Publication year: 2011 Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 10 March 2011
Matthias J.N., Junk , Hans W., Spiess , Dariush, Hinderberger
In this study, self-assembled systems of human serum albumin (HSA) and spin-labeled fatty acids are characterized by double electron–electron resonance (DEER). HSA, being the most important transport protein of the human blood, is capable to host up to seven paramagnetic fatty acid derivatives. DEER measurements of these self-assembled multispin clusters are strongly affected by correlations of more than two spins, the evaluation of the latter constituting the central topic of this paper. While the DEER modulation depth can be used to obtain qualitative information of the number of coupled spins, the quantitative analysis is hampered by the occurrence of cluster... Graphical abstract
*Graphical abstract:**Highlights:*? Human serum albumin (HSA) and spin-labeled fatty acids (FA) self assemble in solution. ? DEER measurements on HSA are strongly affected by multispin correlations. ? With flip angle dependent DEER measurements we quantify multispin correlations. ? small distances are overestimated and large distances are suppressed. ? It is favorable to use spin-diluted systems for a quantitative analysis.
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Binding of the medium-chain fatty acids (MCFA), octanoic (OCT) and decanoic (DEC) acid, to human serum albumin (HSA) has been studied by 13C NMR spectroscopy. NMR spectra at 35 degrees C showed an apparently homogeneous binding environment (a single, narrow resonance for the 13C-enriched carboxyl...
DEER in Biological Multispin-Systems: A Case Study on the Fatty Acid Binding to Human Serum Albumin
Linear Mode
