Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register #DNPNMR
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Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register #DNPNMR
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Frederick, K.K., et al., Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register. Proc. Nat. Aca. Sci. USA, 2017. 114(14): p. 3642-3647.
http://www.pnas.org/content/114/14/3642.abstract
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04-24-2017 02:56 PM
Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register [Biophysics and Computational Biology]
Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register
Kendra K. Frederick, Vladimir K. Michaelis, Marc A. Caporini, Loren B. Andreas, Galia T. Debelouchina, Robert G. Griffin, Susan Lindquist...
Date: 2017-04-04
The yeast prion protein Sup35NM is a self-propagating amyloid. Despite intense study, there is no consensus on the organization of monomers within Sup35NM fibrils. Some studies point to a ?-helical arrangement, whereas others suggest a parallel in-register organization. Intermolecular contacts are often...
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04-04-2017 10:41 PM
[NMR paper] Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register.
Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register.
Related Articles Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register.
Proc Natl Acad Sci U S A. 2017 Mar 22;:
Authors: Frederick KK, Michaelis VK, Caporini MA, Andreas LB, Debelouchina GT, Griffin RG, Lindquist S
Abstract
The yeast prion protein Sup35NM is a self-propagating amyloid. Despite intense study, there is no consensus on...
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03-24-2017 10:14 PM
Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing
Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing
Abstract Segmental isotopic labeling is a powerful labeling tool to facilitate NMR studies of larger proteins by not only alleviating the signal overlap problem but also retaining features of uniform isotopic labeling. Although two approaches, expressed protein ligation (EPL) and protein trans-splicing (PTS), have been mainly used for segmental isotopic labeling, there has been no single example in which both approaches have been...
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07-02-2012 06:18 AM
Segmental isotope labeling of proteins for NMR structural study using a protein S tag for higher expression and solubility
Segmental isotope labeling of proteins for NMR structural study using a protein S tag for higher expression and solubility
Abstract A common obstacle to NMR studies of proteins is sample preparation. In many cases, proteins targeted for NMR studies are poorly expressed and/or expressed in insoluble forms. Here, we describe a novel approach to overcome these problems. In the protein S tag-intein (PSTI) technology, two tandem 92-residue N-terminal domains of protein S (PrS2) from Myxococcus xanthus is fused at the N-terminal end of a protein to enhance its expression and solubility. Using...
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03-08-2012 08:46 AM
[NMR paper] Combining inducible protein overexpression with NMR-grade triple isotope labeling in the cyanobacterium Anabaena sp. PCC 7120.
Combining inducible protein overexpression with NMR-grade triple isotope labeling in the cyanobacterium Anabaena sp. PCC 7120.
Related Articles Combining inducible protein overexpression with NMR-grade triple isotope labeling in the cyanobacterium Anabaena sp. PCC 7120.
Biotechniques. 2005 Sep;39(3):405-11
Authors: Desplancq D, Bernard C, Sibler AP, Kieffer B, Miguet L, Potier N, Van Dorsselaer A, Weiss E
The difficulty and expense of preparing protein samples highly enriched in stable isotopes is a bottleneck for structural studies by nuclear...
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12-01-2010 06:56 PM
[NMR paper] Segmental isotopic labeling for structural biological applications of NMR.
Segmental isotopic labeling for structural biological applications of NMR.
Related Articles Segmental isotopic labeling for structural biological applications of NMR.
Methods Mol Biol. 2004;278:47-56
Authors: Cowburn D, Shekhtman A, Xu R, Ottesen JJ, Muir TW
This chapter describes the preparation of precursor domains for the formation of multidomain segmentally labeled proteins by protein ligation.
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11-24-2010 09:25 PM
[NMR paper] Labeling of recombinant protein for NMR spectroscopy: global and specific labeling of
Labeling of recombinant protein for NMR spectroscopy: global and specific labeling of the rat liver fructose 2,6-bisphosphatase domain.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Labeling of recombinant protein for NMR spectroscopy: global and specific labeling of the rat liver fructose 2,6-bisphosphatase domain.
Protein Expr Purif. 1997 Oct;11(1):79-85
Authors: Okar DA, Felicia ND, Gui L, Lange AJ
Methods for the efficient use of the 13C-labeled nutrients,...
Combining DNP NMR with Segmental and Specific Labeling to Study the Quaternary Structures of Yeast Prion Protein Strains
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