Related ArticlesDesign of small volume HX and triple-resonance probes for improved limits of detection in protein NMR experiments.
J Magn Reson. 2003 Sep;164(1):128-35
Authors: Li Y, Logan TM, Edison AS, Webb A
Three- and four-frequency nuclear magnetic-resonance probes have been designed for the study of small amounts of protein. Both "HX" (1H, X, and 2H channels) and "triple-resonance" (1H, 15N, 13C, and 2H) probes were implemented using a single transmit/receive coil and multiple-frequency impedance matching circuits. The coil used was a six-turn solenoid with an observe volume of 15 microl. A variable pitch design was used to improve the B1 homogeneity of the coil. Two-dimensional HSQC spectra of approximately 1mM single labeled 15N- and double labeled 15N/13C-proteins were acquired in experimental times of approximately 2h. Triple-resonance capability of the small-volume triple-resonance probe was demonstrated by acquiring three-dimensional HNCO spectra from the same protein samples. In addition to enabling very small quantities of protein to be used, the extremely short pulse widths (1H = 4, 15N = 4, and 13C = 2 micros) of this particular design result in low power decoupling and wide-bandwidth coverage, an important factor for the ever-higher operating frequencies used for protein NMR studies.
Triple resonance three-dimensional protein NMR: Before it became a black box.
Triple resonance three-dimensional protein NMR: Before it became a black box.
Triple resonance three-dimensional protein NMR: Before it became a black box.
J Magn Reson. 2011 Aug 30;
Authors: Bax A
Abstract
Three-dimensional triple resonance experiments have become an integral part of virtually every solution NMR study of proteins. The approach relies on uniform isotopic enrichment of proteins with (13)C and (15)N, and establishes the scalar connectivity pathway between nuclei through the large (1)J(NH), (1)J(CH)(, 1)J(CC), and (1)J(CN)...
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09-03-2011 06:55 PM
Triple resonance three-dimensional protein NMR: Before it became a black box
Triple resonance three-dimensional protein NMR: Before it became a black box
Publication year: 2011
Source: Journal of Magnetic Resonance, In Press, Corrected Proof, Available online 31 August 2011</br>
Ad, Bax</br>
Three-dimensional triple resonance experiments have become an integral part of virtually every solution NMR study of proteins. The approach relies on uniform isotopic enrichment of proteins with 13C and 15N, and establishes the scalar connectivity pathway between nuclei through the large 1JNH, 1JCH, 1JCC, and 1JCN couplings. The magnetization transfer process takes place...
nmrlearner
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08-31-2011 07:12 PM
Systematic Study of Protein Detection Mechanism of Self-Assembling 19F NMR/MRI Nanoprobes toward Rational Design and Improved Sensitivity
Systematic Study of Protein Detection Mechanism of Self-Assembling 19F NMR/MRI Nanoprobes toward Rational Design and Improved Sensitivity
Yousuke Takaoka, Keishi Kiminami, Keigo Mizusawa, Kazuya Matsuo, Michiko Narazaki, Tetsuya Matsuda and Itaru Hamachi
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja203996c/aop/images/medium/ja-2011-03996c_0004.gif
Journal of the American Chemical Society
DOI: 10.1021/ja203996c
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/fqTSjFalrGg
[NMR paper] 1H,15N,13C-triple resonance NMR of very large systems at 900 MHz.
1H,15N,13C-triple resonance NMR of very large systems at 900 MHz.
Related Articles 1H,15N,13C-triple resonance NMR of very large systems at 900 MHz.
J Magn Reson. 2003 Aug;163(2):360-8
Authors: Chung J, Kroon G
We provide quantitative signal to noise data and feasibility study at 900 MHz for 1H-15N-13C triple resonance backbone assignment pulse sequences obtained from a medium sized 2H, 13C, 15N labeled protein slowed down in glycerol-water solution to mimic relaxation and spectroscopic properties of a much larger protein system with...
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11-24-2010 09:16 PM
[NMR paper] Small volume flow probe for automated direct-injection NMR analysis: design and perfo
Small volume flow probe for automated direct-injection NMR analysis: design and performance.
Related Articles Small volume flow probe for automated direct-injection NMR analysis: design and performance.
J Magn Reson. 2000 Mar;143(1):69-78
Authors: Haner RL, Llanos W, Mueller L
A detailed characterization of an NMR flow probe for use in direct-injection sample analysis is presented. A 600-MHz, indirect detection NMR flow probe with a 120-microl active volume is evaluated in two configurations: first as a stand-alone small volume probe for the...
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11-18-2010 09:15 PM
[Question from NMRWiki Q&A forum] Please suggest experiments that push the power limits of NMR probes
Please suggest experiments that push the power limits of NMR probes
I'm trying to come up with reasonable tests of probe performance for all channels by simulating conditions at which arcing is most likely to occur.
For those tests I usually read voltage of the incoming wave on the RF line using directional coupler with a 50dB attenuated pickup and oscilloscope after running an "experiment" pulsing at the power and duty cycle that simulates the condition to be tested. If the waveform looks nice and square - there is no arcing.
What experiments should I look into? OK for 1H I'd pick...
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10-08-2010 02:35 AM
HNCB Triple Resonance Experiment (Varian)
Hello,
Has anyone come across this experiment? I see it listed but have not been able to find the pulse sequence. I have noticed that in some cases that the reference simply implies the HNCACB experiment running in such a way to allow complete transfer to the CB.
So is there an HNCB or is the only HNCB in the form of the HNCACB with the delay set to allow complete transfer to the CB?
Thanks in advance!!!
Design of small volume HX and triple-resonance probes for improved limits of detectio
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