Publication year: 2012 Source: Journal of Magnetic Resonance, Available online 6 March 2012
Yixuan*Li, Qiang*Wang, Zhengfeng*Zhang, Jun*Yang, Bingwen*Hu, ...
Two-dimensional covariance (COV2D) spectroscopy with non-uniform and consecutive acquisition (NUCA) scheme is introduced. This NUCA-COV2D method allows the number of t1points to be reduced by a factor of 1.5~3 without any broadening of the linewidth. Furthermore, the signal-to-noise ratio (S/N) can be increased up to 50%, which can further save experimental time by another factor of 2. This method has been demonstrated with model samples and the microcrystalline proteins. In all cases, the total experimental time can be reduced by a factor of 3~6, without any loss of resolution and S/N, with respect to what is necessary with the FT2D NMR. Graphical abstract
Highlights
? Compared with UCA-FT2D, NUCA-COV2D achieves better resolution and saves considerable experimental time. ? Compared with NUCA-FT2D, NUCA-COV2D achieves better S/N and resolution and saves considerable experimental time. ? Compared with UCA-COV2D, NUCA-COV2D increases the signal-to-noise ratio (S/N) by a factor of 2.
Signal enhancement for the sensitivity-limited solid state NMR experiments using a continuous, non-uniform acquisition scheme.
Signal enhancement for the sensitivity-limited solid state NMR experiments using a continuous, non-uniform acquisition scheme.
Signal enhancement for the sensitivity-limited solid state NMR experiments using a continuous, non-uniform acquisition scheme.
J Magn Reson. 2011 Aug 30;
Authors: Qiang W
Abstract
We describe a sampling scheme for the two-dimensional (2D) solid state NMR experiments, which can be readily applied to the sensitivity-limited samples. The sampling scheme utilizes continuous, non-uniform sampling profile for the...
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09-21-2011 03:31 PM
DNP by Thermal Mixing under Optimized Conditions Yields >60 000-fold Enhancement of 89Y NMR Signal
DNP by Thermal Mixing under Optimized Conditions Yields >60 000-fold Enhancement of 89Y NMR Signal
Lloyd Lumata, Ashish K. Jindal, Matthew E. Merritt, Craig R. Malloy, A. Dean Sherry and Zoltan Kovacs
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja201880y/aop/images/medium/ja-2011-01880y_0010.gif
Journal of the American Chemical Society
DOI: 10.1021/ja201880y
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/lZjUD7bs_fI
Rapid acquisition of (1) H and (19) F NMR experiments for direct and competition ligand-based screening.
Rapid acquisition of (1) H and (19) F NMR experiments for direct and competition ligand-based screening.
Rapid acquisition of (1) H and (19) F NMR experiments for direct and competition ligand-based screening.
Magn Reson Chem. 2011 Mar 9;
Authors: Dalvit C, Gossert AD, Coutant J, Piotto M
Direct and competition ligand-based NMR experiments are often used in the screening of chemical fragment libraries against a protein target due to the high relative sensitivity of NMR for protein-binding events. A plethora of NMR methods has been proposed...
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03-10-2011 03:51 PM
Very simple combination of TROSY, CRINEPT and multiple quantum coherence for signal enhancement in an HN(CO)CA experiment for large proteins
Very simple combination of TROSY, CRINEPT and multiple quantum coherence for signal enhancement in an HN(CO)CA experiment for large proteins
Publication year: 2011
Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 3 February 2011</br>
Monika, Bayrhuber , Roland, Riek</br>
Sensitivity enhancement in liquid state nuclear magnetic resonance (NMR) triple resonance experiments for the sequential assignment of proteins is important for the investigation of large proteins or protein complexes. We present here the 3D TROSY-MQ/CRINEPT-HN(CO)CA which makes...
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02-04-2011 07:03 AM
Signal enhancement in protein NMR using the spin-noise tuning optimum
Signal enhancement in protein NMR using the spin-noise tuning optimum
Abstract We have assessed the potential of an alternative probe tuning strategy based on the spin-noise response for application in common high-resolution multi-dimensional biomolecular NMR experiments with water signal suppression on aqueous and salty samples. The method requires the adjustment of the optimal tuning condition, which may be offset by several 100 kHz from the conventional tuning settings using the noise response of the water protons as an indicator. Although the radio frequency-pulse durations are...
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10-09-2010 03:03 AM
Signal enhancement in protein NMR using the spin-noise tuning optimum.
Signal enhancement in protein NMR using the spin-noise tuning optimum.
Signal enhancement in protein NMR using the spin-noise tuning optimum.
J Biomol NMR. 2010 Oct 6;
Authors: Nausner M, Goger M, Bendet-Taicher E, Schlagnitweit J, Jerschow A, Müller N
We have assessed the potential of an alternative probe tuning strategy based on the spin-noise response for application in common high-resolution multi-dimensional biomolecular NMR experiments with water signal suppression on aqueous and salty samples. The method requires the adjustment of the...
Covariance spectroscopy with a non-uniform and consecutive acquisition scheme for signal enhancement of the NMR experiments
Linear Mode
