Adiabatically swept pulses were originally designed for the purpose of broadband spin inversion. Later, unexpected advantages of their utilization were also found in other applications, such as refocusing to excite spin echoes, studies of chemical exchange or fragment-based drug design. Here, we present new experiments to characterize fast (psâ??ns) protein dynamics, which benefit from little-known properties of adiabatic pulses. We developed a strategy for measuring cross-correlated cross-relaxation (CCCR) rates during adiabatic pulses. This experiment provides a linear combination of longitudinal and transverse CCCR rates, which is offset-independent across a typical amide \(^{15}\hbox {N}\) spectrum. The pulse sequence can be recast to provide accurate transverse CCCR rates weighted by the populations of exchanging states. Sensitivity can be improved in systems in slow exchange. Finally, the experiments can be easily modified to yield residue-specific correlation times. The average correlation time of motions can be determined with a single experiment while at least two different experiments had to be recorded until now.
[NMR paper] Anisotropy of Rotational Diffusion, Dipole-Dipole Cross-Correlated NMR Relaxation and Angles between Bond Vectors in Proteins.
Anisotropy of Rotational Diffusion, Dipole-Dipole Cross-Correlated NMR Relaxation and Angles between Bond Vectors in Proteins.
Related Articles Anisotropy of Rotational Diffusion, Dipole-Dipole Cross-Correlated NMR Relaxation and Angles between Bond Vectors in Proteins.
Chemphyschem. 2001 Sep 17;2(8-9):539-43
Authors: Deschamps M, Bodenhausen G
Abstract
Cross correlations between the fluctuations of dipolar (13) C(?) -(1) H(?) interactions yield information about the relative orientation of successive (13) C(?) -(1) H(?) bond vectors...
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[NMR paper] Probing Local Backbone Geometries in Intrinsically Disordered Proteins by Cross-Correlated NMR Relaxation.
Probing Local Backbone Geometries in Intrinsically Disordered Proteins by Cross-Correlated NMR Relaxation.
Probing Local Backbone Geometries in Intrinsically Disordered Proteins by Cross-Correlated NMR Relaxation.
Angew Chem Int Ed Engl. 2013 Mar 20;
Authors: Stanek J, Saxena S, Geist L, Konrat R, Ko?mi?ski W
Abstract
Ab ultra-high-resolution NMR experiment for the measurement of intraresidue (1) H(i)-(15) N(i)-(13) C'(i) dipolar-chemical shift anisotropy relaxation interference is employed to extract information about local backbone...
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Full relaxation matrix analysis of apparent cross-correlated relaxation rates in four-spin systems
Full relaxation matrix analysis of apparent cross-correlated relaxation rates in four-spin systems
January 2013
Publication year: 2013
Source:Journal of Magnetic Resonance, Volume 226</br>
</br>
Cross-correlated relaxation (CCR) rates are an established tool for the extraction of relative bond orientations in biomolecules in solution. CCR between dipolar interactions in four-spin systems is a particularly well-suited mechanism. In this paper, a simple approach to analyze systematic experimental errors is formulated in a subspace of the complete four-spin Hilbert space....
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Full relaxation matrix analysis of apparent cross-correlated relaxation rates in four-spin systems
Full relaxation matrix analysis of apparent cross-correlated relaxation rates in four-spin systems
Available online 12 November 2012
Publication year: 2012
Source:Journal of Magnetic Resonance</br>
</br>
Cross-correlated relaxation (CCR) rates are an established tool for the extraction of relative bond orientations in biomolecules in solution. CCR between dipolar interactions in four-spin systems is a particularly well-suited mechanism. In this paper, a simple approach to analyze systematic experimental errors is formulated in a subspace of the complete four-spin...
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12-01-2012 06:10 PM
Full relaxation matrix analysis of apparent cross-correlated relaxation rates in four-spin systems
Full relaxation matrix analysis of apparent cross-correlated relaxation rates in four-spin systems
Publication year: 2012
Source:Journal of Magnetic Resonance</br>
Beat Vögeli</br>
Cross-correlated relaxation (CCR) rates are an established tool for the extraction of relative bond orientations in biomolecules in solution. CCR between dipolar interactions in four-spin systems is a particularly well-suited mechanism. In this paper, a simple approach to analyze systematic experimental errors is formulated in a subspace of the complete four-spin Hilbert space. It is shown...
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11-13-2012 07:30 AM
NMR Cross-Correlated Relaxation Rates Reveal Ion Coordination Sites in DNA
NMR Cross-Correlated Relaxation Rates Reveal Ion Coordination Sites in DNA
Radovan Fiala, Nad?a S?pac?kova?, Silvie Foldynova?-Tranti?rkova?, Jir?i? S?poner, Vladimi?r Sklena?r? and Luka?s? Tranti?rek
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja202397p/aop/images/medium/ja-2011-02397p_0003.gif
Journal of the American Chemical Society
DOI: 10.1021/ja202397p
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/vJtIag8UbVQ
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[NMR paper] Automated NMR determination of protein backbone dihedral angles from cross-correlated
Automated NMR determination of protein backbone dihedral angles from cross-correlated spin relaxation.
Related Articles Automated NMR determination of protein backbone dihedral angles from cross-correlated spin relaxation.
J Biomol NMR. 2002 Apr;22(4):349-63
Authors: Kloiber K, Schüler W, Konrat R
The simultaneous interpretation of a suite of dipole-dipole and dipole-CSA cross-correlation rates involving the backbone nuclei 13Calpha, 1Halpha, 13CO, 15N and 1HN can be used to resolve the ambiguities associated with each individual...
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[NMR paper] Polarization transfer by cross-correlated relaxation in solution NMR with very large
Polarization transfer by cross-correlated relaxation in solution NMR with very large molecules.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-custom-pnas_full_free.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Polarization transfer by cross-correlated relaxation in solution NMR with very large molecules.
Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):4918-23
Authors: Riek R, Wider G, Pervushin K, Wüthrich...