The distinctive features of the physics-based probes used in understanding the structure of matter focusing on biological sciences, but not exclusively, are described in the modern context. This is set in a wider scope of holistic biology and the scepticism about `reductionism', what is called the `molecular level', and how to respond constructively. These topics will be set alongside the principles of accuracy and precision, and their boundaries. The combination of probes and their application...
Accuracy and precision of proteinā??ligand interaction kinetics determined from chemical shift titrations
Accuracy and precision of proteinā??ligand interaction kinetics determined from chemical shift titrations
Abstract NMR-monitored chemical shift titrations for the study of weak proteinā??ligand interactions represent a rich source of information regarding thermodynamic parameters such as dissociation constants (K D ) in the micro- to millimolar range, populations for the free and ligand-bound states, and the kinetics of interconversion between states, which are typically within the fast exchange regime on the NMR timescale. We recently developed two chemical shift titration methods...
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[NMR paper] Assessing precision and accuracy of protein structures derived from NMR data.
Assessing precision and accuracy of protein structures derived from NMR data.
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Proteins. 2005 Jun 1;59(4):655-61
Authors: Snyder DA, Bhattacharya A, Huang YJ, Montelione GT
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[NMR paper] High precision NMR structure and function of the RING-H2 finger domain of EL5, a rice
High precision NMR structure and function of the RING-H2 finger domain of EL5, a rice protein whose expression is increased upon exposure to pathogen-derived oligosaccharides.
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J Biol Chem. 2003 Apr 25;278(17):15341-8
Authors: Katoh S, Hong C, Tsunoda Y, Murata K, Takai R, Minami E, Yamazaki T, Katoh E
EL5, a RING-H2 finger protein, is rapidly induced by...
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[NMR paper] Accuracy and precision of NMR relaxation experiments and MD simulations for character
Accuracy and precision of NMR relaxation experiments and MD simulations for characterizing protein dynamics.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_120x27.gif Related Articles Accuracy and precision of NMR relaxation experiments and MD simulations for characterizing protein dynamics.
Proteins. 1997 Aug;28(4):481-93
Authors: Philippopoulos M, Mandel AM, Palmer AG, Lim C
Model-free parameters obtained from nuclear magnetic resonance (NMR) relaxation...
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08-22-2010 05:08 PM
[NMR paper] An assessment of the precision and accuracy of protein structures determined by NMR.
An assessment of the precision and accuracy of protein structures determined by NMR. Dependence on distance errors.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles An assessment of the precision and accuracy of protein structures determined by NMR. Dependence on distance errors.
J Mol Biol. 1994 Jun 24;239(5):601-7
Authors: Zhao D, Jardetzky O
We tested the dependence of the accuracy and precision of calculated NMR structures on the errors of the distance constraints...
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08-22-2010 03:33 AM
[NMR paper] An assessment of the precision and accuracy of protein structures determined by NMR.
An assessment of the precision and accuracy of protein structures determined by NMR. Dependence on distance errors.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles An assessment of the precision and accuracy of protein structures determined by NMR. Dependence on distance errors.
J Mol Biol. 1994 Jun 24;239(5):601-7
Authors: Zhao D, Jardetzky O
We tested the dependence of the accuracy and precision of calculated NMR structures on the errors of the distance constraints...
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08-22-2010 03:33 AM
Precision and Accuracy of NMR Structures - PSI
Precision and Accuracy of NMR Structures - a presentation by the Protein Structure Initiative - is available to read from here:
http://journals.iucr.org/services/nmr/precision.pdf
More information about the PSI can be found here:
http://www.structuralgenomics.org/
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09-10-2008 05:32 PM
Assessing precision and accuracy of protein struct
Editor's Corner
Assessing precision and accuracy of protein structures derived from NMR data
David A. Snyder 1 2, Aneerban Bhattacharya 1 2, Yuanpeng J. Huang 1 2, Gaetano T. Montelione 1 2 *
1Center for Advanced Biotechnology and Medicine, Department of Molecular Biology and Biochemistry, and Northeast Structural Genomics Consortium, Rutgers University, Piscataway, NJ 08854
2Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Piscataway, NJ 08854
*Correspondence to Gaetano T. Montelione, CABM-Rutgers University, 679 Hoes Lane, Piscataway, NJ...
Combining X-rays, neutrons and electrons, and NMR, for precision and accuracy in structure-function studies
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