Authors: Khan NS, Riggle BA, Seward GK, Bai Y, Dmochowski IJ
Abstract
Folate-conjugated cryptophane was developed for targeting cryptophane to membrane-bound folate receptors that are overexpressed in many human cancers. The cryptophane biosensor was synthesized in 20 nonlinear steps, which included functionalization with folate recognition moiety, solubilizing peptide, and Cy3 fluorophore. Hyperpolarized (129)Xe NMR studies confirmed xenon binding to the folate-conjugated cryptophane. Cellular internalization of biosensor was monitored by confocal laser scanning microscopy and quantified by flow cytometry. Competitive blocking studies confirmed cryptophane endocytosis through a folate receptor-mediated pathway. Flow cytometry revealed 10-fold higher cellular internalization in KB cancer cells overexpressing folate receptors compared to HT-1080 cells with normal folate receptor expression. The biosensor was determined to be nontoxic in HT-1080 and KB cells by MTT assay at low micromolar concentrations typically used for hyperpolarized (129)Xe NMR experiments.
A "Smart" (129)Xe NMR Biosensor for pH-Dependent Cell Labeling
From The DNP-NMR Blog:
A "Smart" (129)Xe NMR Biosensor for pH-Dependent Cell Labeling
Riggle BA, Wang Y, Dmochowski IJ. A "Smart" (129)Xe NMR Biosensor for pH-Dependent Cell Labeling. J Am Chem Soc. 2015;137(16):5542-8.
http://www.ncbi.nlm.nih.gov/pubmed/25848822
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06-01-2015 03:02 PM
[NMR paper] Cell-compatible, integrin-targeted cryptophane-(129)Xe NMR biosensors.
Cell-compatible, integrin-targeted cryptophane-(129)Xe NMR biosensors.
Related Articles Cell-compatible, integrin-targeted cryptophane-(129)Xe NMR biosensors.
Chem Sci. 2011 Jun;2(6):1103-1110
Authors: Seward GK, Bai Y, Khan NS, Dmochowski IJ
Abstract
Peptide-modified cryptophane enables sensitive detection of protein analytes using hyperpolarized (129)Xe NMR spectroscopy. Here we report improved targeting and delivery of cryptophane to cells expressing ?v?3 integrin receptor, which is overexpressed in many human cancers....
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11-06-2014 08:45 PM
Modular 129Xe NMR biosensor for MRI applications [Biophysics and Computational Biology]
Modular 129Xe NMR biosensor for MRI applications
Rose, H. M., Witte, C., Rossella, F., Klippel, S., Freund, C., Schroder, L....
Date: 2014-08-12
Magnetic resonance imaging (MRI) is seriously limited when aiming for visualization of targeted contrast agents. Images are reconstructed from the weak diamagnetic properties of the sample and require an abundant molecule like water as the reporter. Micromolar to millimolar concentrations of conventional contrast agents are needed to generate image contrast,... Read More
PNAS:
Number: 32
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08-13-2014 07:49 AM
[NMR paper] Development of an antibody-based, modular biosensor for 129Xe NMR molecular imaging of cells at nanomolar concentrations.
Development of an antibody-based, modular biosensor for 129Xe NMR molecular imaging of cells at nanomolar concentrations.
Development of an antibody-based, modular biosensor for 129Xe NMR molecular imaging of cells at nanomolar concentrations.
Proc Natl Acad Sci U S A. 2014 Jul 28;
Authors: Rose HM, Witte C, Rossella F, Klippel S, Freund C, Schröder L
Abstract
Magnetic resonance imaging (MRI) is seriously limited when aiming for visualization of targeted contrast agents. Images are reconstructed from the weak diamagnetic...
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07-30-2014 10:22 AM
[NMR paper] Molecular imaging of cancer cells using a bacteriophage-based 129Xe NMR biosensor.
Molecular imaging of cancer cells using a bacteriophage-based 129Xe NMR biosensor.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles Molecular imaging of cancer cells using a bacteriophage-based 129Xe NMR biosensor.
Angew Chem Int Ed Engl. 2013 Apr 26;52(18):4849-53
Authors: Palaniappan KK, Ramirez RM, Bajaj VS, Wemmer DE, Pines A, Francis MB
PMID: 23554263
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10-19-2013 03:22 PM
[NMR paper] High pressure NMR reveals active-site hinge motion of folate-bound Escherichia coli d
High pressure NMR reveals active-site hinge motion of folate-bound Escherichia coli dihydrofolate reductase.
Related Articles High pressure NMR reveals active-site hinge motion of folate-bound Escherichia coli dihydrofolate reductase.
Biochemistry. 2000 Oct 24;39(42):12789-95
Authors: Kitahara R, Sareth S, Yamada H, Ohmae E, Gekko K, Akasaka K
A high-pressure (15)N/(1)H two-dimensional NMR study has been carried out on folate-bound dihydrofolate reductase (DHFR) from Escherichia coli in the pressure range between 30 and 2000 bar. Several...
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11-19-2010 08:29 PM
[NMR paper] 13C NMR determination of the tautomeric and ionization states of folate in its comple
13C NMR determination of the tautomeric and ionization states of folate in its complexes with Lactobacillus casei dihydrofolate reductase.
Related Articles 13C NMR determination of the tautomeric and ionization states of folate in its complexes with Lactobacillus casei dihydrofolate reductase.
Biochemistry. 1993 Jul 13;32(27):6846-54
Authors: Cheung HT, Birdsall B, Frenkiel TA, Chau DD, Feeney J
13C NMR studies provide a convenient way of obtaining detailed information about tautomeric and ionization states in protein-ligand complexes provided...
Cryptophane-folate biosensor for (129)xe NMR.
Linear Mode
