Lipids adhere to membrane proteins to stimulate or suppress molecular and ionic transport and signal transduction. Yet, the molecular details of lipid-protein interaction and their functional impact are poorly characterized. Here we combine NMR, coarse-grained molecular dynamics (CGMD), and functional assays to reveal classic cooperativity in the binding and subsequent activation of a bacterial inward rectifier potassium (Kir) channel by phosphatidylglycerol (PG), a common component of many...
[NMR paper] Conformational equilibrium shift underlies altered K+ channel gating as revealed by NMR.
Conformational equilibrium shift underlies altered K+ channel gating as revealed by NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.nature.com-images-lo_npg.gif http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.nature.com-images-npg_logo.gif http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.png Related Articles Conformational equilibrium shift underlies altered K+ channel gating as revealed by NMR.
Nat Commun. 2020 10...
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[NMR paper] Site-specific resolution of anionic residues in proteins using solid-state NMR spectroscopy.
Site-specific resolution of anionic residues in proteins using solid-state NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Site-specific resolution of anionic residues in proteins using solid-state NMR spectroscopy.
J Biomol NMR. 2020 Jun 08;:
Authors: Li J, Sae Her A, Traaseth NJ
Abstract
NMR spectroscopy is commonly used to infer site-specific acid dissociation constants (pKa) since the chemical shift is...
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Site-specific resolution of anionic residues in proteins using solid-state NMR spectroscopy
Site-specific resolution of anionic residues in proteins using solid-state NMR spectroscopy
Abstract
NMR spectroscopy is commonly used to infer site-specific acid dissociation constants (pKa) since the chemical shift is sensitive to the protonation state. Methods that probe atoms nearest to the functional groups involved in acid/base chemistry are the most sensitive for determining the protonation state. In this work, we describe a magic-angle-spinning (MAS) solid-state NMR approach to measure chemical shifts on the side chain of the anionic residues...
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[NMR paper] Biomolecular complex viewed by dynamic nuclear polarization solid-state NMR spectroscopy.
Biomolecular complex viewed by dynamic nuclear polarization solid-state NMR spectroscopy.
Related Articles Biomolecular complex viewed by dynamic nuclear polarization solid-state NMR spectroscopy.
Biochem Soc Trans. 2020 May 07;:
Authors: Chakraborty A, Deligey F, Quach J, Mentink-Vigier F, Wang P, Wang T
Abstract
Solid-state nuclear magnetic resonance (ssNMR) is an indispensable tool for elucidating the structure and dynamics of insoluble and non-crystalline biomolecules. The recent advances in the sensitivity-enhancing...
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[ASAP] Gating Mechanism of Aquaporin Z in Synthetic Bilayers and Native Membranes Revealed by Solid-State NMR Spectroscopy
Gating Mechanism of Aquaporin Z in Synthetic Bilayers and Native Membranes Revealed by Solid-State NMR Spectroscopy
Yongxiang Zhao, Huayong Xie, Lili Wang, Yang Shen, Wei Chen, Benteng Song, Zhengfeng Zhang, Anmin Zheng, Qingsong Lin, Riqiang Fu, Jufang Wang, Jun Yang
https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.8b03446/20180611/images/medium/ja-2018-03446h_0009.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.8b03446
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...
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[NMR paper] Gating Mechanism of Aquaporin Z in Synthetic Bilayers and Native Membranes Revealed by Solid-state NMR Spectroscopy.
Gating Mechanism of Aquaporin Z in Synthetic Bilayers and Native Membranes Revealed by Solid-state NMR Spectroscopy.
Related Articles Gating Mechanism of Aquaporin Z in Synthetic Bilayers and Native Membranes Revealed by Solid-state NMR Spectroscopy.
J Am Chem Soc. 2018 May 25;:
Authors: Zhao Y, Xie H, Wang L, Shen Y, Chen W, Song B, Zhang Z, Zheng A, Lin Q, Fu R, Wang J, Yang J
Abstract
Aquaporin Z (AqpZ) is an integral membrane protein that facilitates transport of water across E. coli cells with a high rate. Previously, R189,...
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[NMR paper] pH-Dependent Conformation, Dynamics, and Aromatic Interaction of*the*Gating Tryptophan Residue of the Influenza M2 Proton Channel from*Solid-State NMR.
pH-Dependent Conformation, Dynamics, and Aromatic Interaction of*the*Gating Tryptophan Residue of the Influenza M2 Proton Channel from*Solid-State NMR.
Related Articles pH-Dependent Conformation, Dynamics, and Aromatic Interaction of*the*Gating Tryptophan Residue of the Influenza M2 Proton Channel from*Solid-State NMR.
Biophys J. 2013 Apr 16;104(8):1698-708
Authors: Williams JK, Zhang Y, Schmidt-Rohr K, Hong M
Abstract
The M2 protein of the influenza virus conducts protons into the virion under external acidic pH. The proton selectivity of...
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[NMR paper] Membrane structure and dynamics as viewed by solid-state NMR spectroscopy.
Membrane structure and dynamics as viewed by solid-state NMR spectroscopy.
Related Articles Membrane structure and dynamics as viewed by solid-state NMR spectroscopy.
Biophys Chem. 1997 Oct;68(1-3):233-41
Authors: Auger M
The purpose of the present study is the investigation of the structure and dynamics of biological membranes using solid-state nuclear magnetic resonance (NMR) spectroscopy. Two approaches are used in our laboratory. The first involves the measurement of high-resolution 13C and 1H spectra obtained by the magic angle spinning...
Cooperative Gating of a K+ Channel by Unmodified Biological Anionic Lipids Viewed by Solid-State NMR Spectroscopy
Linear Mode
