While low temperature NMR holds great promise for the analysis of unstable samples and for sensitizing NMR detection, spectral broadening in frozen protein samples is a common experimental challenge. One hypothesis explaining the additional linewidth is that a variety of conformations are in rapid equilibrium at room temperature and become frozen, creating an inhomogeneous distribution at cryogenic temperatures. Here we investigate conformational heterogeneity by measuring the backbone torsion...
[NMR paper] Conformational heterogeneity of Savinase from NMR, HDX-MS and X-ray diffraction analysis.
Conformational heterogeneity of Savinase from NMR, HDX-MS and X-ray diffraction analysis.
Related Articles Conformational heterogeneity of Savinase from NMR, HDX-MS and X-ray diffraction analysis.
PeerJ. 2020;8:e9408
Authors: Wu S, Nguyen TTTN, Moroz OV, Turkenburg JP, Nielsen JE, Wilson KS, Rand KD, Teilum K
Abstract
Background: Several examples have emerged of enzymes where slow conformational changes are of key importance for function and where low populated conformations in the resting enzyme resemble the conformations of...
nmrlearner
Journal club
0
07-04-2020 09:21 PM
Anisotropic longitudinal electronic relaxation affects DNP at cryogenic temperatures #DNPNMR
From The DNP-NMR Blog:
Anisotropic longitudinal electronic relaxation affects DNP at cryogenic temperatures #DNPNMR
p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica}
Weber, E.M.M., et al., Anisotropic longitudinal electronic relaxation affects DNP at cryogenic temperatures. Phys. Chem. Chem. Phys., 2017. 19(24): p. 16087-16094.
https://www.ncbi.nlm.nih.gov/pubmed/28598474
nmrlearner
News from NMR blogs
0
12-18-2017 11:19 PM
[NMR paper] NMR Elucidation of Monomer-dimer transition and Conformational heterogeneity in Histone-like DNA binding protein of Helicobacter pylori (Hup).
NMR Elucidation of Monomer-dimer transition and Conformational heterogeneity in Histone-like DNA binding protein of Helicobacter pylori (Hup).
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7315-19-Wiley_FullText_120x30_orange.png Related Articles NMR Elucidation of Monomer-dimer transition and Conformational heterogeneity in Histone-like DNA binding protein of Helicobacter pylori (Hup).
Magn Reson Chem. 2017 Dec 14;:
Authors: Jaiswal N, Raikwal N, Pandey H, Agarwal N, Arora A, Poluri KM, Kumar D
...
nmrlearner
Journal club
0
12-15-2017 09:07 PM
Anisotropic longitudinal electronic relaxation affects DNP at cryogenic temperatures #DNPNMR
From The DNP-NMR Blog:
Anisotropic longitudinal electronic relaxation affects DNP at cryogenic temperatures #DNPNMR
Anisotropic relaxation effects are well know and understood in EPR spectroscopy and have long served as measures to understand the motion (libration) of paramagnetic co-factors (quinones, nitroxide radicals etc.) in biological system. In this study the authors investigate the effect of anisotropic relaxation effects in DNP experiments.
To find more about anisotropic relaxation effects studied by EPR take a look at the work by Sergei Dzuba or the Eatons:
nmrlearner
News from NMR blogs
0
11-13-2017 03:03 PM
Phenylazide Hybrid-Silica - Polarization Platform for Dynamic Nuclear Polarization at Cryogenic Temperatures #DNPNMR
From The DNP-NMR Blog:
Phenylazide Hybrid-Silica - Polarization Platform for Dynamic Nuclear Polarization at Cryogenic Temperatures #DNPNMR
p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica}
Grüning, W.R., et al., Phenylazide Hybrid-Silica - Polarization Platform for Dynamic Nuclear Polarization at Cryogenic Temperatures. Helvetica Chimica Acta, 2016: p. n/a-n/a.
http://dx.doi.org/10.1002/hlca.201600122
nmrlearner
News from NMR blogs
0
11-19-2016 08:35 PM
Conformational Disorder of Membrane Peptides Investigated from Solid-State NMR Linewidths and Lineshapes.
Conformational Disorder of Membrane Peptides Investigated from Solid-State NMR Linewidths and Lineshapes.
Conformational Disorder of Membrane Peptides Investigated from Solid-State NMR Linewidths and Lineshapes.
J Phys Chem B. 2011 Aug 1;
Authors: Su Y, Hong M
A challenge in the application of solid-state NMR spectroscopy to membrane proteins and peptides is the relatively broad linewidths compared to solution NMR spectra. To understand the linewidth contributions to membrane protein spectra, we have measured the inhomogeneous and homogeneous...
nmrlearner
Journal club
0
08-03-2011 12:00 PM
[NMR paper] Conformational heterogeneity of transmembrane residues after the Schiff base reproton
Conformational heterogeneity of transmembrane residues after the Schiff base reprotonation of bacteriorhodopsin: 15N CPMAS NMR of D85N/T170C membranes.
Related Articles Conformational heterogeneity of transmembrane residues after the Schiff base reprotonation of bacteriorhodopsin: 15N CPMAS NMR of D85N/T170C membranes.
FEBS J. 2005 May;272(9):2152-64
Authors: Mason AJ, Turner GJ, Glaubitz C
bR, N-like and O-like intermediate states of methionine-labelled wild type and D85N/T170C bacteriorhodopsin were accumulated in native membranes by...
Contribution of protein conformational heterogeneity to NMR lineshapes at cryogenic temperatures
Linear Mode
