An RNA aptamer that strongly binds to a target molecule has the potential to be a nucleic acid drug inside living human cells. To investigate and improve this potential, it is critical to elucidate the structure and interaction of RNA aptamers inside living cells. We examined an RNA aptamer for HIV-1 Tat (TA), which had been found to trap Tat and repress its function in living human cells. We first used in vitro NMR to examine the interaction between TA and a part of Tat containing the binding...
[NMR paper] Observation of nucleic acids inside living human cells by in-cell NMR spectroscopy.
Observation of nucleic acids inside living human cells by in-cell NMR spectroscopy.
Observation of nucleic acids inside living human cells by in-cell NMR spectroscopy.
Biophys Physicobiol. 2020;17:36-41
Authors: Yamaoki Y, Nagata T, Sakamoto T, Katahira M
Abstract
The intracellular environment is highly crowded with biomacromolecules such as proteins and nucleic acids. Under such conditions, the structural and biophysical features of nucleic acids have been thought to be different from those in vitro. To obtain high-resolution...
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10-29-2020 10:21 AM
[NMR paper] Recording In-Cell NMR-Spectra in Living Mammalian Cells.
Recording In-Cell NMR-Spectra in Living Mammalian Cells.
Related Articles Recording In-Cell NMR-Spectra in Living Mammalian Cells.
Methods Mol Biol. 2020;2141:857-871
Authors: Mate?ko-Burmann I, Burmann BM
Abstract
At the foundation of many cellular processes as well as a large number of diseases is the (mis)folding of important intrinsically disordered proteins (IDPs). Despite tremendous scientific efforts, the factors driving their structural changes within the cellular context remain poorly understood. In-cell NMR...
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[NMR paper] Recent progress of in-cell NMR of nucleic acids in living human cells.
Recent progress of in-cell NMR of nucleic acids in living human cells.
Related Articles Recent progress of in-cell NMR of nucleic acids in living human cells.
Biophys Rev. 2020 Mar 06;:
Authors: Yamaoki Y, Nagata T, Sakamoto T, Katahira M
Abstract
The inside of living cells is highly crowded with biological macromolecules. It has long been considered that the properties of nucleic acids and proteins, such as their structures, dynamics, interactions, and enzymatic activities, in intracellular environments are different from those...
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03-09-2020 01:12 PM
[NMR paper] The first successful observation of in-cell NMR signals of DNA and RNA in living human cells.
The first successful observation of in-cell NMR signals of DNA and RNA in living human cells.
Related Articles The first successful observation of in-cell NMR signals of DNA and RNA in living human cells.
Phys Chem Chem Phys. 2017 Oct 12;:
Authors: Yamaoki Y, Kiyoishi A, Miyake M, Kano F, Murata M, Nagata T, Katahira M
Abstract
In order to understand intracellular biological events, information on the structure, dynamics and interaction of proteins and nucleic acids in living cells is of crucial importance. In-cell NMR is a...
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10-14-2017 02:04 AM
[NMR paper] Protein dynamics in living cells studied by in-cell NMR spectroscopy.
Protein dynamics in living cells studied by in-cell NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Protein dynamics in living cells studied by in-cell NMR spectroscopy.
FEBS Lett. 2013 Jan 11;
Authors: Li C, Liu M
Abstract
Most proteins function in cells where protein concentrations can reach 400g/l. However, most quantitative studies of protein properties are performed in idealized, dilute conditions. Recently developed in-cell NMR techniques...
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02-03-2013 10:22 AM
Protein dynamics in living cells studied by in-cell NMR spectroscopy
Protein dynamics in living cells studied by in-cell NMR spectroscopy
Available online 11 January 2013
Publication year: 2013
Source:FEBS Letters</br>
</br>
Most proteins function in cells where protein concentrations can reach 400g/l. However, most quantitative studies of protein properties are performed in idealized, dilute conditions. Recently developed in-cell NMR techniques can provide protein structure and other biophysical properties inside living cells at atomic resolution. Here we review how protein dynamics, including global and internal motions have been...
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02-03-2013 10:13 AM
On-Cell MAS NMR: PhysiologicalClues from Living Cells
On-Cell MAS NMR: PhysiologicalClues from Living Cells
Giorgia Zandomeneghi, Karin Ilg, Markus Aebi and Beat H. Meier
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja307467p/aop/images/medium/ja-2012-07467p_0007.gif
Journal of the American Chemical Society
DOI: 10.1021/ja307467p
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/PBl2HVolQkY
Complex Formation of an RNA Aptamer with a Part of HIV-1 Tat through Induction of Base Triples in Living Human Cells Proven by In-Cell NMR
Linear Mode
