Related ArticlesConformational studies of neurohypophyseal hormones analogues with glycoconjugates by NMR spectroscopy.
J Pept Sci. 2014 Jun;20(6):406-14
Authors: Lubecka EA, Sikorska E, Marcinkowska A, Ciarkowski J
Abstract
Two glycosylated peptides have been studied using NMR spectroscopy supported by molecular modeling. Peptide I is an oxytocin (OT) analogue in which glutamine 4 was replaced by serine with attached ?-d-mannose through the oxygen ? atom, whereas peptide II is a lysine-vasopressin (LVP) analogue with lysine 8 side chain modified by the attachment of glucuronic acid through an amide bond. Both peptides exhibit very weak uterotonic effect and are less susceptible to proteolytic degradation than the mother hormones. Additionally, peptide II reveals very weak pressor and antidiuretic activities. Our results have shown that the conformational preferences of glycosylated analogues are highly similar to those of their respective mother hormones. OT glycosylated analogue (I) exhibits a 3,4 ?-turn characteristic of OT-like peptides, and vasopressin-glycosylated analogue (II) exhibits ?-turns typical of vasopressin-like peptides. Therefore, the lack of binding of the glycosylated analogues to the receptors can be attributed to a steric interference between the carbohydrate moieties and the receptors. We also consider this to be the reason of the very low activity of the analyzed glycopeptides. We expect that results from these studies will be helpful in designing new OT-like and vasopressin-like drugs.
[NMR paper] Structural studies of adipokinetic hormones in water and DPC micelle solution using NMR distance restrained molecular dynamics.
Structural studies of adipokinetic hormones in water and DPC micelle solution using NMR distance restrained molecular dynamics.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Structural studies of adipokinetic hormones in water and DPC micelle solution using NMR distance restrained molecular dynamics.
Peptides. 2014 Mar;53:270-7
Authors: Jackson GE, Gamieldien R, Mugumbate G, Gäde G
Abstract
Melme-CC (pGlu-Leu-Asn-Tyr-Ser-Pro-Asp-Trp...
nmrlearner
Journal club
0
12-19-2014 11:42 AM
Targeting of p53 Peptide Analogues to Anti-Apoptotic Bcl-2 Family Proteins as Revealed by NMR Spectroscopy
Targeting of p53 Peptide Analogues to Anti-Apoptotic Bcl-2 Family Proteins as Revealed by NMR Spectroscopy
Publication date: Available online 14 December 2013
Source:Biochemical and Biophysical Research Communications</br>
Author(s): Jae-Sun Shin , Ji-Hyang Ha , Seung-Wook Chi</br>
Inhibition of the interaction between the p53 tumor suppressor and its negative regulator MDM2 is of great importance to cancer therapy. The anti-apoptotic Bcl-2 family proteins are also attractive anti-cancer molecular targets, as they are key regulators of apoptotic cell death....
nmrlearner
Journal club
0
12-14-2013 04:48 PM
[NMR paper] Micelle-bound conformations of neurohypophyseal hormone analogues modified with a C?-disubstituted residue: NMR and molecular modelling studies.
Micelle-bound conformations of neurohypophyseal hormone analogues modified with a C?-disubstituted residue: NMR and molecular modelling studies.
Related Articles Micelle-bound conformations of neurohypophyseal hormone analogues modified with a C?-disubstituted residue: NMR and molecular modelling studies.
J Biomol Struct Dyn. 2013;31(7):748-64
Authors: Sikorska E, Kwiatkowska A
Abstract
In this study, by applying a combined approach of NMR measurements and molecular modelling, the conformations and the interactions with membrane-like...
nmrlearner
Journal club
0
11-07-2013 11:06 AM
[NMR paper] A Comparative CEST NMR Study of Slow Conformational Dynamics of Small GTPases Complexed with GTP and GTP Analogues.
A Comparative CEST NMR Study of Slow Conformational Dynamics of Small GTPases Complexed with GTP and GTP Analogues.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles A Comparative CEST NMR Study of Slow Conformational Dynamics of Small GTPases Complexed with GTP and GTP Analogues.
Angew Chem Int Ed Engl. 2013 Aug 22;
Authors: Long D, Marshall CB, Bouvignies G, Mazhab-Jafari MT, Smith MJ, Ikura M, Kay LE
Abstract
...
nmrlearner
Journal club
0
09-17-2013 11:36 PM
Dynamics and interactions of glycoconjugates probed by stable-isotope-assisted NMR spectroscopy.
Dynamics and interactions of glycoconjugates probed by stable-isotope-assisted NMR spectroscopy.
Dynamics and interactions of glycoconjugates probed by stable-isotope-assisted NMR spectroscopy.
Methods Enzymol. 2010;478:305-22
Authors: Yamaguchi Y, Kato K
Unique advantages offered by nuclear magnetic resonance (NMR) spectroscopy provide high-resolution information not only on structures but also on dynamics and interactions of glycoconjugates in solution. These benefits are further enhanced by applying stable-isotope-labeling techniques, which we...
nmrlearner
Journal club
0
12-31-2010 07:03 PM
[NMR paper] Conformational analysis of peptide analogues of silkmoth chorion protein segments usi
Conformational analysis of peptide analogues of silkmoth chorion protein segments using CD, NMR and molecular modelling.
Related Articles Conformational analysis of peptide analogues of silkmoth chorion protein segments using CD, NMR and molecular modelling.
J Pept Sci. 2004 Jun;10(6):381-92
Authors: Benaki DC, Mikros E, Hamodrakas SJ
Silkmoth proteins secreted from the follicular cells that surround the oocyte form a large extracellular assembly which is important for protecting and sustaining the structure of the oocyte and the developing...
nmrlearner
Journal club
0
11-24-2010 09:51 PM
[NMR paper] Conformational studies of microcystin-LR using NMR spectroscopy and molecular dynamic
Conformational studies of microcystin-LR using NMR spectroscopy and molecular dynamics calculations.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Conformational studies of microcystin-LR using NMR spectroscopy and molecular dynamics calculations.
Biochemistry. 1996 Mar 12;35(10):3197-205
Authors: Trogen GB, Annila A, Eriksson J, Kontteli M, Meriluoto J, Sethson I, Zdunek J, Edlund U
NMR spectroscopy in aqueous and dimethyl sulfoxide/water solutions is used to determine the...
nmrlearner
Journal club
0
08-22-2010 02:27 PM
[NMR paper] 1H-NMR studies on association of mRNA cap-analogues with tryptophan-containing peptid
1H-NMR studies on association of mRNA cap-analogues with tryptophan-containing peptides.
Related Articles 1H-NMR studies on association of mRNA cap-analogues with tryptophan-containing peptides.
Biochim Biophys Acta. 1996 Mar 7;1293(1):97-105
Authors: Stolarski R, Sitek A, StepiÅ?ski J, Jankowska M, Oksman P, Temeriusz A, Darzynkiewicz E, Lönnberg H, Shugar D
1H-NMR spectroscopy was applied to a study of the mode of interaction, in aqueous medium in the pH range 5.2-8.5 and at low and high temperatures, between several mono- and dinucleotide...
Conformational studies of neurohypophyseal hormones analogues with glycoconjugates by NMR spectroscopy.
Linear Mode
