NMR paper Detecting Bound Ions in Ion Channels by Solid-State NMR Experiments on (15)N-Labelled Ammonium Ions

		BioNMR > Educational resources > Journal club
[NMR paper] Detecting Bound Ions in Ion Channels by Solid-State NMR Experiments on (15)N-Labelled Ammonium Ions

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

06-10-2024, 11:03 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,778

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Detecting Bound Ions in Ion Channels by Solid-State NMR Experiments on (15)N-Labelled Ammonium Ions

Detecting Bound Ions in Ion Channels by Solid-State NMR Experiments on (15)N-Labelled Ammonium Ions

Solid-state NMR allows for the study of membrane proteins under physiological conditions. Here we describe a method for detection of bound ions in the selectivity filter of ion channels using solid-state NMR. This method employs standard ¹H-detected solid-state NMR setup and experiment types, which is enabled by using ^(15)N-labelled ammonium ions to mimic potassium ions.

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Raman, WAXS, and Solid-State NMR Characterizations of Regenerated Silk Fibroin Using Lanthanide Ions as Chaotropic Agents Raman, WAXS, and Solid-State NMR Characterizations of Regenerated Silk Fibroin Using Lanthanide Ions as Chaotropic Agents Bombyx mori silk fibroin (SF) has been reported as a convenient natural material for regenerative medicine, optoelectronics, and many other technological applications. SF owes its unique features to the hierarchical organization of the fibers. Many efforts have been made to set up protocols for dissolution since many applications of SF are based on regenerated solutions and fibers, but chaotropic conditions required to disassemble the packing of the polymer afford...	nmrlearner	Journal club	0	07-17-2023 01:09 PM
[ASAP] Direct Detection of Bound Ammonium Ions in the Selectivity Filter of Ion Channels by Solid-State NMR Direct Detection of Bound Ammonium Ions in the Selectivity Filter of Ion Channels by Solid-State NMR Carl O?ster, Kumar Tekwani Movellan, Benjamin Goold, Kitty Hendriks, Sascha Lange, Stefan Becker, Bert L. de Groot, Wojciech Kopec, Loren B. Andreas, and Adam Lange https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.1c13247/20220224/images/medium/ja1c13247_0008.gif Journal of the American Chemical Society DOI: 10.1021/jacs.1c13247	nmrlearner	Journal club	0	02-25-2022 07:54 PM
Enabling Natural Abundance 17O Solid-State NMR by Direct Polarization from Paramagnetic Metal Ions #DNPNMR From The DNP-NMR Blog: Enabling Natural Abundance 17O Solid-State NMR by Direct Polarization from Paramagnetic Metal Ions #DNPNMR Jardón-Álvarez, Daniel, Guy Reuveni, Adi Harchol, and Michal Leskes. “Enabling Natural Abundance 17O Solid-State NMR by Direct Polarization from Paramagnetic Metal Ions.” The Journal of Physical Chemistry Letters 11, no. 14 (July 16, 2020): 5439–45. https://doi.org/10.1021/acs.jpclett.0c01527.	nmrlearner	News from NMR blogs	0	10-19-2020 05:07 PM
[NMR paper] Zooming into the Interaction of ?-synuclein with Calcium Ions through Exclusively Heteronuclear NMR Experiments. Zooming into the Interaction of ?-synuclein with Calcium Ions through Exclusively Heteronuclear NMR Experiments. Related Articles Zooming into the Interaction of ?-synuclein with Calcium Ions through Exclusively Heteronuclear NMR Experiments. Angew Chem Int Ed Engl. 2020 Jul 31;: Authors: Pontoriero L, Schiavina M, Murrali MG, Pierattelli R, Felli IC Abstract Many properties of intrinsically disordered proteins (IDPs) or protein regions (IDRs) are expected to be modulated by the nature of amino acid side chains as well as by...	nmrlearner	Journal club	0	08-01-2020 02:01 PM
Dynamic channels: NMR tracks the ions - spectroscopyNOW.com http://www.bionmr.com//t0.gstatic.com/images?q=tbn:ANd9GcRlCAGEohsKAVcoshTCJ76xNHgrdiyfoF3pmPA1-2je3pasjoHgdW_AXI9_YkxcYiXKy69ezHX2 spectroscopyNOW.com <img alt="" height="1" width="1"> Dynamic channels: NMR tracks the ions spectroscopyNOW.com Researchers in Germany have demonstrated using nuclear magnetic resonance (NMR) spectroscopy how the selectivity filter of the cell membrane's NaK protein ion channel forms only a single structure to control the flow of potassium ions. The proteins ... Dynamic channels: NMR tracks the ions - spectroscopyNOW.com	nmrlearner	Online News	0	03-01-2018 06:06 AM
Dynamic channels: NMR tracks the ions Dynamic channels: NMR tracks the ions http://www.spectroscopynow.com/common/images/thumbnails/161d1728470.jpgResearchers in Germany have demonstrated using nuclear magnetic resonance (NMR) spectroscopy how the selectivity filter of the cell membrane's NaK protein ion channel forms only a single structure to control the flow of potassium ions. Read the rest at Spectroscopynow.com	nmrlearner	General	0	03-01-2018 06:06 AM
Primostrato Solid-State NMR Enhanced by Dynamic Nuclear Polarization: Pentacoordinated Al3+ Ions Are Only Located at the Surface of Hydrated ?-Alumina From The DNP-NMR Blog: Primostrato Solid-State NMR Enhanced by Dynamic Nuclear Polarization: Pentacoordinated Al3+ Ions Are Only Located at the Surface of Hydrated ?-Alumina Lee, D., et al., Primostrato Solid-State NMR Enhanced by Dynamic Nuclear Polarization: Pentacoordinated Al3+ Ions Are Only Located at the Surface of Hydrated ?-Alumina. The Journal of Physical Chemistry C, 2014. http://dx.doi.org/10.1021/jp508009x	nmrlearner	News from NMR blogs	0	10-27-2014 02:10 PM
Detecting Substrates Bound to the Secondary Multidrug Efflux Pump EmrE by DNP-Enhanced Solid-State NMR Detecting Substrates Bound to the Secondary Multidrug Efflux Pump EmrE by DNP-Enhanced Solid-State NMR Yean Sin Ong, Andrea Lakatos, Johanna Becker-Baldus, Klaas M. Pos and Clemens Glaubitz http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja402605s/aop/images/medium/ja-2013-02605s_0006.gif Journal of the American Chemical Society DOI: 10.1021/ja402605s http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/AjOxCwRFsQs	nmrlearner	Journal club	0	10-12-2013 12:59 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off