[NMR paper] Combined H-N Cross-Polarization and Carbonyl Detection NMR Spectroscopy Allow to Record High-Resolution, High-Sensitivity Spectra of Alpha-Synuclein in Bacterial Cells
Combined H-N Cross-Polarization and Carbonyl Detection NMR Spectroscopy Allow to Record High-Resolution, High-Sensitivity Spectra of Alpha-Synuclein in Bacterial Cells
Studies of intrinsically disordered proteins (IDPs) under physiological conditions by conventional NMR methods based on proton detection are severely limited by fast proton amide solvent exchange. Carbon detection has been proposed as a solution to the exchange problem but is hampered by low sensitivity. Here, we present a protocol combining proton-nitrogen cross-polarization and carbonyl detection to record high-resolution and high-sensitivity NMR spectra of IDPs under physiological conditions....
[NMR paper] Erratum to: Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR.
Erratum to: Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR.
Related Articles Erratum to: Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR.
J Biomol NMR. 2017 Dec 11;:
Authors: Takeuchi K, Arthanari H, Shimada I, Wagner G
Abstract
The authors regret a mistake appeared in the supplement of this paper.
High sensitivity high-resolution full range relaxometry using a fast mechanical sample shuttling device and a cryo-probe
High sensitivity high-resolution full range relaxometry using a fast mechanical sample shuttling device and a cryo-probe
Abstract
Field-dependent NMR studies of bio-molecular systems using a sample shuttling hardware operating on a high-field NMR apparatus have provided valuable structural and dynamic information. We have recently published a design of a compact sample transportation device, called â??field-cyclerâ??, which was installed in a commercial spectrometer and which provided highly precise positioning and stability during high speed...
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[NMR paper] Studying Intrinsically Disordered Proteins under True In Vivo Conditions by Combined Cross-Polarization and Carbonyl-Detection NMR Spectroscopy.
Studying Intrinsically Disordered Proteins under True In Vivo Conditions by Combined Cross-Polarization and Carbonyl-Detection NMR Spectroscopy.
Related Articles Studying Intrinsically Disordered Proteins under True In Vivo Conditions by Combined Cross-Polarization and Carbonyl-Detection NMR Spectroscopy.
Angew Chem Int Ed Engl. 2016 May 9;
Authors: Lopez J, Schneider R, Cantrelle FX, Huvent I, Lippens G
Abstract
Under physiological conditions, studies of intrinsically disordered proteins (IDPs) by conventional NMR methods based...
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05-10-2016 04:13 PM
[NMR paper] Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR.
Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR.
Related Articles Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR.
J Biomol NMR. 2015 Oct 23;
Authors: Takeuchi K, Arthanari H, Shimada I, Wagner G
Abstract
Detection of (15)N in multidimensional NMR experiments of proteins has sparsely been utilized because of the low gyromagnetic ratio (?) of nitrogen and the presumed low sensitivity of such experiments. Here we show that selecting the TROSY...
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10-27-2015 12:33 PM
Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR
Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMR
Abstract
Detection of 15N in multidimensional NMR experiments of proteins has sparsely been utilized because of the low gyromagnetic ratio (γ) of nitrogen and the presumed low sensitivity of such experiments. Here we show that selecting the TROSY components of proton-attached 15N nuclei (TROSY 15NH) yields high quality spectra in high field magnets (>600Â*MHz) by taking advantage of the slow 15N transverse relaxation and compensating for the inherently low...
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10-24-2015 05:49 AM
Sparsely-sampled, high-resolution 4-D omit spectra for detection and assignment of intermolecular NOEs of protein complexes
Sparsely-sampled, high-resolution 4-D omit spectra for detection and assignment of intermolecular NOEs of protein complexes
Abstract
Unambiguous detection and assignment of intermolecular NOEs are essential for structure determination of protein complexes by NMR. Such information has traditionally been obtained with 3-D half-filtered experiments, where scalar coupling-based purging of intramolecular signals allows for selective detection of intermolecular NOEs. However, due to the large variation of 1JHC scalar couplings and limited chemical shift...
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06-19-2014 10:21 PM
TSAR: a program for automatic resonance assignment using 2D cross-sections of high dimensionality, high-resolution spectra
TSAR: a program for automatic resonance assignment using 2D cross-sections of high dimensionality, high-resolution spectra
Abstract While NMR studies of proteins typically aim at structure, dynamics or interactions, resonance assignments represent in almost all cases the initial step of the analysis. With increasing complexity of the NMR spectra, for example due to decreasing extent of ordered structure, this task often becomes both difficult and time-consuming, and the recording of high-dimensional data with high-resolution may be essential. Random sampling of the evolution time space,...
Combined H-N Cross-Polarization and Carbonyl Detection NMR Spectroscopy Allow to Record High-Resolution, High-Sensitivity Spectra of Alpha-Synuclein in Bacterial Cells
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