Magic-angle spinning (MAS) solid-state nuclear magnetic resonance (SSNMR) spectroscopy is a powerful and versatile technique for probing structure and dynamics in large, insoluble biological systems at atomic resolution. With many recent advances in instrumentation and polarization methods, technology development in SSNMR remains an active area of research and presents opportunities to further improve data collection, processing, and analysis of samples with low sensitivity and complex tertiary...
Temperature dependence of cross-effect dynamic nuclear polarization in rotating solids: advantages of elevated temperatures #DNPNMR
From The DNP-NMR Blog:
Temperature dependence of cross-effect dynamic nuclear polarization in rotating solids: advantages of elevated temperatures #DNPNMR
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Geiger, M.A., et al., Temperature dependence of cross-effect dynamic nuclear polarization in rotating solids: advantages of elevated temperatures. Phys. Chem. Chem. Phys., 2016. 18(44): p. 30696-30704.
https://www.ncbi.nlm.nih.gov/pubmed/27791210
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01-25-2017 11:13 PM
[U. of Ottawa NMR Facility Blog] Cross Polarization Based Mixture Resolution in Solids
Cross Polarization Based Mixture Resolution in Solids
One of the most common techniques used to collect solid-state NMR data for spin I = ½ nuclides is a combination of cross polarization (CP) and magic angle spinning (MAS). CPMAS provides high sensitivity from the CP and high chemical shift resolution from the MAS. Furthermore, the scan repetition rate depends on the shorter relaxation time of the protons rather than the longer relaxation time of the spin I = ½ nuclide therefore, more scans can be collected per unit time. It must be remembered however, that the success of the CP...
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03-31-2015 07:17 PM
[U. of Ottawa NMR Facility Blog] Measurement of 13C 90° Pulses in Solids via Cross Polarization
Measurement of 13C 90° Pulses in Solids via Cross Polarization
The direct measurement of 13C 90° pulses in solids under MAS conditions by the conventional method suffers from the very low inherent sensitivity of 13C and is very time consuming due to the typically long 13C T1's. These problems can be at least partially overcome by using 1H - 13C cross polarization which has a potential four-fold sensitivity gain and also a time advantage as the repetition rate depends on the 1H T1 rather than the 13C T1, the former typically being less than the latter by a factor of ten. The 90° pulses...
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02-21-2014 08:51 PM
[NMR paper] Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids.
Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids.
J Magn Reson. 2013 Jun;231:5-14
Authors: Potapov A, Yau WM, Tycko R
Abstract
We explore the possibility of using dynamic nuclear polarization (DNP) to enhance signals in structural studies of biological solids by solid state NMR...
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01-11-2014 07:50 PM
Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids
From The DNP-NMR Blog:
Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids
Potapov, A., W.M. Yau, and R. Tycko, Dynamic nuclear polarization-enhanced (13)C NMR spectroscopy of static biological solids. J Magn Reson, 2013. 231(0): p. 5-14.
http://www.ncbi.nlm.nih.gov/pubmed/23562665
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06-25-2013 12:17 AM
Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids
Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids
Available online 27 February 2013
Publication year: 2013
Source:Journal of Magnetic Resonance</br>
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We explore the possibility of using dynamic nuclear polarization (DNP) to enhance signals in structural studies of biological solids by solid state NMR without sample spinning. Specifically, we use 2D 13C-13C exchange spectroscopy to probe the peptide backbone torsion angles (?,?) in a series of selectively 13C-labeled 40-residue ?-amyloid (A ?1-40) samples, in both fibrillar and...
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02-28-2013 07:57 PM
Multidimensional Magic Angle Spinning NMR Spectroscopy for Site-Resolved Measurement of Proton Chemical Shift Anisotropy in Biological Solids
Multidimensional Magic Angle Spinning NMR Spectroscopy for Site-Resolved Measurement of Proton Chemical Shift Anisotropy in Biological Solids
Guangjin Hou, Sivakumar Paramasivam, Si Yan, Tatyana Polenova and Alexander J. Vega
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja3084972/aop/images/medium/ja-2012-084972_0008.gif
Journal of the American Chemical Society
DOI: 10.1021/ja3084972
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/y3Jt7S8MwHM
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01-22-2013 09:14 PM
Ergodicity and efficiency of cross-polarization in NMR of static solids.
Ergodicity and efficiency of cross-polarization in NMR of static solids.
Ergodicity and efficiency of cross-polarization in NMR of static solids.
J Magn Reson. 2011 Apr;209(2):161-6
Authors: Nevzorov AA
Cross-polarization transfer is employed in virtually every solid-state NMR experiment to enhance magnetization of low-gamma spins. Theory and experiment is used to assess the magnitude of the final quasistationary magnetization amplitude. The many-body density matrix equation is solved for relatively large (up to N=14) spin systems without the...
Cross polarization stability in multidimensional NMR spectroscopy of biological solids
Linear Mode
