Related ArticlesConstant-time multidimensional electrophoretic NMR.
J Magn Reson. 2002 Jun;156(2):181-6
Authors: Li E, He Q
Multidimensional electrophoretic NMR (ENMR) has been introduced to determine structures of coexisting proteins and protein conformations in solution. Signals of different proteins are separated in a new dimension of electrophoretic flow according to their characteristic electrophoretic mobilities. The electrophoretic interferograms have been generated in the flow dimension in two approaches by incrementing either the amplitude or the duration of the electric field. The ENMR method of incrementing the duration of the electric field, however, introduces severe signal decays due to molecular diffusion and spin relaxation, limiting the effectiveness of the method. In this study, an improved method of constant-time multidimensional ENMR (CT-ENMR) has been proposed and successfully tested. The time delays between the magnetic field gradients and the RF pulses are kept constant in this new method so that the molecular diffusion and spin relaxation processes contribute to only a constant factor of signal amplitude. As an alternative approach of incrementing the amplitude of the electric field, this novel method significantly enhances our capability and potential in characterizing structural changes of interacting proteins during biological signaling processes. The CT-ENMR method is particularly useful in studies where the amplitude-incrementing of the electric field is not optimal. For example, the CT-ENMR method is superior when the electric field is applied in the direction not parallel to the static magnetic field B(0) to the xy-magnetization. The new method was successfully demonstrated with a sample solution containing 100 mM 4,9-dioxa-1,12-dodecanediamine and 100 mM L-aspartic acid in D(2)O.
Monitoring Mechanistic Details in the Synthesis of Pyrimidines via Real-Time, Ultrafast Multidimensional NMR Spectroscopy
Monitoring Mechanistic Details in the Synthesis of Pyrimidines via Real-Time, Ultrafast Multidimensional NMR Spectroscopy
Zulay D. Pardo, Gregory L. Olsen, Mari?a Encarnacio?n Ferna?ndez-Valle, Lucio Frydman, Roberto Marti?nez-A?lvarez and Antonio Herrera
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja210154g/aop/images/medium/ja-2011-10154g_0006.gif
Journal of the American Chemical Society
DOI: 10.1021/ja210154g
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/tEFGGh16-DU
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01-28-2012 05:27 AM
[Question from NMRWiki Q&A forum] How can I calculate a carbon-proton coupling constant for a molecule?
How can I calculate a carbon-proton coupling constant for a molecule?
I'm trying to explain a missing HMBC peak, and having a coupling constant less than 10 Hz would do that nicely. It's a formamidine derivative with a 3 bond correlation N=CHNC The C is a quaternary carbon in a benzene ring. Any help would be appreciated.
Check if somebody has answered this question on NMRWiki QA forum
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02-04-2011 07:12 PM
[NMR paper] Trp repressor-operator binding: NMR and electrophoretic mobility shift studies of the
Trp repressor-operator binding: NMR and electrophoretic mobility shift studies of the effect of DNA sequence and corepressor binding on two Trp repressor-operator complexes.
Related Articles Trp repressor-operator binding: NMR and electrophoretic mobility shift studies of the effect of DNA sequence and corepressor binding on two Trp repressor-operator complexes.
Biochemistry. 2002 Dec 17;41(50):14866-78
Authors: Jaseja M, Jeeves M, Hyde EI
In Trp repressor-DNA complexes, most interactions either occur with phosphate groups or are...
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11-24-2010 08:58 PM
[NMR paper] Convection compensated electrophoretic NMR.
Convection compensated electrophoretic NMR.
Related Articles Convection compensated electrophoretic NMR.
J Magn Reson. 2001 Jun;150(2):126-31
Authors: He Q, Wei Z
A novel method of convection compensated ENMR (CC-ENMR) has been developed to detect electrophoretic motion of ionic species in the presence of bulk solution convection. This was accomplished using a gradient moment nulling technique to remove spectral artifacts from heat-induced convection and using the polarity switch of the applied electric field to retain spin phase modulations...
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11-19-2010 08:32 PM
[NMR paper] Three-dimensional electrophoretic NMR correlation spectroscopy.
Three-dimensional electrophoretic NMR correlation spectroscopy.
Related Articles Three-dimensional electrophoretic NMR correlation spectroscopy.
J Magn Reson. 2000 Dec;147(2):361-5
Authors: He Q, Lin W, Liu Y, Li E
A novel method of three-dimensional electrophoretic NMR correlation spectroscopy (3D EP-COSY) has been proposed, developed, and implemented. It has a demonstrated potential of facilitating simultaneous structural assignments of multiple proteins in mixtures. The principle is to add a pulsed DC electric field that introduces a new...
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11-19-2010 08:29 PM
[NMR paper] Capillary array electrophoretic NMR of proteins in biological buffer solutions.
Capillary array electrophoretic NMR of proteins in biological buffer solutions.
Related Articles Capillary array electrophoretic NMR of proteins in biological buffer solutions.
J Magn Reson. 1999 Dec;141(2):355-9
Authors: He Q, Liu Y, Sun H, Li E
The capillary array electrophoretic NMR (CA-ENMR) was developed to study protein mixtures in biological buffer solutions of high ionic strength. By enhancing the strength of the effective electric field across the sample, the technique permits the detection of the electrophoretic motion of 1 mM...
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11-18-2010 08:31 PM
Improved Resolution in Dipolar NMR Spectra Using Constant Time Evolution PISEMA Exper
Improved Resolution in Dipolar NMR Spectra Using Constant Time Evolution PISEMA Experiment.
Related Articles Improved Resolution in Dipolar NMR Spectra Using Constant Time Evolution PISEMA Experiment.
Chem Phys Lett. 2010 Jul 9;494(1-3):104-110
Authors: Gopinath T, Veglia G
The atomic structure of small molecules and polypeptides can be attained from anisotropic NMR parameters such as dipolar couplings (DC) and chemical shifts (CS). Separated local field experiments resolve DC and CS correlations into two dimensions. However, crowded NMR...
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09-05-2010 05:53 AM
[NMR900 blog] Multidimensional NMR Methods for the Solution State
Multidimensional NMR Methods for the Solution State
edited by Gareth A. Morris and James W. Emsley
Hardcover: 580 pages
Publisher: Wiley; June 2010
Language: English
ISBN: 978-0470770757
http://www.amazon.com/dp/0470770759
http://www.amazon.ca/dp/0470770759