NMR paper Construct Phenylethanoid Glycosides Harnessing Biosynthetic Networks, Protein Engineering and One-Pot Multienzyme Cascades

		BioNMR > Educational resources > Journal club
[NMR paper] Construct Phenylethanoid Glycosides Harnessing Biosynthetic Networks, Protein Engineering and One-Pot Multienzyme Cascades

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

05-14-2024, 05:30 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Construct Phenylethanoid Glycosides Harnessing Biosynthetic Networks, Protein Engineering and One-Pot Multienzyme Cascades

Construct Phenylethanoid Glycosides Harnessing Biosynthetic Networks, Protein Engineering and One-Pot Multienzyme Cascades

Angewandte Chemie International Edition, EarlyView.

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Engineering Biologics toward Challenging Membrane Protein Targets - Genetic Engineering & Biotechnology News Engineering Biologics toward Challenging Membrane Protein Targets Genetic Engineering & Biotechnology News Engineering Biologics toward Challenging Membrane Protein Targets - Genetic Engineering & Biotechnology News More...	nmrlearner	Online News	0	08-02-2021 05:20 PM
[ASAP] Harnessing Environmental Ca2+ for Extracellular Protein Thermostabilization Harnessing Environmental Ca2+ for Extracellular Protein Thermostabilization https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.0c00449/20200924/images/medium/bi0c00449_0013.gif Biochemistry DOI: 10.1021/acs.biochem.0c00449 http://feeds.feedburner.com/~r/acs/bichaw/~4/fDgFR00dJQA More...	nmrlearner	Journal club	0	09-26-2020 07:23 AM
[NMR paper] Ordered phosphorylation events in two independent cascades of the PTEN C-tail revealed by NMR. Ordered phosphorylation events in two independent cascades of the PTEN C-tail revealed by NMR. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Ordered phosphorylation events in two independent cascades of the PTEN C-tail revealed by NMR. J Am Chem Soc. 2012 Dec 19;134(50):20533-43 Authors: Cordier F, Chaffotte A, Terrien E, Préhaud C, Theillet FX, Delepierre M, Lafon M, Buc H, Wolff N Abstract PTEN phosphatase is a tumor suppressor controlling notably cell...	nmrlearner	Journal club	0	06-07-2013 10:04 AM
Acid epimerization of 20-keto pregnane glycosides is determined by 2D-NMR spectroscopy Acid epimerization of 20-keto pregnane glycosides is determined by 2D-NMR spectroscopy Abstract Carbohydrates influence many essential biological events such as apoptosis, differentiation, tumor metastasis, cancer, neurobiology, immunology, development, host-pathogen interactions, diabetes, signal transduction, protein folding, and many other contexts. We now report on the structure determination of pregnane glycosides isolated from the aerial parts of Ceropegia fusca Bolle (Asclepiadaceae). The observation of cicatrizant, vulnerary and cytostatic activities in some humans and animals...	nmrlearner	Journal club	0	03-26-2011 08:35 PM
Acid epimerization of 20-keto pregnane glycosides is determined by 2D-NMR spectroscopy. Acid epimerization of 20-keto pregnane glycosides is determined by 2D-NMR spectroscopy. Acid epimerization of 20-keto pregnane glycosides is determined by 2D-NMR spectroscopy. J Biomol NMR. 2011 Mar 24; Authors: García VP Carbohydrates influence many essential biological events such as apoptosis, differentiation, tumor metastasis, cancer, neurobiology, immunology, development, host-pathogen interactions, diabetes, signal transduction, protein folding, and many other contexts. We now report on the structure determination of pregnane glycosides...	nmrlearner	Journal club	0	03-25-2011 06:34 PM
Internal and global protein motion assessed with a fusion construct and in-cell NMR spectroscopy. Internal and global protein motion assessed with a fusion construct and in-cell NMR spectroscopy. Internal and global protein motion assessed with a fusion construct and in-cell NMR spectroscopy. Chembiochem. 2011 Feb 11;12(3):390-1 Authors: Barnes CO, Monteith WB, Pielak GJ	nmrlearner	Journal club	0	02-04-2011 11:34 AM
Internal and Global Protein Motion Assessed with a Fusion Construct and In-Cell NMR Spectroscopy. Internal and Global Protein Motion Assessed with a Fusion Construct and In-Cell NMR Spectroscopy. Internal and Global Protein Motion Assessed with a Fusion Construct and In-Cell NMR Spectroscopy. Chembiochem. 2010 Dec 15; Authors: Barnes CO, Monteith WB, Pielak GJ	nmrlearner	Journal club	0	12-17-2010 11:23 AM
[NMR paper] Interaction of the E2 and E3 components of the pyruvate dehydrogenase multienzyme com Interaction of the E2 and E3 components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus. Use of a truncated protein domain in NMR spectroscopy. Related Articles Interaction of the E2 and E3 components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus. Use of a truncated protein domain in NMR spectroscopy. FEBS J. 2005 Jan;272(1):259-68 Authors: Allen MD, Broadhurst RW, Solomon RG, Perham RN A (15)N-labelled peripheral-subunit binding domain (PSBD) of the dihydrolipoyl...	nmrlearner	Journal club	0	11-24-2010 11:14 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off