Magic-angle spinning (MAS) is mandatory in solid-state NMR experiments to achieve resolved spectra. In rare cases, instabilities in the rotation or damage of either the rotor or the rotor cap can lead to a so called â??rotor crashâ?? involving a disintegration of the sample container and possibly the release of an aerosol or of dust. We present a modified design of a 3.2Â*mm probe with a confining chamber which in case of a rotor crash prevents the release of aerosols and possibly hazardous materials. 1D and 2D NMR experiments show that such a hazardous material-confining MAS probe (â??CONFINE-MASâ?? probe) has a similar sensitivity compared to a standard probe and performs equally well in terms of spinning stability. We illustrate the CONFINE-MAS probe properties and performance by application to a fungal amyloid.
Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale - Phys.Org
Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale - Phys.Org
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Phys.Org
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Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale
Phys.Org
"Just as we are always moving our arms and legs about, proteins are very dynamic. They do not stand still," Polenova says. "These motions are essential to their biological function, and NMR spectroscopy is the only technique...
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12-02-2017 02:54 PM
On the use of ultracentrifugal devices for routine sample preparation in biomolecular magic-angle-spinning NMR
On the use of ultracentrifugal devices for routine sample preparation in biomolecular magic-angle-spinning NMR
Abstract
A number of recent advances in the field of magic-angle-spinning (MAS) solid-state NMR have enabled its application to a range of biological systems of ever increasing complexity. To retain biological relevance, these samples are increasingly studied in a hydrated state. At the same time, experimental feasibility requires the sample preparation process to attain a high sample concentration within the final MAS rotor. We discuss these...
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02-22-2017 06:28 PM
Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale - UD Daily
Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale - UD Daily
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Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic scale
UD Daily
5:30 p.m., Nov. 24, 2015--A latticework of tiny tubes called microtubules gives your cells their shape and also acts like a railroad track that essential proteins travel on. But if there is a glitch in the connection between...
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07-18-2016 03:40 AM
Magnetic resonance imaging of DNP enhancements in a rotor spinning at the magic angle
From The DNP-NMR Blog:
Magnetic resonance imaging of DNP enhancements in a rotor spinning at the magic angle
Perras, F.A., T. Kobayashi, and M. Pruski, Magnetic resonance imaging of DNP enhancements in a rotor spinning at the magic angle. J Magn Reson, 2016. 264: p. 125-30.
http://www.ncbi.nlm.nih.gov/pubmed/26920838
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05-07-2016 03:19 AM
Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic ... - Phys.Org
Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic ... - Phys.Org
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Phys.Org
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Magic-angle-spinning NMR used to probe protein/microtubule assembly at atomic ...
Phys.Org
The research team used magic-angle-spinning nuclear magnetic resonance spectrometry (NMR) in the Department of Chemistry and Biochemistry at UD to unveil the structure of the CAP-Gly protein assembled on polymerized microtubules....
[NMR paper] Experiments Optimized for Magic Angle Spinning and Oriented Sample Solid-State NMR of Proteins.
Experiments Optimized for Magic Angle Spinning and Oriented Sample Solid-State NMR of Proteins.
Related Articles Experiments Optimized for Magic Angle Spinning and Oriented Sample Solid-State NMR of Proteins.
J Phys Chem B. 2013 Sep 17;
Authors: Das BB, Lin EC, Opella SJ
Abstract
Structure determination by solid-state NMR of proteins is rapidly advancing as result of recent developments of samples, experimental methods, and calculations. There are a number of different solid-state NMR approaches that utilize stationary, aligned samples or...
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09-21-2013 06:50 PM
Magic Angle Spinning and Oriented Sample Solid-State NMR Structural Restraints Combine for Influenza A M2 Protein Functional Insights
Magic Angle Spinning and Oriented Sample Solid-State NMR Structural Restraints Combine for Influenza A M2 Protein Functional Insights
Thach V. Can, Mukesh Sharma, Ivan Hung, Peter L. Gor’kov, William W. Brey and Timothy A. Cross
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja3004039/aop/images/medium/ja-2012-004039_0004.gif
Journal of the American Chemical Society
DOI: 10.1021/ja3004039
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/xappAmN-wb8
CONFINE-MAS: a magic-angle spinning NMR probe that confines the sample in case of a rotor explosion
Linear Mode
