pin1. A major challenge that remains in the Pin1 field is the high...
[NMR paper] Ligand-Based NMR Study of C-X-C Chemokine Receptor Type 4 (CXCR4)-Ligand Interactions on Living Cancer Cells.
Ligand-Based NMR Study of C-X-C Chemokine Receptor Type 4 (CXCR4)-Ligand Interactions on Living Cancer Cells.
Ligand-Based NMR Study of C-X-C Chemokine Receptor Type 4 (CXCR4)-Ligand Interactions on Living Cancer Cells.
J Med Chem. 2018 Mar 09;:
Authors: Brancaccio D, Diana D, Di Maro S, Di Leva FS, Tomassi S, Fattorusso R, Russo L, Scala S, Trotta AM, Portella L, Novellino E, Marinelli L, Carotenuto A
Abstract
Peptides-binding G protein-coupled receptors play an important role in many pathological and physiological pathways....
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03-10-2018 04:36 PM
[NMR paper] Identification of Ligand-Receptor Interactions: Ligand Molecular Arrays, SPR and NMR Methodologies.
Identification of Ligand-Receptor Interactions: Ligand Molecular Arrays, SPR and NMR Methodologies.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Identification of Ligand-Receptor Interactions: Ligand Molecular Arrays, SPR and NMR Methodologies.
Methods Mol Biol. 2017;1512:51-63
Authors: Day CJ, Hartley-Tassell LE, Korolik V
Abstract
Despite many years of research into bacterial chemotaxis, the only well characterized system...
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01-11-2018 04:07 PM
[NMR paper] Investigating protein-ligand interactions by solution NMR spectroscopy.
Investigating protein-ligand interactions by solution NMR spectroscopy.
Investigating protein-ligand interactions by solution NMR spectroscopy.
Chemphyschem. 2018 Jan 04;:
Authors: Becker W, Bhattiprolu KC, Gubensäk N, Zangger K
Abstract
Protein-ligand interactions are of fundamental importance in almost all processes in living organisms. The ligands comprise small molecules, drugs or biological macromolecules and their interaction strength varies over several orders of magnitude. Solution NMR spectroscopy offers a large...
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01-10-2018 12:45 PM
[NMR paper] Target-specific NMR detection of protein-ligand interactions with antibody-relayed (15)N-group selective STD.
Target-specific NMR detection of protein-ligand interactions with antibody-relayed (15)N-group selective STD.
Target-specific NMR detection of protein-ligand interactions with antibody-relayed (15)N-group selective STD.
J Biomol NMR. 2016 Nov 24;
Authors: Hetényi A, Heged?s Z, Fajka-Boja R, Monostori É, Kövér KE, Martinek TA
Abstract
Fragment-based drug design has been successfully applied to challenging targets where the detection of the weak protein-ligand interactions is a key element. (1)H saturation transfer difference...
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11-26-2016 12:27 PM
Target-specific NMR detection of proteinâ??ligand interactions with antibody-relayed 15 N-group selective STD
Target-specific NMR detection of proteinâ??ligand interactions with antibody-relayed 15 N-group selective STD
Abstract
Fragment-based drug design has been successfully applied to challenging targets where the detection of the weak proteinâ??ligand interactions is a key element. 1H saturation transfer difference (STD) NMR spectroscopy is a powerful technique for this work but it requires pure homogeneous proteins as targets. Monoclonal antibody (mAb)-relayed 15N-GS STD spectroscopy has been developed to resolve the problem of protein mixtures and...
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11-25-2016 02:22 AM
[NMR paper] Analyzing protein-ligand interactions by dynamic NMR spectroscopy.
Analyzing protein-ligand interactions by dynamic NMR spectroscopy.
Related Articles Analyzing protein-ligand interactions by dynamic NMR spectroscopy.
Methods Mol Biol. 2013;1008:243-66
Authors: Mittermaier A, Meneses E
Abstract
Nuclear magnetic resonance (NMR) spectroscopy can provide detailed information on protein-ligand interactions that is inaccessible using other biophysical techniques. This chapter focuses on NMR-based approaches for extracting affinity and rate constants for weakly binding transient protein complexes with lifetimes...
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06-05-2013 06:53 PM
Clean STD-NMR spectrum for improved detection of ligand-protein interactions at low c
Clean STD-NMR spectrum for improved detection of ligand-protein interactions at low concentration of protein.
Related Articles Clean STD-NMR spectrum for improved detection of ligand-protein interactions at low concentration of protein.
Magn Reson Chem. 2010 Oct 18;
Authors: Xia Y, Zhu Q, Jun KY, Wang J, Gao X
Saturation transfer difference (STD)-NMR has been widely used to screen ligand compound libraries for their binding activities to proteins and to determine the binding epitopes of the ligands. We report herein, a Clean STD-NMR method...
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10-22-2010 06:02 AM
[NMR paper] On the ligand-protein and ligand-flavin interactions in NADPH-adrenodoxin reductase a
On the ligand-protein and ligand-flavin interactions in NADPH-adrenodoxin reductase as studied by 31P- and 13C-NMR. Use of 13C-enriched FAD as a probe.
Related Articles On the ligand-protein and ligand-flavin interactions in NADPH-adrenodoxin reductase as studied by 31P- and 13C-NMR. Use of 13C-enriched FAD as a probe.
J Biochem. 1991 Jan;109(1):144-9
Authors: Fujii S, Nonaka Y, Okamoto M, Miura R
The interaction between 2',5'-ADP and NADPH-adrenodoxin reductase from bovine adrenocortical mitochondria was examined by titrating the enzyme with...
Detection of the Pin1-ligand interactions using 19 F NMR spectroscopy
Linear Mode
