[NMR paper] Combining Solid-State NMR with Structural and Biophysical Techniques to Design Challenging Protein-Drug Conjugates
Combining Solid-State NMR with Structural and Biophysical Techniques to Design Challenging Protein-Drug Conjugates
Several protein-drug conjugates are currently being used in cancer therapy. These conjugates rely on cytotoxic organic compounds that are covalently attached to the carrier proteins or that interact with them via non-covalent interactions. Human transthyretin (TTR), a physiological protein, has already been identified as a possible carrier protein for the delivery of cytotoxic drugs. Here we show the structure-guided development of a new stable cytotoxic molecule based on a...
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06-06-2023 09:06 PM
[NMR paper] Structure Determination of Challenging Protein-Peptide Complexes Combining NMR Chemical Shift Data and Molecular Dynamics Simulations
Structure Determination of Challenging Protein-Peptide Complexes Combining NMR Chemical Shift Data and Molecular Dynamics Simulations
Intrinsically disordered regions of proteins often mediate important protein-protein interactions. However, the folding-upon-binding nature of many polypeptide-protein interactions limits the ability of modeling tools to predict the three-dimensional structures of such complexes. To address this problem, we have taken a tandem approach combining NMR chemical shift data and molecular simulations to determine the structures of peptide-protein complexes. Here,...
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03-31-2023 09:21 AM
Structure of A2aAR protein holds clues for better drug design - Drug Target Review
Structure of A2aAR protein holds clues for better drug design - Drug Target Review
Structure of A2aAR protein holds clues for better drug design Drug Target ReviewScientists have investigated a key protein used in drug design and discovered dynamic structural features that may lead to new ways to target diseases…
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01-07-2019 05:49 AM
New Structure of Key Protein Holds Clues for Better Drug Design - Drug Discovery & Development
New Structure of Key Protein Holds Clues for Better Drug Design - Drug Discovery & Development
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Drug Discovery & Development
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New Structure of Key Protein Holds Clues for Better Drug Design
Drug Discovery & Development
To solve this problem, the researchers used a technique called nuclear magnetic resonance (NMR) spectroscopy, which creates strong magnetic fields to locate the positions of probes in a sample. Wüthrich is a...
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01-29-2018 01:57 PM
Protein's Dynamic Structural Features Could Help Improve Drug Design - Technology Networks
Protein's Dynamic Structural Features Could Help Improve Drug Design - Technology Networks
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Technology Networks
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Protein's Dynamic Structural Features Could Help Improve Drug Design
Technology Networks
To solve this problem, the researchers used a technique called nuclear magnetic resonance (NMR) spectroscopy, which creates strong magnetic fields to locate the positions of probes in a sample. Wüthrich is a world-renowned...
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01-07-2018 05:03 PM
New Protein Evolution Insight Could Improve Drug Design - Drug Discovery & Development
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New Protein Evolution Insight Could Improve Drug Design
Drug Discovery & Development
The team used a variety of techniques to characterize the two versions of the enzyme, including X-ray crystallography and nuclear magnetic resonance, analyses of DHFR amino-acid sequences and evaluations of the enzyme's functionality in cells and in ...
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New Protein Evolution Insight Could Improve Drug Design - Drug Discovery & Development
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10-01-2013 09:43 AM
[NMR paper] Characterization of covalent protein conjugates using solid-state 13C NMR spectroscop
Characterization of covalent protein conjugates using solid-state 13C NMR spectroscopy.
Related Articles Characterization of covalent protein conjugates using solid-state 13C NMR spectroscopy.
Biochemistry. 1991 Jul 23;30(29):7057-62
Authors: Garbow JR, Fujiwara H, Sharp CR, Logusch EW
Cross-polarization magic-angle spinning (CPMAS) 13C NMR spectroscopy has been used to characterize covalent conjugates of alachlor, an alpha-chloroacetamide hapten, with glutathione (GSH) and bovine serum albumin (BSA). The solid-state NMR method demonstrates...
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08-21-2010 11:12 PM
[NMR paper] Characterization of covalent protein conjugates using solid-state 13C NMR spectroscop
Characterization of covalent protein conjugates using solid-state 13C NMR spectroscopy.
Related Articles Characterization of covalent protein conjugates using solid-state 13C NMR spectroscopy.
Biochemistry. 1991 Jul 23;30(29):7057-62
Authors: Garbow JR, Fujiwara H, Sharp CR, Logusch EW
Cross-polarization magic-angle spinning (CPMAS) 13C NMR spectroscopy has been used to characterize covalent conjugates of alachlor, an alpha-chloroacetamide hapten, with glutathione (GSH) and bovine serum albumin (BSA). The solid-state NMR method demonstrates...