Correlating Lipid Bilayer Fluidity with Sensitivity and Resolution of Polytopic Membrane Protein Spectra by Solid-State NMR Spectroscopy

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Correlating Lipid Bilayer Fluidity with Sensitivity and Resolution of Polytopic Membrane Protein Spectra by Solid-State NMR Spectroscopy

Publication date: Available online 13 May 2014
Source:Biochimica et Biophysica Acta (BBA) - Biomembranes

Author(s): James R. Banigan , Anindita Gayen , Nathaniel J. Traaseth

Solid-state NMR spectroscopy has emerged as an excellent tool to study the structure and dynamics of membrane proteins under native-like conditions in lipid bilayers. One of the key considerations in experimental design is the uniaxial rotational diffusion of the protein that can have a direct influence on the NMR spectral observables. In this regard, temperature plays a fundamental role in modulating the phase properties of the lipids, which directly influences the rotational diffusion rate of the protein in the bilayer. In fact, it is well established that below the main phase transition temperature of the lipid bilayer the protein’s motion is significantly slowed while above this critical temperature the rate is increased. In this article, we carried out a systematic comparison of the signal intensity and spectral resolution as a function of temperature using magic-angle-spinning (MAS) solid-state NMR spectroscopy. These observables were directly correlated with the relative fluidity of the lipid bilayer as inferred from differential scanning calorimetry (DSC). We applied our hybrid biophysical approach to polytopic membrane protein multidrug resistance transporters (EmrE and SugE) in the presence of model membrane lipid compositions (DMPC-14:0 and DPPC-16:0). From these experiments, we conclude that the rotational diffusion giving optimal spectral resolution corresponds to a bilayer fluidity of ~5%, which corresponds to the percentage of lipids in the fluid or liquid-crystalline fraction. At the temperature corresponding to this critical value of fluidity, there is sufficient mobility to reduce inhomogeneous line broadening that occurs at lower temperatures. A greater extent of fluidity leads to faster uniaxial rotational diffusion and a sigmoidal-type drop in the NMR signal intensity, which stems from intermediate-exchange dynamics where the motion has a similar frequency as the NMR observables (i.e., dipolar couplings and chemical shift anisotropy). These experiments provide insight into the optimal temperature ranges and corresponding bilayer fluidity to study membrane proteins by solid-state NMR spectroscopy. This article is part of a Special Issue entitled: NMR Spectroscopy for Atomistic Views of Biomembranes and Cell Surfaces.
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