[NMR paper] Comprehensive characterization of higher order structure changes in methionine oxidized monoclonal antibodies via NMR chemometric analysis and biophysical approaches
Comprehensive characterization of higher order structure changes in methionine oxidized monoclonal antibodies via NMR chemometric analysis and biophysical approaches
The higher order structure (HOS) of monoclonal antibodies (mAbs) is an important quality attribute with strong contribution to clinically relevant biological functions and drug safety. Due to the multi-faceted nature of HOS, the synergy of multiple complementary analytical approaches can substantially improve the understanding, accuracy, and resolution of HOS characterization. In this study, we applied one- and two-dimensional (1D and 2D) nuclear magnetic resonance (NMR) spectroscopy coupled...
[NMR paper] Application of NMR and Chemometric Analyses to Better Understand the Quality Attributes in pH and Thermally Degraded Monoclonal Antibodies
Application of NMR and Chemometric Analyses to Better Understand the Quality Attributes in pH and Thermally Degraded Monoclonal Antibodies
CONCLUSION: The chemometric analyses support the lack of statistical differences in the structure of pH-thermal stressed adalimumab, however, it reveals conformational changes or chemical modifications in trastuzumab after forced degradation. Application of chemometrics in comparative NMR studies enables HOS characterization and showcases the sensitivity and specificity in detecting differences in the spectra of mAbs after pH-thermal forced degradation...
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10-06-2023 10:37 PM
[NMR paper] 2D NMR Analysis as a Sensitive Tool for Evaluating the Higher-Order Structural Integrity of Monoclonal Antibody against COVID-19
2D NMR Analysis as a Sensitive Tool for Evaluating the Higher-Order Structural Integrity of Monoclonal Antibody against COVID-19
The higher-order structure (HOS) of protein therapeutics has been confirmed as a critical quality parameter. In this study, we compared 2D ¹H-^(13)C ALSOFAST-HMQC NMR spectra with immunochemical ELISA-based analysis to evaluate their sensitivity in assessing the HOS of a potent human monoclonal antibody (mAb) for the treatment of coronavirus disease 2019 (COVID-19). The study confirmed that the methyl region of the 2D ¹H-^(13)C NMR spectrum is sensitive to...
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10-28-2022 07:57 AM
[NMR paper] Assessment of the Higher-Order Structure of Formulated Monoclonal Antibody Therapeutics by 2D Methyl Correlated NMR and Principal Component Analysis.
Assessment of the Higher-Order Structure of Formulated Monoclonal Antibody Therapeutics by 2D Methyl Correlated NMR and Principal Component Analysis.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7388-69-wiley-full-text.png Assessment of the Higher-Order Structure of Formulated Monoclonal Antibody Therapeutics by 2D Methyl Correlated NMR and Principal Component Analysis.
Curr Protoc Protein Sci. 2020 Jun;100(1):e105
Authors: Arbogast LW, Delaglio F, Brinson RG, Marino JP
Abstract
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05-16-2020 02:10 AM
[NMR paper] Comparative Analysis of 1D PROFILE and 2D 1H,13C Methyl NMR Methods for Characterization of the Higher Order Structure of IgG1 Monoclonal Antibodies.
Comparative Analysis of 1D PROFILE and 2D 1H,13C Methyl NMR Methods for Characterization of the Higher Order Structure of IgG1 Monoclonal Antibodies.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Comparative Analysis of 1D PROFILE and 2D 1H,13C Methyl NMR Methods for Characterization of the Higher Order Structure of IgG1 Monoclonal Antibodies.
Anal Chem. 2020 Apr 08;:
Authors: Elliott KW, Ghasriani H, Wikstrom M, Giddens JP, Aubin Y, Delaglio F, Marino JP, Arbogast L
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04-09-2020 07:27 PM
Quality assessment of biologics: higher order structure analysis using NMR - European Pharmaceutical Review
Quality assessment of biologics: higher order structure analysis using NMR European Pharmaceutical ReviewThe webinar will describe how 2D NMR can offer protein biochemists unprecedented analytical precision. By assigning just one spectrum, users can obtain a ...
Quality assessment of biologics: higher order structure analysis using NMR - European Pharmaceutical Review
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01-07-2019 05:49 AM
[NMR paper] Effect of Polysorbate 20 and Polysorbate 80 on the Higher Order Structure of a Monoclonal Antibody and its Fab and Fc Fragments Probed Using 2D NMR.
Effect of Polysorbate 20 and Polysorbate 80 on the Higher Order Structure of a Monoclonal Antibody and its Fab and Fc Fragments Probed Using 2D NMR.
Effect of Polysorbate 20 and Polysorbate 80 on the Higher Order Structure of a Monoclonal Antibody and its Fab and Fc Fragments Probed Using 2D NMR.
J Pharm Sci. 2017 Aug 23;:
Authors: Singh SM, Bandi S, Jones DNM, Mallela KMG
Abstract
We examined how polysorbate 20 (PS20; Tween 20) and polysorbate 80 (PS80; Tween 80) affect the higher order structure of a monoclonal antibody (mAb)...
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08-28-2017 10:59 AM
[NMR paper] Simple NMR methods for evaluating higher order structures of monoclonal antibody therapeutics with quinary structure.
Simple NMR methods for evaluating higher order structures of monoclonal antibody therapeutics with quinary structure.
Related Articles Simple NMR methods for evaluating higher order structures of monoclonal antibody therapeutics with quinary structure.
J Pharm Biomed Anal. 2016 Jun 7;128:398-407
Authors: Chen K, Long DS, Lute SC, Levy MJ, Brorson KA, Keire DA
Abstract
Monoclonal antibody (mAb) drugs constitute the largest class of protein therapeutics currently on the market. Correctly folded protein higher order structure...
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06-28-2016 06:25 PM
Higher Order Amyloid Fibril Structure by MAS NMR and DNP Spectroscopy
From The DNP-NMR Blog:
Higher Order Amyloid Fibril Structure by MAS NMR and DNP Spectroscopy
Debelouchina, G.T., et al., Higher Order Amyloid Fibril Structure by MAS NMR and DNP Spectroscopy. J Am Chem Soc, 2013. 135(51): p. 19237-47.
http://www.ncbi.nlm.nih.gov/pubmed/24304221
Comprehensive characterization of higher order structure changes in methionine oxidized monoclonal antibodies via NMR chemometric analysis and biophysical approaches
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