NMR paper Design, expression and solid-state NMR characterization of silk-like materials constr

		BioNMR > Educational resources > Journal club
[NMR paper] Design, expression and solid-state NMR characterization of silk-like materials constr

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-25-2010, 08:21 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Design, expression and solid-state NMR characterization of silk-like materials constr

Design, expression and solid-state NMR characterization of silk-like materials constructed from sequences of spider silk, Samia cynthia ricini and Bombyx mori silk fibroins.

Related Articles Design, expression and solid-state NMR characterization of silk-like materials constructed from sequences of spider silk, Samia cynthia ricini and Bombyx mori silk fibroins.

J Biochem. 2005 Jun;137(6):721-9

Authors: Yang M, Asakura T

Silk has a long history of use in medicine as sutures. To address the requirements of a mechanically robust and biocompatible material, basic research to clarify the role of repeated sequences in silk fibroin in its structures and properties seems important as well as the development of a processing technique suitable for the preparation of fibers with excellent mechanical properties. In this study, three silk-like protein analogs were constructed from two regions selected from among the crystalline region of Bombyx mori silk fibroin, (GAGSGA)(2), the crystalline region of Samia cynthia ricini silk fibroin, (Ala)(12), the crystalline region of spider dragline silk fibroin, (Ala)(6), and the Gly-rich region of spider silk fibroin, (GGA)(4). The silk-like protein analog constructed from the crystalline regions of the spider dragline silk and B. mori silk fibroins, (A(6)SCS)(8), that constructed from the crystalline regions of the S. c.ricini and B. mori silk fibroins, (A(12)SGS)(4), that constructed from and the crystalline region of S. c.ricini silk fibroin and the glycine-rich region of spider dragline silk fibroin, (A(12)SGS)(4),were expressed their molecular weights being about 36.0 kDa, 17.0 kDa and 17.5 kDa, respectively in E. coli by means of genetic engineering technologies. (A(12)SCS)(4) and (A(12)SGS)(4 )undergo a structural transition from alpha-helix to beta-sheet on a change in the solvent treatment from trifluoroacetic acid (TFA) to formic acid (FA). However, (A(6)SCS)(8) takes on the beta-sheet structure predominantly on TFA treatment and FA treatment. Structural analysis was performed on model peptides selected from spider dragline and S. c.ricini silks by means of (13)C CP/MAS NMR.

PMID: 16002994 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR tweet] Multinuclear Solid-State Nuclear Magnetic Resonance of Inorganic Materials, Volume 6 (Pergamon Materials Series): http://amzn.to/kSPkSn Multinuclear Solid-State Nuclear Magnetic Resonance of Inorganic Materials, Volume 6 (Pergamon Materials Series): http://amzn.to/kSPkSn Published by Carolazkl (Carola Dundlow) on 2011-05-22T01:28:17Z Source: Twitter	nmrlearner	Twitter NMR	0	05-22-2011 01:54 AM
[NMR tweet] Multinuclear Solid-State Nuclear Magnetic Resonance of Inorganic Materials, Volume 6 (Pergamon Materials Series): http://amzn.to/kSPkSn Multinuclear Solid-State Nuclear Magnetic Resonance of Inorganic Materials, Volume 6 (Pergamon Materials Series): http://amzn.to/kSPkSn Published by Lucilleqzkw (Lucille Guardiola) on 2011-05-18T09:25:57Z Source: Twitter	nmrlearner	Twitter NMR	0	05-18-2011 09:27 AM
[NMR tweet] Multinuclear Solid-State Nuclear Magnetic Resonance of Inorganic Materials, Volume 6 (Pergamon Materials Series): http://amzn.to/ewA2OW Multinuclear Solid-State Nuclear Magnetic Resonance of Inorganic Materials, Volume 6 (Pergamon Materials Series): http://amzn.to/ewA2OW Published by Simonago63 (Simona Likes) on 2011-04-25T19:18:52Z Source: Twitter	nmrlearner	Twitter NMR	0	04-25-2011 07:21 PM
Solid-state NMR evidence for elastin-like beta-turn structure in spider dragline silk. Solid-state NMR evidence for elastin-like beta-turn structure in spider dragline silk. Solid-state NMR evidence for elastin-like beta-turn structure in spider dragline silk. Chem Commun (Camb). 2010 Sep 28;46(36):6714-6 Authors: Jenkins JE, Creager MS, Butler EB, Lewis RV, Yarger JL, Holland GP Two-dimensional homo- and heteronuclear solid-state MAS NMR experiments on (13)C/(15)N-proline labeled Argiope aurantia dragline silk provide evidence for an elastin-like beta-turn structure for the repetitive Gly-Pro-Gly-X-X motif prevalent in major...	nmrlearner	Journal club	0	12-28-2010 03:31 PM
[NMR paper] Structural role of tyrosine in Bombyx mori silk fibroin, studied by solid-state NMR a Structural role of tyrosine in Bombyx mori silk fibroin, studied by solid-state NMR and molecular mechanics on a model peptide prepared as silk I and II. Related Articles Structural role of tyrosine in Bombyx mori silk fibroin, studied by solid-state NMR and molecular mechanics on a model peptide prepared as silk I and II. Magn Reson Chem. 2004 Feb;42(2):258-66 Authors: Asakura T, Suita K, Kameda T, Afonin S, Ulrich AS The influence of the bulky and H-bonding Tyr side-chain on its Ala- and Gly-rich environment in Bombyx mori silk fibroin was...	nmrlearner	Journal club	0	11-24-2010 09:25 PM
[NMR paper] Design, high-level expression, purification and characterization of soluble fragments Design, high-level expression, purification and characterization of soluble fragments of the hepatitis C virus NS3 RNA helicase suitable for NMR-based drug discovery methods and mechanistic studies. Related Articles Design, high-level expression, purification and characterization of soluble fragments of the hepatitis C virus NS3 RNA helicase suitable for NMR-based drug discovery methods and mechanistic studies. Protein Eng. 2001 Aug;14(8):573-82 Authors: Gesell JJ, Liu D, Madison VS, Hesson T, Wang YS, Weber PC, Wyss DF RNA helicases represent...	nmrlearner	Journal club	0	11-19-2010 08:44 PM
[NMR paper] Solid-state NMR determination of the secondary structure of Samia cynthia ricini silk Solid-state NMR determination of the secondary structure of Samia cynthia ricini silk. Related Articles Solid-state NMR determination of the secondary structure of Samia cynthia ricini silk. Nature. 2000 Jun 29;405(6790):1077-9 Authors: van Beek JD, Beaulieu L, Schäfer H, Demura M, Asakura T, Meier BH Silks are fibrous proteins that form heterogeneous, semi-crystalline solids. Silk proteins have a variety of physical properties reflecting their range of functions. Spider dragline silk, for example, has high tensile strength and elasticity,...	nmrlearner	Journal club	0	11-18-2010 09:15 PM
[NMR paper] Supercontracted spider dragline silk: a solid-state NMR study of the local structure. Supercontracted spider dragline silk: a solid-state NMR study of the local structure. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Supercontracted spider dragline silk: a solid-state NMR study of the local structure. Int J Biol Macromol. 1999 Mar-Apr;24(2-3):173-8 Authors: van Beek JD, KÃ¼mmerlen J, Vollrath F, Meier BH The local structure of supercontracted dragline silk from the spider Nephila madagascariensis was investigated by solid-state nuclear magnetic...	nmrlearner	Journal club	0	08-21-2010 04:03 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off