The design of imaging agents with high fluorine content is essential for overcoming the challenges associated with signal detection limits in ^(19)F MRI-based molecular imaging. In addition to perfluorocarbon and fluorinated polymers, fluorinated peptides offer an additional strategy for creating sequence-defined ^(19)F magnetic resonance imaging (MRI) imaging agents with a high fluorine signal. Our previously reported unstructured trifluoroacetyllysine-based peptides possessed good...
[ASAP] Direct Expression of Fluorinated Proteins in Human Cells for 19F In-Cell NMR Spectroscopy
Direct Expression of Fluorinated Proteins in Human Cells for 19F In-Cell NMR Spectroscopy
Lan B. T. Pham, Azzurra Costantino, Letizia Barbieri, Vito Calderone, Enrico Luchinat, and Lucia Banci
https://pubs.acs.org/cms/10.1021/jacs.2c12086/asset/images/medium/ja2c12086_0007.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.2c12086
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[NMR paper] Direct Expression of Fluorinated Proteins in Human Cells for 19F In-Cell NMR Spectroscopy
Direct Expression of Fluorinated Proteins in Human Cells for 19F In-Cell NMR Spectroscopy
In-cell NMR spectroscopy is a powerful approach to study protein structure and function in the native cellular environment. It provides precious insights into the folding, maturation, interactions, and ligand binding of important pharmacological targets directly in human cells. However, its widespread application is hampered by the fact that soluble globular proteins often interact with large cellular components, causing severe line broadening in conventional heteronuclear NMR experiments....
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[NMR paper] Fluorine-19 NMR spectroscopy of fluorinated analogs of tritrpticin highlights a distinct role for Tyr residues in antimicrobial peptides.
Fluorine-19 NMR spectroscopy of fluorinated analogs of tritrpticin highlights a distinct role for Tyr residues in antimicrobial peptides.
Related Articles Fluorine-19 NMR spectroscopy of fluorinated analogs of tritrpticin highlights a distinct role for Tyr residues in antimicrobial peptides.
Biochim Biophys Acta Biomembr. 2020 Mar 03;:183260
Authors: Arias M, Aramini JM, Riopel ND, Vogel HJ
Abstract
Because of their potential as novel antibiotic agents, antimicrobial peptides (AMPs) have generated considerable interest. The...
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[NMR paper] Thiourea-Based Inhibitors of the B-Cell Lymphoma 6 (BCL6) BTB Domain via NMR-Based Fragment Screening and Computer-Aided Drug Design.
Thiourea-Based Inhibitors of the B-Cell Lymphoma 6 (BCL6) BTB Domain via NMR-Based Fragment Screening and Computer-Aided Drug Design.
Related Articles Thiourea-Based Inhibitors of the B-Cell Lymphoma 6 (BCL6) BTB Domain via NMR-Based Fragment Screening and Computer-Aided Drug Design.
J Med Chem. 2018 Jul 03;:
Authors: Cheng H, Linhares B, Yu W, Cardenas MG, Ai Y, Jiang W, Winkler A, Cohen S, Melnick A, MacKerell AD, Cierpicki T, Xue F
Abstract
Protein-protein interactions (PPI) between the transcriptional repressor B-cell...
Antimicrobial peptides and their superior fluorinated analogues: structure-activity relationships as revealed by NMR spectroscopy and MD calculations.
Antimicrobial peptides and their superior fluorinated analogues: structure-activity relationships as revealed by NMR spectroscopy and MD calculations.
Antimicrobial peptides and their superior fluorinated analogues: structure-activity relationships as revealed by NMR spectroscopy and MD calculations.
Chembiochem. 2010 Nov 22;11(17):2424-32
Authors: Díaz MD, Palomino-Schätzlein M, Corzana F, Andreu C, Carbajo RJ, del Olmo M, Canales-Mayordomo A, Pineda-Lucena A, Asensio G, Jiménez-Barbero J
The conformations of two synthetic pentapeptides with...
Design of Highly Fluorinated Peptides for Cell-based 19F NMR
Linear Mode
