NMR paper Covariance spectroscopy in high-resolution multi-dimensional solid-state NMR.

		BioNMR > Educational resources > Journal club
[NMR paper] Covariance spectroscopy in high-resolution multi-dimensional solid-state NMR.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

10-07-2014, 02:31 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Covariance spectroscopy in high-resolution multi-dimensional solid-state NMR.

Related Articles Covariance spectroscopy in high-resolution multi-dimensional solid-state NMR.

J Magn Reson. 2014 Feb;239:57-60

Authors: Lin EC, Opella SJ

Abstract
Covariance spectroscopy (COV), a statistical method that provides increased sensitivity, can be applied to two-dimensional high-resolution solid-state NMR experiments, such as homonuclear spin-exchange spectroscopy. We the alternative States sampling scheme to the experimental time by 50%. By combining COV with other processing methods for non-uniform sampling (NUS), many different three-dimensional experiments can be performed with substantial increases in overall sensitivity. As an example, we show a three-dimensional homonuclear spin-exchange/separated-local-field (SLF) spectrum that enables the assignment of resonances and the measurement of structural restraints from a single experiment performed in a limited amount of time.

PMID: 24380813 [PubMed - indexed for MEDLINE]

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Accurate measurements of (13)C-(13)C distances in uniformly (13)C-labeled proteins using multi-dimensional four-oscillating field solid-state NMR spectroscopy. Accurate measurements of (13)C-(13)C distances in uniformly (13)C-labeled proteins using multi-dimensional four-oscillating field solid-state NMR spectroscopy. Related Articles Accurate measurements of (13)C-(13)C distances in uniformly (13)C-labeled proteins using multi-dimensional four-oscillating field solid-state NMR spectroscopy. J Chem Phys. 2014 Sep 21;141(11):114201 Authors: Straasø LA, Nielsen JT, Bjerring M, Khaneja N, Nielsen NC Abstract Application of sets of (13)C-(13)C internuclear distance restraints...	nmrlearner	Journal club	0	09-23-2014 01:57 PM
[NMR paper] High-resolution heteronuclear multi-dimensional NMR spectroscopy in magnetic fields with unknown spatial variations. High-resolution heteronuclear multi-dimensional NMR spectroscopy in magnetic fields with unknown spatial variations. Related Articles High-resolution heteronuclear multi-dimensional NMR spectroscopy in magnetic fields with unknown spatial variations. J Magn Reson. 2014 Feb 20;242C:49-56 Authors: Zhang Z, Huang Y, Smith PE, Wang K, Cai S, Chen Z Abstract Heteronuclear NMR spectroscopy is an extremely powerful tool for determining the structures of organic molecules and is of particular significance in the structural analysis of proteins. In...	nmrlearner	Journal club	0	03-14-2014 06:40 AM
High-resolution heteronuclear multi-dimensional NMR spectroscopy in magnetic fields with unknown spatial variations High-resolution heteronuclear multi-dimensional NMR spectroscopy in magnetic fields with unknown spatial variations Publication date: Available online 20 February 2014 Source:Journal of Magnetic Resonance</br> Author(s): Zhiyong Zhang , Yuqing Huang , Pieter E.S. Smith , Kaiyu Wang , Shuhui Cai , Zhong Chen</br> Heteronuclear NMR spectroscopy is an extremely powerful tool for determining the structures of organic molecules and is of particular significance in the structural analysis of proteins. In order to leverage the method’s potential for structural...	nmrlearner	Journal club	0	02-20-2014 05:15 AM
Three-dimensional deuterium-carbon correlation experiments for high-resolution solid-state MAS NMR spectroscopy of large proteins Three-dimensional deuterium-carbon correlation experiments for high-resolution solid-state MAS NMR spectroscopy of large proteins Abstract Well-resolved 2Hâ??13C correlation spectra, reminiscent of 1Hâ??13C correlations, are obtained for perdeuterated ubiquitin and for perdeuterated outer-membrane protein G (OmpG) from E. coli by exploiting the favorable lifetime of 2H double-quantum (DQ) states. Sufficient signal-to-noise was achieved due to the short deuterium T 1, allowing for high repetition rates and enabling 3D experiments with a 2Hâ??13C transfer step in a reasonable time....	nmrlearner	Journal club	0	11-01-2011 01:52 AM
Atomic-resolution three-dimensional structure of HET-s(218-289) amyloid fibrils by solid-state NMR spectroscopy. Atomic-resolution three-dimensional structure of HET-s(218-289) amyloid fibrils by solid-state NMR spectroscopy. Atomic-resolution three-dimensional structure of HET-s(218-289) amyloid fibrils by solid-state NMR spectroscopy. J Am Chem Soc. 2010 Oct 6;132(39):13765-75 Authors: Van Melckebeke H, Wasmer C, Lange A, Ab E, Loquet A, Böckmann A, Meier BH We present a strategy to solve the high-resolution structure of amyloid fibrils by solid-state NMR and use it to determine the atomic-resolution structure of the prion domain of the fungal prion HET-s...	nmrlearner	Journal club	0	01-21-2011 12:00 PM
[NMR paper] Three-dimensional solid-state NMR spectroscopy is essential for resolution of resonan Three-dimensional solid-state NMR spectroscopy is essential for resolution of resonances from in-plane residues in uniformly (15)N-labeled helical membrane proteins in oriented lipid bilayers. Related Articles Three-dimensional solid-state NMR spectroscopy is essential for resolution of resonances from in-plane residues in uniformly (15)N-labeled helical membrane proteins in oriented lipid bilayers. J Magn Reson. 2000 May;144(1):156-61 Authors: Marassi FM, Ma C, Gesell JJ, Opella SJ Uniformly (15)N-labeled samples of membrane proteins with...	nmrlearner	Journal club	0	11-18-2010 09:15 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off