NMR paper Complete resolution of the solid-state NMR spectrum of a uniformly 15N-labeled membra

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[NMR paper] Complete resolution of the solid-state NMR spectrum of a uniformly 15N-labeled membra

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NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

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RCI

Interactions from chemical shifts:

HADDOCK

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RDCs:

Pseudocontact shifts:

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SAVES2 or SAVES4

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Methyl S2

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ArShift- Aromatic

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Shifty

Camcoil

Poulsen_rc_CS

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MAXOCC

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Protein solubility:

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08-22-2010, 05:08 PM

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Complete resolution of the solid-state NMR spectrum of a uniformly 15N-labeled membra

Complete resolution of the solid-state NMR spectrum of a uniformly 15N-labeled membrane protein in phospholipid bilayers.

Related Articles Complete resolution of the solid-state NMR spectrum of a uniformly 15N-labeled membrane protein in phospholipid bilayers.

Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8551-6

Authors: Marassi FM, Ramamoorthy A, Opella SJ

Complete resolution of the amide resonances in a three-dimensional solid-state NMR correlation spectrum of a uniformly 15N-labeled membrane protein in oriented phospholipid bilayers is demonstrated. The three orientationally dependent frequencies, 1H chemical shift, 1H-15N dipolar coupling, and 15N chemical shift, associated with each amide resonance are responsible for resolution among resonances and provide sufficient angular restrictions for protein structure determination. Because the protein is completely immobilized by the phospholipids on the relevant NMR time scales (10 kHz), the linewidths will not degrade in the spectra of larger proteins. Therefore, these results demonstrate that solid-state NMR experiments can overcome the correlation time problem and extend the range of proteins that can have their structures determined by NMR spectroscopy to include uniformly 15N-labeled membrane proteins in phospholipid bilayers.

PMID: 9238014 [PubMed - indexed for MEDLINE]

Source: PubMed

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